Українська Русский English

ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2015; 61(3): 19-27


USING THE SERIAL RAMP RECORDINGS FOR RAPID TESTING OF THE GENERATING ABILITY OF IMPULSE ACTIVITY OF ISOLATED HIPPOCAMPAL NEURONS

V.A. Yavorsky, E.A. Lukyanetz

    O.O.Bogomoletz Institute of Physiology, Kyiv, National Academy of Sciences of Ukraine
DOI: https://doi.org/10.15407/fz61.03.019

Abstract

In this study we investigated changes of impulse activity of hippocampal neurons of the hippocampus by using ramp recordings. We have described the usage of serial ramp recordings of neuronal electrical activity for rapid testing of the generating ability of isolated hippocampal neurons. An analysis of the data has shown that the proposed protocol of serial ramp recordings allows to define additional characteristics of the neuronal impulse activity: (i) the thresholds for initiation of generation and suppression of the generation, (ii) the shape and amplitude of relationship between the interpulse intervals and neuronal depolarizations. The suggested stimulation protocols and related analysis are tools that can be effectively used to justify influence of chemicals or other experimental factors on the impulse activity of neurons.

References

  1. Lunko OO, Isaev DS, Maximyuk OP, Krishtal OA, Isaeva EV. The effect of enzymatic treatment using proteases on properties of persistent sodium current in CA1 piramidal neurons of rat hippocampus. Fiziol Zh. 2014; 60 (3): 75-9. [Ukrainian].
  2.  
  3. Estacion M, Waxman SG. The response of Na(V)1.3 sodium channels to ramp stimuli: multiple components and mechanisms. J Neurophysiol. 2013; 109 (2): 306-14. CrossRef PubMed
  4.  
  5. Kononenko NI, Shao LR, Dudek FE. Riluzole-sensitive slowly inactivating sodium current in rat suprachiasmatic nucleus neurons. J Neurophysiol. 2004; 91 (2): 710-8. CrossRef PubMed
  6.  
  7. Power KE, Carlin KP, Fedirchuk B. Modulation of voltage-gated sodium channels hyperpolarizes the voltage threshold for activation in spinal motoneurones. Exp Brain Res. 2012; 217 (2): 311-22. CrossRef PubMed
  8.  
  9. Wu SN, Lo YC, Shen AY, Chen BS. Contribution of noninactivating Na+ current induced by oxidizing agents to the firing behavior of neuronal action potentials: experimental and theoretical studies from NG108-15 neuronal cells. Chin J Physiol. 2011; 54 (1): 19-29. CrossRef  
  10. Cheng X, Dib-Hajj SD, Tyrrell L, Te Morsche RH, Drenth JP, Waxman SG. Deletion mutation of sodium channel Na(V)1.7 in inherited erythromelalgia: enhanced slow inactivation modulates dorsal root ganglion neuron hyperexcitability. Brain. 2011; 134 (Pt 7): 1972-86.
  11.  
  12. Estacion M, Waxman SG, Dib-Hajj SD. Effects of ranolazine on wild-type and mutant hNav1.7 channels and on DRG neuron excitability. Mol Pain. 2010; 6: 35. CrossRef PubMed PubMedCentral
  13.  
  14. Estacion M, Gasser A, Dib-Hajj SD, Waxman SG. A sodium channel mutation linked to epilepsy increases ramp and persistent current of Nav1.3 and induces hyperexcitability in hippocampal neurons. Exp Neurol. 2010; 224 (2): 362-8. CrossRef PubMed
  15.  
  16. Yan J, Melemedjian OK, Price TJ, Dussor G. Sensitization of dural afferents underlies migraine-related behavior following meningeal application of interleukin-6 (IL-6). Mol Pain. 2012; 8: 6. CrossRef PubMed PubMedCentral
  17.  
  18. Ku WH, Schneider SP. Multiple T-type Ca2+ current subtypes in electrophysiologically characterized hamster dorsal horn neurons: possible role in spinal sensory integration. J Neurophysiol. 2011; 106 (5): 2486-98. CrossRef PubMed PubMedCentral
  19.  
  20. Miao X, Meng X, Wu G, Ju Z, Zhang HH, Hu S, Xu GY. Upregulation of cystathionine-beta-synthetase expression contributes to inflammatory pain in rat temporomandibular joint. Mol Pain. 2014; 10 (1): 9.
