Українська Русский English

ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2015; 61(2): 80-86


FULLERENE C[sub]60[/sub] INHIBITED FREE RADICAL AND DESTRUCTIVE PROCESSES IN CONNECTIVE TISSUE DURING ADJUVANT ARTHRITIS IN RATS

T. V. Mamontova, L. E. Vesnina, M. V. Mikityuk, N. A. Bobrova, L. A. Kutsenko, I. L. Gordinskaya, I.P. Kaidashev

    Research Institute for Genetic and Immunological Grounds of Patology and Pharmacogenetic, Ukrainian Medical Stomatological Academy, Poltava
DOI: https://doi.org/10.15407/fz61.02.080

Abstract

The effects of fullerene C60 (FС60) on the level of free radiсal and destruction processes were studied in rats with experimental adjuvant arthritis (AA). It was shown the protective effect of FС60 during AA. The effect was accompanied by an increase of the antioxidant enzymes activity, superoxidedismutase in the liver (15,96±0,38 µmol/kg·s) and in the kidneys (5,36±0,27 µmol/kg·s) and catalase in the kidneys (9,56±0,78 µmol/kg·s) and in the heart (2,26±0,41 µmol/kg·s) in comparison to control group (43,83±5,69 %; 54,55±6,18%; 11,68±0,52 µmol/kg·s; 3,43±0,47 µmol/kg·s; 4,77±0,5 µmol/kg·s; 0,98±0,12 µmol/kg·s accordingly). It was shown a protective effect of FС60 during AA directed on the depression of the destructive processes in connective tissue that was expressed through the reduction of the total collagenolitic activity level in cartilage (10,05 ± 0,06 µmol/g/min) and bone (11,21±0,04 µmol/g/min) tissues, free hydroxyproline contents (1,54±0,04 µg/ml) and alkaline phasphatase activity (1,24±0,14 µmol/l·sec) in comparison to control group (11,91 ± 0,49 µmol/g/min; 13,19±0,15 µmol/g/min; 2,25±0,07 µg/ ml; 2,19±0,24 µmol/l·sec accordingly). Taken together, these results accentuate the perspective of future investigations of action FС60 during rheumatoid arthritis as a feasible therapeutic agent.

Keywords: experimental adjuvant arthritis; fullerene C60; antioxidant enzymes activity; total collagenolitic activity.

