COMPARATIVE STUDY OF MECHANICAL STRESS EFFECT ON HUMAN AND ANIMAL ERYTHROCYTES
N.M. Shpakova, N.V. Orlova, E.E. Nipot, D.I. Aleksandrova
Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
Sensitivity of human and animal (bovine, rat, rabbit, equine)
erythrocytes to the effect of mechanical stress has been studied.
Mechanical stress effect was demonstrated to result in a
time-dependent (5-60 min) release of potassium cations out of
mammalian erythrocytes and a partial hemolytic cell damage.
Herewith the release levels of potassium ions and hemolysis
did not coincide for erythrocytes of all the mammals except
rabbit ones. The most sensitive to mechanical stress (60
min) by the parameters of hemolytic damage and potassium
ion release were rat (32%) and bovine (66%) erythrocytes
respectively, the lowest sensitive by both parameters were
rabbit ones (about 20%). Implemented correlation analysis
has demonstrated a statistically significant negative relation
between the values of mechanical hemolysis of mammalian
erythrocytes and surface-volumetric ratio of cells (rs
P = 0.037). A feasible relationship between the content of phosphatidylethanolamine
in mammalian erythrocyte membranes
and the level of potassium cation loss under mechanical stress
effect is under discussion.
mammalian erythrocytes; mechanical stress; potassium cations; hemolytic damage
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