THE EFFECT OF CHRONIC INTOXICATION WITH LOW DOSES OF CHLORPYRIFOS ON THE BEHAVIORAL PARAMETERS OF FEMALE RATS
S.V. Grabovska, Y.T. Salyha
Instityte of Animal Biology National Academy of Agrarian Scince of Ukraine, Lviv
We explored the effect of chronic chlorpyrifos intake on the
behavior of female laboratory rats. The animals were exposed
to chlorpyrifos in doses 5 and 10 mg/kg daily for a month long
and tested with behavioral tests: Morris water maze, Open
field test, and Dark/light box. Everyday chlorpyrifos intake
in dose 10 mg/kg was revealed to cause long- and short-term
spatial memory lesion, decrease in total activity, sharp changes
in the level of anxiety (it significantly increased at the start
of xenobiotic intake, and then normalized with time). Similar
trends, though less intense, were observed in the group exposed
to 5 mg/kg chlorpyrifos. From the survey results it can
be concluded that long-term (30 days long) intake of even
low doses of chlorpyrifos can cause adverse effects on the
functional state of nervous system.
chlorpyrifos; organophosphates; rats; behavior; memory; Open field; Morris water maze; Light/Dark box.
- Salyha Y. Biological effects assessment of chlorpyrifos and some aspects of its neurotoxicity. Visnyk of Lviv University. Biology series. 2010; 54:3–14.
- Salyha Y. Chlorpyrifos leads to oxidative stress-induced death of hippocampal cells in vitro. Neurophysiology. 2013; 45(3):193-9.
- Slotkin TA, Seidler FJ. Comparative developmental neurotoxicity of organophosphates in vivo: transcriptional responses of pathways for brain cell development, cell signaling, cytotoxicity and neurotransmitter systems. Brain Res Bull. 2007; 72(4/6):232-74.
- Rauh V, Arunajadai S, Horton M, Perera F, Hoepner L, Barr DB, Whyatt R. Seven-year neurodevelopmental scores and prenatal exposure to chlorpyrifos, a common agricultural pesticide. Environ Health Perspect. 2011; 8(119):1196-201.
- Brown TP, Rumsby PC, Capleton AC, Rushton L, Levy LS. Resticides and parkinson's disease – is there a link? Environ Health Perspect. 2006; 2(114):156–64.
- Shelton JF, Hertz-Picciotto I, Pessah IN. Tipping the balance of autism risk: potential mechanisms linking pesticides and autism. Environ Health Perspect. 2012; 7(120):944–51.
- Richendrfer H, Pelkowski SD, Colwill RM, Créton R. Developmental sub-chronic exposure to chlorpyrifos reduces anxiety-related behavior in zebrafish larvae. Neurotoxicol Teratol. 2012; 4(34):458-65.
- Ricceri L, Venerosi A, Capone F, Cometa MF, Lorenzini P, Fortuna S, Calamandrei G. Developmental neurotoxicity of organophosphorous pesticides: fetal and neonatal exposure to chlorpyrifos alters sex-specific behaviors at adulthood in mice. Toxicol Sci. 2006; 1(93):105-13.
- Braquenier JB, Quertemont E, Tirelli E, Plumier JC. Anxiety in adult female mice following perinatal exposure to chlorpyrifos. Neurotoxicol Teratol. 2010; 2(32):234-9.
- Mullen BR, Khialeeva E, Hoffman DB. Decreased reelin expression and organophosphate pesticide exposure alters mouse behavior and brain morphology. ASN Neuro. 2013; 5(1):27-42.
- Aldridge JE, Levin ED, Seidler FJ, Slotkin TA. Developmental exposure of rats to chlorpyrifos leads to behavioral alterations in adulthood, involving serotonergic mechanisms and resembling animal models of depression. Environ Health Perspect. 2005; 5(113):527-531.
- Terry AV Jr, Beck W., Warner S, Vandenhuerk L., Callahan PM. Chronic impairments in spatial learning and memory in rats previously exposed to chlorpyrfos or diisopropylfluorophosphate. Neurotoxicol Teratol. 2012; 1(34):1-8.
- Yan C, Jiao L, Zhao J, Yang H, Peng S. Repeated exposures to chlorpyrifos lead to spatial memory retrieval impairment and motor activity alteration. Neurotoxicol Teratol. 2012; 4(34):442-9.
- European convention for the protection of vertebrate animals used for experim. and other scientific purposes. Coun. of Europe, Strasbourg; 1986.
- Bourin M., Hascoe M. The mouse light/dark box test. Eur J Pharmacol. 2003; 463:55-65.
- Hall CS, Ballachey EL. A study of the rat's behavior in a field: a contribution to method in comparative psychology. University of California Publications in Psychology. 1932; 6:1–12.
- Morris RGM, Garrud P, Rawlins PNJ, O'Keefe J. Place navigation impaired in rats with hippocampal lesions. Nature. 1982; 297:681-3.
- Acharjee S, Nayani N, Tsutsui M, Hill MN, Ousman SS, Pittman QJ. Altered cognitive-emotional behavior in early experimental autoimmune encephalitis. Cytokine and hormonal correlates. Brain Behav. Immun. 2013 Oct; 33:164–72.
- Liu CL, Chen H, Jiang Y, Tu PF, Zhong M, Ma J., Ding H,. Zhang WX, Jin XM. Effects of echinacoside on extracellular acetylcholine and choline levels of hippocampus and striatum of cerebral ischemia rats. Yao Xue Xue Bao. 2013; 5(48):790-3.
- Zheng SQ, An LX, Cheng X, Wang YJ. Sevoflurane causes neuronal apoptosis and adaptability changes of neonatal rats. Acta Anaesthesiol Scand. 2013; 57(9):1167–1174.
- Davis S, Butcher SP, Morris RG. The NMDA receptor antagonist D-2-amino-5-phosphonopentanoate (D-AP5) impairs spatial learning and LTP in vivo at intracerebral concentrations comparable to those that block LTP in vitro. J Neurosci. 1992; 1(12):21–34.
- Chaouloff F, Durand M, Mormède P. Anxiety- and activityrelated effects of diazepam and chlordiazepoxide in the rat light/dark and dark/light tests. Behav Brain Res. 1997; 1(85):27-35.
- Heijtza RD., Wangc Sh, Anuard F, Qiana Y, Björkholm B, Samuelsson A, Hibberc M L, Forssberg H, Pettersson S. Normal gut microbiota modulates brain development and behavior. Rochellys Diaz Heijtz. 2011; 108(7):3047–52.
- Kasimova SK. Anxiety-like behavior in rats and its dependency on the lighting mode. Mod probl sci ed. 2006; 2:45-7.
- Grabovskaya SV, Salyha YT. Do Results of the Open Field Test Depend on the Arena Shape? Neurophysiology. 2014; 46 (4):376-80.