References

  1. Bendele A M. Animal models of rheumatoid arthritis. J Musculoskel Neuron Interact. 2001 1; 4: 377-85.
  2.  
  3. Vasiliauskas A, Leonaviciene L, Vaitkiene D, Bradunaite R, Luksiene A. Anti-inflammatory effect of Aesculus hippocastanum L. tincture and pro-/antioxidant bodily state of rats with adjuvant arthritis. Acta Medica Lituan. 2010 17; 3: 123-32.
  4.  
  5. Ma H L, Liang H J. Fullerenes as unique nanopharmaceuticals for disease treatment. Sci China Chem. 2010 53; 11: 2233-40.
  6.  
  7. Kraszewski S, Tarek M, Ramseyer C. Uptake and translocation mechanisms of cationic amino derivatives functionalized on pristine C60 by lipid membranes: a molecular dynamics simulation study. ACS Nano. 2011 5; 11: 8571–8.
  8.  
  9. Kutsenko N L, Mikitiuk M V, Bobrova N A, Kaĭdashev I P. Effect of fullerenes on the allergic inflammation development in experiment. Med Ecol Probl. 2009 13; 5-6: 7-12. [Ukrainian].
  10.  
  11. Ji H, Yang Z, Jiang W, Geng C, Gong M, Xiao H, et al. Antiviral activity of nanocarbone fullerene lipidosome against influenza virus in vitro. J Huazhong Univ Sci Technolog Med Sci. 2008 28; 3: 243-6.
  12.  
  13. Zogovic N S, Nikolic N S, Vranjes-Djuric S D, Harhaji L M, Vucicevic L M, Janjetovic K D, et al. Opposite effects of nanocrystalline fullerene (C(60)) on tumour cell growth in vitro and in vivo and a possible role of immunosupression in the cancer-promoting activity of C(60). Biomat. 2009 30; 36: 6940-6.
  14.  
  15. Lyon D Y, Adams L K, Falkner J C, Alvarez P J J. Antibacterial activity of fullerene C60 water suspensions: effects of preparation method and particle size. Environ Sci Technol. 2006 40; 14: 4360–6.
  16.  
  17. Vesnina L E, Mamontova T V, Mikituk M V, Bobrova N A, Kaidashev I P Stimulatory effect of fullerene С60 on production of hemagglutinins and hemolysins, level activity of complement during the primary immune response in Balb/c mice. Allergol Immunol. 2011 12; 4: 342-6. [Russian].
  18.  
  19. Vesnina L É, Mamontova T V, Mikitiuk M V, Bobrova N A, Kutsenko LA, Iaroshenko GA, Kaĭdashev IP. Fullerene C60 exhibits immunomodulatory activity during adjuvantinduced arthritis in rats. Eksp Klin Farmakol. 2012 75; 8:15-20. [Russian].
  20.  
  21. Tsuchiya T, Yamakoshi Y N, Miyata N A. A novel promoting action of fullerene C60 on the chondrogenesis in rat embryonic limb bud cell culture system. Biochem Biophys Res Commun. 1995 206: 885-94.
  22.  
  23. Gharbi N, Pressac M, Hadchouel M, Hadchouel M, Szwarc H, Wilson SR, Moussa F, et al. [60] Fullerene is a powerful antioxidant in vivo with no acute or subacute toxicity. Nano Lett. 2005 5; 12: 2578-85.
  24.  
  25. Berkalo L V, Bobovich О V, Bobrova N A, et al Kaĭdashev I P, editor. Methods of clinical and experimental investigations in medicine. Poltava: Polimet, 2003. Ukrainian.
  26.  
  27. Rayan J J, Bateman H R, Stover A, Gomez G, Norton S K, Zhao W, et al. Fulleren nanomaterials inhibit the allergic response. J Immunol. 2007 179: 665-72.
  28.  
  29. Dhawan A, Taurozzi J S, Pandey A K, Shan W, Miller S M, Hashsham S A, et al. Stable colloidal dispersion of C60 fullerenes in water: evidence for genotoxicity. Environ Sci Technol. 2006 40: 7394-401.
  30.  
  31. Tsuji G, Koshiba M, Nakamura H, Kosaka H, Hatachi S, Kurimoto C, et al. Thioredoxin protects against joint destruction in a murine arthritis model. Free Radic Biol Med. 2006 40; 10: 1721-31.
  32.  
  33. Ozkan Y, Yardim-Akaydyn S, Sepici A, Keskin E, Sepici V, Simsek B. Oxidative status in rheumatoid arthritis. Clin Rheumatol. 2007 26; 1: 64-8.
  34.  
  35. Yudoh K, Karasawa R, Masuko K, Kato T. Water-soluble fullerene (C60) inhibits the osteoclast differentiation and bone destruction in arthritis. Int J Nanomed. 2009 4: 233-9.
  36.  
  37. Romas E, Gillespie M T, Martin T J. Involvement of receptor activator of NFkappaB ligand and tumor necrosis factor-alpha in bone destruction in rheumatoid arthritis. Bone. 2002 30; 2: 340-6.
  38.  
  39. Leonaviciene L, Bernotiene E, Bradunaite R, Vaitkienл, Redaitienл E, Astrauskas V. Antiarthritic and hepatoprotective affect of derinat on adjuvant arthritis in rats Acta Medica Lituan. 2006 13; 4: 236-44.
  40.  
  41. Trnavska Z, Grimova J, Trnavsky K. Collagene metabolism in adjuvant-induced arthritis in the rat. Ann Rheum Dis. 1972 31: 334-8.
  42.  
  43. Simsek B, Karacaer O, Karaca I. Urine products of bone breakdown as markers of bone resorption and clinical usefulness of hydroxyproline: an overview. Clin Med J. 2004 117; 2: 291-5.
  44.  
  45. Borashan F A, Ilkhanipoor M, Farrokhi M H F. Investigation the effects of Curcumin on serum hepatic enzymes activity in a rheumatoid arthritis model. Electronic J Biol. 2009 4; 4: 129-33.
  46.  
  47. Morgan S L, Chen D-T, Carlee J, Baggott J E. Effect of methotrexate therapy on bone mineral density and body composition in rat adjuvant arthritis. J Rheumatol. 2004 31; 9: 1693-7.
  48.  

© National Academy of Sciences of Ukraine, Bogomoletz Institute of Physiology, 2014-2019.