Українська Русский English

ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2015; 61(3): 75-80


N.M. Shpakova, N.V. Orlova, E.E. Nipot, D.I. Aleksandrova

    Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov


Sensitivity of human and animal (bovine, rat, rabbit, equine) erythrocytes to the effect of mechanical stress has been studied. Mechanical stress effect was demonstrated to result in a time-dependent (5-60 min) release of potassium cations out of mammalian erythrocytes and a partial hemolytic cell damage. Herewith the release levels of potassium ions and hemolysis did not coincide for erythrocytes of all the mammals except rabbit ones. The most sensitive to mechanical stress (60 min) by the parameters of hemolytic damage and potassium ion release were rat (32%) and bovine (66%) erythrocytes respectively, the lowest sensitive by both parameters were rabbit ones (about 20%). Implemented correlation analysis has demonstrated a statistically significant negative relation between the values of mechanical hemolysis of mammalian erythrocytes and surface-volumetric ratio of cells (rs = –0.900, P = 0.037). A feasible relationship between the content of phosphatidylethanolamine in mammalian erythrocyte membranes and the level of potassium cation loss under mechanical stress effect is under discussion.

Keywords: mammalian erythrocytes; mechanical stress; potassium cations; hemolytic damage


  1. Plasenzotti R, Stoiber B, Posch M, Windberger U. Red blood cell deformability and aggregation bihaviour in different animal species. Clin Hematol Microcirc. 2004; 31(2):105-11.
  3. Muravyov AV, Kislov NB, Tikhomirova IA, Mikhailov PB, Muravyov AA. Influence of plasma viscosity and hematocrit on deformation of erythrocytes. Russ J Biomech. 2013; 17(2):75-83 [Russian].
  5. Baskurt OK, Meiselman HJ. Red blood cell mechanical stability test. Clin Hemorheol Microcirc. 2013; 55(1):55- 62.
  7. Kameneva MV, Undar A, Aniaki JM, Watach MJ, Calhood JH, Borovetz HS. Decrease in red blood cell deformability caused by hypothermia, hemodilution, and mechanical stress: factors related to cardio pulmonary bypass. ASAIO J. 1999 Jul-Aug; 45(4):307-10.
  9. Pantaler ER, Degtyarev AV, Zhutchenko IA. Dynamic deformation of single red blood cells in hypertonic media. Theoretical and practical aspects of modern cryobiology: Collection of scientific papers. Kiev: Naukova Dumka; 1989:6-9 [Russian].
  11. Zavodnik IB, Piletskaya TP, Stepuro II. Mechanical lysis of human erythrocytes. Membranes stabilizing by plasma proteins. Ukr Biochem J. 1991;63(6):72-8 [Russian].
  12.   Fedosova SN, Kudokotseva EV. Modifying action of curantyl on the osmotic susceptibility of red blood cells. Problems of Cryobiology. 1995; (1):27-31 [Russian]  
  13. Baskurt OK, Uyuklu M, Meiselman HJ. Protection of erythrocytes from sub-hemolytic mechanical damage by nitric oxide mediated inhibition of potassium leakage. Biorheology. 2004; 41(2):79-89.
  14.   Shpakova NM, Orlova NV, Alexandrova DІ, inventors; Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, assignee. Method of erythrocyte destruction. Ukraine patent 52701, IPC8 G 01 N 33/48. 2010 00983. 2010 Sept 10. [Ukraine]  
  15. Jikuya T, Tsutsui T, Shigeta O, Sankai Y, Mitsui T. Species differences in erythrocyte mechanical fragility: comparison of human, bovine, and ovine cells. ASAIO J. 1998 Sep-Oct; 44(5):M452-5.
  17. Pohl M, Wendt MO, Pelzer S. Characterization of mechanical in vitro hemolysis and sub-hemolysis. 2: Variables of state and dimensionless characteristic values of hemolysis. Biomed Tech. (Berl.). 2000 Mar;45(3):51-6. German.
  19. Yokoyama N, Sakota D, Nagaoka E, Takatani S. Altera tions in red blood cell volume and hemoglobin concentration, viscoelastic properties, and mechanical fragility caused by continuous flow pumping in calves. Artif Organs. 2011 Aug; 35(8):791-9.
  21. Benga G. Comparative studies of water permeability of red blood cells from humans and over 30 animal species: an overview of 20 years of collaboration with Philip Kuchel. Eur Biophys J. 2013;42(1):33-46. CrossRef PubMed
  23. Bogner P, Sipos K, Ludány A, Somogyi B, Miseta A. Steady-state volumes and metabolism-independent osmotic adaptation in mammalian erythrocytes. Eur Biophys J. 2002; 31(2):145-52. CrossRef PubMed
  25. Liu L, Lei T, Bankir L, Zhao D, Gai X, Zhao X, et al. Erythrocyte permeability to urea and water: comparative study in rodents, ruminants, carnivores, humans, and birds. J Comp Physiol B. 2011 Jan;181(1):65-72.
  27. Matei H, Frentescu L, Benga G. Comparative studies of the protein composition of red blood cell membranes from eight mammalian species. J Cell Mol Med. 2000 Oct-Dec;4(4):270-6.
  29. Wessels JMC, Veerkamp JH. Some aspects of the osmotic lysis of erythrocytes III. Comparison of glycerol permeability and lipid composition of red blood cell membranes from eight mammalian species. Biochim Biophys Acta. 1973 Jan 2; 291(1):190-6.
  31. Methods of veterinary clinical laboratory diagnostics: a handbook. Kondrahina SP, editor. Moscow: ColosS; 2004. 520 p. [Russian].
  33. Betticher DC, Geiser J. Resistance of mammalian red blood cells of different size to hypertonic milieu. Comp Biochem Physiol A. 1989;93(2):429-32. CrossRef  
  34. Jones DA The importance of surface area/volume ratio to the rate of oxygen uptake by red cells. J Gen Physiol. 1979 Nov;74(5):643-6.
  36. Kowluru R, Bitensky MW, Kowluru A, Dembo M, Keaton PA, Buican T. Reversible sodium pump defect and swelling in the diabetic rat erythrocyte: Effects on filterability and implications for microangiopathy. Proc Natl Acad Sci USA. 1989 May; 86(9):3327-31.
  38. Shpakova NM. Temperature and osmotic resistance of erythrocytes of different mammalian species [dissertation]. Kharkov: Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine; 2014 [Russian].
  40. Gordienko EA, Gordienko YuE, Gordienko OI. The physico-mathematical theory of human erythrocyte hypotonic hemolysis phenomenon. Cryo Letters. 2003 Jul-Aug; 24(4):229-44.
  42. Ivkov VG, Berestovsky GN. Dynamic structure of lipid bilayer. Moscow: Nauka; 1981. 293 p. [Russian].

© National Academy of Sciences of Ukraine, Bogomoletz Institute of Physiology, 2014-2019.