DIACYLGLYCEROL ACCUMULATION IMPAIRS SHORT-TERM ACTIVATION OF PHOSPHOLIPASE D BY THYROXINE IN THE LIVER CELLS
Loay Kh.M. Hassouneh
- Faculty of Pharmacy, Al-Isra University, P.O. Box 22,
Amman, 11622, Jordan;
- Department of Physiology of Ontogenesis, Institute of
Biology, Kharkov Karazin National University, 4 Svobody
pl., 61022,Kharkov, Ukraine
Thyroid hormones (TG) are known modulators of signal
transduction. Phospholipase D (PLD) is one of the targets
of TG in the stimulated cells. Response of cells to the shortterm
TG action significantly reduces at old age. Taking into
account that diacylglycerol (DAG) accumulation induces the
resistance of cells to some of regulatory factors in the target
cells the aim of the present study was to determine if DAG
content increase in hepatocytes impairs the L-thyroxine (L-T4
short-term action. The experiments were performed in either
С]palmitic acid- labeled hepatocytes or [ 14
liver cells of 3- and 24-month-old rats. To study
the short-term L-T4
action on cells the PLD activation was
determined. The DAG production and content in hepatocytes
significantly increased at old age and in the young cells pretreated
with palmitic acid. The reduction of DAG level in cells
by means of DAG-kinase activator, alfa-tocoferol acetate, or
treatment improved the short-term hormone
action. The above data have indicated that DAG play important
role in the L-T4
PLD regulation. The cross-talk between
classic and non-genomic pathways of TG regulation of lipid
metabolism has been determined.
aging, hepatocytes, palmitic acid, diacylglycerol, thyroxine, phospholipase D.
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