Українська English

ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2015; 61(3): 44-50

THE INFLUENCE OF NANODISPERSE CERIUM DIOXIDE ON ONTOGENETIC CHANGES OF ANTIOXIDANT SYSTEM IN THE MUCOSA OF THE STOMACH AND COLON IN RATS

O.Yu Iefimenko 1, I.O. Savchenko 1, T.M. Falalyeyeva 1, T.V. Beregova1, N.M. Zholobak2, O.B. Shcherbakov 2, Yu.V. Malyukin 3, M. Ya Spivak 2.

  1. Taras Shevchenko National University of Kyiv;
  2. Danylo Zaboloty Institute of Microbiology and Virology National Academy of Science of Ukraine, Kyiv;
  3. Insitute for Scintillation Materials National Academy of Science of Ukraine, Kharkiv.
DOI: https://doi.org/10.15407/fz61.03.044


Abstract

It was established that with age the content of lipid peroxidation products increased in the mucosa of the stomach: Diene conjugates by 30%, products which react to thiobarbituric acid by 285% and Schif bases by 181%. Nanodisperse cerium dioxide (NCD) reduced the content of lipid peroxidation in the gastric mucosa in old rats: Diene conjugates by 43 %, products which react to thiobarbituric acid by 51% and Schif bases by 44% relative to the control group of rats given age. Similarly, it was established that the content of Diene conjugates increased by 40%, products which react to thiobarbituric acid by 114% and Schif bases by 132% in the mucosa of the colon of old rats. NDC significant reduced the content of products which react to thiobarbituric acid by 69% and Schyf basics by 132%. In the stomach superoxide dismutase (by 43%) and catalase activity (by 24%) decreases with age, while in the colon superoxide dismutase activity increases (by 43%). In the colon NCD significant decreased superoxide dismutase (by 34%) and catalase activity (by 21%) relative to controls. Thus, the NDC restores lipid peroxidation in the gastric mucosa and colon, in which develops oxidative stress with age.

Keywords: nanodisperse cerium dioxid;, lipid peroxidation; mucose of the stomach and colon.

Full text: (PDF, in Ukrainian)

References

  1. Salmon AB, Richardson A, Pérez VI. Update on the oxidative stress theory of aging: Does oxidative stress play a role in aging or healthy aging? Free Radic Biol Med. 2010; 48 (5): 642–76. CrossRef PubMed PubMedCentral
    2.  
  2. Nusbaum NJ The aging/cancer connection. Am. J. Med. Sci. 1998; 315(1): 40–9.
    3.  
  3. Celardo I, Traversa E, Ghibelli LJ. Cerium oxide nanoparticles: a promise for applications in therapy. Exp Ther Oncol. 2011; 9(1):47–51.
    4.  
  4. Wong LL, McGinnis JF. Nanoceria as bona fide catalytic antioxidants in medicine: what we know and what we want to know. Adv Exp Med Biol. 2014; 801 (1): 821–28.
    5.  
  5. Heckman KL, De Coteau W, Estevez A, Reed KJ, Costanzo W. Custom cerium oxide nanoparticles protect against a free radical mediated autoimmune degenerative disease in the brain. ACS Nano. 2013; 7(12): 10582–96. CrossRef PubMed
    6.  
  6. Lee SS, Song W, Cho M, Puppala HL, Nguyen P. Antioxidant properties of cerium oxide nanocrystals as a function of nanocrystal diameter and surface coating. ACS Nano. 2013; 7(11): 9693–703. CrossRef PubMed
    7.  
  7. Chen S, HouY, Cheng G, Zhang C, Wang S. Cerium oxide nanoparticles protect endothelial cells from apoptosis induced by oxidative stress. Biol Trace Elem Res. 2013; 154(1): 156–66. CrossRef PubMed
    8.  
  8. Jianli N, Azfer A, Rogers L, Wang X. Cardio protective effects of cerium dioxide nanoparticle in a transgenic murine model of cardiomyopathy. Cardiovasc Res. 2007; 73(3): 549–59. CrossRef PubMed PubMedCentral
    9.  
  9. Colon J, Hsieh N, Ferguson A, Kupelian P, Seal S, Jenkins DW. Cerium oxide nanoparticles protect gastrointestinal epithelium from radiation–induced damage by reduction of reactive oxygen species and upregulation of superoxide dismutase 2. Nanomedicine. 2010; 6(5): 698–705. CrossRef PubMed
    10.  
  10. Li C, Zhao W, Liu B, Xu G, Liu L. Cytotoxicity of ultrafine monodispersed nanoceria on human gastric cancer cells. J Biomed Nanotechnol. 2014; Jul;10(7):1231–41.
    11.  
  11. Ivanova OS, Shekynova TO, Ivanov VK, Scherbakov aB, Popov aL. One–step synthesis of colloidal solutions of cerium dioxide for biomedical applications. Dokl. akadem. nayk. 2011; 437 (5):638−41 [Russian].
    12.  
  12. Gavrylov VB, Gavrylova aR., Shmara NF. Measurement of diene conjugates in plasma by UV absorbance heptane and isopropanol extracts. Laboratornoe delo. 1988; 2: 60–3 [Russian].
    13.  
  13. Kolesova OE, Markin aa, Fedorova TN. Lipid peroxidation and methods for determining lipid peroxidation products in biological samples. Labor. delo. 1984; 9: 540–46 [Russian].
    14.  
  14. Stalnaya ID, Garyushvili TG. The modern methods of biocemistry. – M.: Medicina. 1977: 66–8 [Russian].
    15.  
  15. Chevari S, Chaba I, Sekey I. The role of superoxide dismutase in cell oxidative processes and method of its determination in biological material. Labor. delo. 1985; 11: 678–81.
    16.  
  16. Tyurin YuN. Distribution associated with normal. M.: Infra*M. 1998 [Russian].
    17.  
  17. Prasad RGSV, Davan R, Johti S. Cerium oxide nanoparticles protect gastrointestinal mucosa from ethanol induced gastric ulcer in in–vivo animal model. Nano Biomed Eng. 2013; 5(1):46–9.
    18.  
  18. Le Couteur DG, Blyth FM, Creasey HM. et al. The association of alanine transaminase with aging, frailty, and mortality. J Gerontol A Biol Sci Med Sci. 2010; 65(7): 712–17. CrossRef PubMed PubMedCentral
    19.  
  19. Obychova LK. Contribution of Academician NM Emanuel in the development of domestic Gerontology: free radical mechanisms in the aging process. Usp. Gerontoli.1999;3:27–31 [Russian].
    20.  
  20. Drake IM, Mapstone NP, Schorah CJ, et al. Reactive oxygen species activity and lipid peroxidation in Helicobacter pyloriassociated gastritis: relation to gastric mucosal ascorbic acid concentrations and effect of H pylori eradication. Gut. 1998; 42(6):768–71. CrossRef PubMed PubMedCentral
    21.  
  21. Pirmohamed T, Dowding JM, Singh S, Wasserman B, Heckert E. Nanoceria exhibit redox state–dependent catalase mimetic activity. Chem Commun (Camb). 2010; 46 (16): 2736–38. CrossRef PubMed PubMedCentral
    22.  
  22. Xia T, Kovochich M, Liong M, Mädler L. Comparison of the mechanism of toxicity of zinc oxide and cerium oxide nanoparticles based on dissolution and oxidative stress properties. ACS Nano. 2008;2(10): 2121–34. CrossRef PubMed PubMedCentral
    23.  

© National Academy of Sciences of Ukraine, Bogomoletz Institute of Physiology, 2014-2024.