  21.  
  22. Locatelli F, Botta L, Prestori F, Masetto S, D'Angelo E. Late-onset bursts evoked by mossy fibre bundle stimulation in unipolar brush cells: evidence for the involvement of H- and TRP-currents. J Physiol. 2013; 591 (Pt 4): 899-918.
  23.  
  24. Cains S, Blomeley CP, Bracci E. Serotonin inhibits lowthreshold spike interneurons in the striatum. J Physiol. 2012; 590 (Pt 10): 2241-52.
  25.  
  26. Chen QH, Andrade MA, Calderon AS, Toney GM. Hypertension induced by angiotensin II and a high salt diet involves reduced SK current and increased excitability of RVLM projecting PVN neurons. J Neurophysiol. 2010; 104 (5): 2329-37. CrossRef PubMed PubMedCentral
  27.  
  28. Zhao ML, Wu CF. Alterations in frequency coding and activity dependence of excitability in cultured neurons of Drosophila memory mutants. J Neurosci. 1997; 17 (6): 2187-99.
  29.  
  30. Druckmann S, Berger TK, Schurmann F, Hill S, Markram H, Segev I. Effective stimuli for constructing reliable neuron models. PLoS Comput Biol. 2011; 7 (8): e1002133.
  31.  
  32. Jung SC, Hoffman DA. Biphasic somatic A-type K channel downregulation mediates intrinsic plasticity in hippocampal CA1 pyramidal neurons. PLoS One. 2009; 4 (8): e6549.
  33.  
  34. Chen QH, Toney GM. Excitability of paraventricular nucleus neurones that project to the rostral ventrolateral medulla is regulated by small-conductance Ca2+-activated K+ channels. J Physiol. 2009; 587 (Pt 17): 4235-47.
  35.  
  36. Huang J, Yang Y, Zhao P, Gerrits MM, Hoeijmakers JG, Bekelaar K, Merkies IS, Faber CG, Dib-Hajj SD, Waxman SG. Small-fiber neuropathy Nav1.8 mutation shifts activation to hyperpolarized potentials and increases excitability of dorsal root ganglion neurons. J Neurosci. 2013; 33 (35): 14087-97. CrossRef PubMed
  37.  
  38. Feng B, Strichartz G. Endothelin-1 raises excitability and reduces potassium currents in sensory neurons. Brain Res Bull. 2009; 79 (6): 345-50. CrossRef PubMed PubMedCentral
  39.  
  40. Lang PM, Hilmer VB, Grafe P. Differential contribution of sodium channel subtypes to action potential generation in unmyelinated human C-type nerve fibers. Anesthesiology. 2007; 107 (3): 495-501. CrossRef PubMed
  41.  
  42. Grundken C, Hanske J, Wengel S, Reuter W, Abdulazim A, Shestopalov VI, Dermietzel R, Zoidl G, Prochnow N. Unified patch clamp protocol for the characterization of Pannexin 1 channels in isolated cells and acute brain slices. J Neurosci Methods. 2011; 199 (1): 15-25. CrossRef PubMed
  43.  
  44. Yavorskii VA, Lukyanetz EA. Evoked Impulse Activity of Isolated Hippocampal Neurons in the Perforated PatchClamp Configuration. Neurophysiology. 2012; 43: 417-24. CrossRef  
  45. Yavorsky VA, Lukyanetz EA. Using serial tachograms to measure the evoked impulse activity of isolated hippocampal neurons. Fiziol Zh. 2013; 59 (5): 3-10. [Ukrainian] CrossRef  
  46. Yavorskii VA, Kostyuk PG, Lukyanetz EA. Accommodation properties of isolated hippocampal neurons under conditions of an experimental model of epilepsy. Neurophysiology. 2006; 38: 175-81. CrossRef  
  47. Yavorskii VA, Lukyanetz EA. Effect of lithium ions on the accommodation characteristics of neurons from the CA1 hippocampal zone. Neurophysiology. 2000; 32: 220-2. CrossRef  
  48. Yavorsky VA, Lukyanetz EA. The role of Ca ions in frequency accommodation in rat hippocampal neurones. Ukr Biokhim Zh. 2001; 73: 127. [Ukrainian]
  49.  
  50. Yavorsky VA, Lukyanetz EA. Interspike model of neuronal impulse activity. Fiziol Zh. 2009; 55: 135. [Ukrainian].CrossRef  
  51. Yavorsky VA, Lukyanetz EA. Pilocarpine-induced epileptiform activity of isolated CA1 hippocampal neurons. Neurophysiology. 1997; 29: 162-7. CrossRef  

© National Academy of Sciences of Ukraine, Bogomoletz Institute of Physiology, 2014-2019.