ROLE OF THROMBOXANE AND LEUKOTRIENES IN MECHANISMS OF CONTRACTILE REACTIONS OF PORTAL VEIN, INDUCED BY ACETYLCHLINE AND PHENYLEPHRINE
O.O. Vinogradova, P.I. Yanchuk, O.M. Pasichnichenko
NSC "Institute of Biology" Shevchenko Kyiv National University
Effects of picotamide and zileuton on tonic contractile activity
of the rat portal vein preparations, induced by acetylcholine
mol/l) and phenylephrine (5∙10-7
mol/ l) were investigated.
Conversion of arachidonic acid products (prostaglandins,
leukotrienes) synthesized by endothelial cells, plays an
important role in the local regulation of vascular tone. The
compounds formed in a cascade of enzymatic transformations
can modulate the effect of other vasoactive factors. Picotamide
mol/ l) - thromboxane receptor and thromboxane
-synthase blocker - depress acetylcholine-induction tonic
contraction of isolated segments of portal vein with intact endothelium
by 29% and norepinephrine- induction reduction of
45% relative to the control values. The obtained results indicate
a participation of thromboxane and/or endoperoxide H2 in this
reaction. Partial inhibition of the contractions by 5-lipoxygenase
blocker zileuton (4,2·10-5
mol/ l) at 23% relative to control
values suggests, that products of lipoxigenase pathways of
arachidonic acid conversion are involved in mechanisms of
specified reactions. These data indicate complex mechanisms
of regulation of vascular tone of the portal vein, which play an
important role eicosanoids. Further study of these mechanisms
is necessary for the formation of basic knowledge, as well as
to elucidate the mechanisms of occurrence and development
of pathological conditions of vessels and the development of
methods of their correction.
acetylcholine; phenylephrine; portal vein; thromboxane; leukotrienes.
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