Українська Русский English

ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2015; 61(2): 73-79


DIACYLGLYCEROL ACCUMULATION IMPAIRS SHORT-TERM ACTIVATION OF PHOSPHOLIPASE D BY THYROXINE IN THE LIVER CELLS

Loay Kh.M. Hassouneh

  1. Faculty of Pharmacy, Al-Isra University, P.O. Box 22, Amman, 11622, Jordan;
  2. Department of Physiology of Ontogenesis, Institute of Biology, Kharkov Karazin National University, 4 Svobody pl., 61022,Kharkov, Ukraine
DOI: https://doi.org/10.15407/fz61.02.073

Abstract

Thyroid hormones (TG) are known modulators of signal transduction. Phospholipase D (PLD) is one of the targets of TG in the stimulated cells. Response of cells to the shortterm TG action significantly reduces at old age. Taking into account that diacylglycerol (DAG) accumulation induces the resistance of cells to some of regulatory factors in the target cells the aim of the present study was to determine if DAG content increase in hepatocytes impairs the L-thyroxine (L-T4) short-term action. The experiments were performed in either the [14С]palmitic acid- labeled hepatocytes or [ 14 С]oleic acidpre-labeled liver cells of 3- and 24-month-old rats. To study the short-term L-T4 action on cells the PLD activation was determined. The DAG production and content in hepatocytes significantly increased at old age and in the young cells pretreated with palmitic acid. The reduction of DAG level in cells by means of DAG-kinase activator, alfa-tocoferol acetate, or long-term L-T4 treatment improved the short-term hormone action. The above data have indicated that DAG play important role in the L-T4 PLD regulation. The cross-talk between classic and non-genomic pathways of TG regulation of lipid metabolism has been determined.

Keywords: aging, hepatocytes, palmitic acid, diacylglycerol, thyroxine, phospholipase D.

References

  1. Gustavsson L, Moehern G, Torres-Marquez ME, Benistant C, Rubin R, Hock JB. The role of cytosolic Ca2+, protein kinase C and protein kinase A in hormonal stimulation of phospholipase D in rat hepatocytes. J Biol Chem. 1994; 269:849-859.
  2.  
  3. Pettitt TR, Martin A, Horton T, Liossis C, Lord JM, Wakelam MJO. Diacylglycerol and phosphatidate generated by phospholipase C and D, respectively, have distinct fatty acid compositions and function. Phospholipase Dderived diacylglycerol does not activated protein kinase C in porcine aortic endothelial cells. J Biol Chem. 1997; 15(5):574-84.
  4.  
  5. Jornayvaz FR, Shulman GI. Diacylglycerol activation of protein kinase Cε and hepatic insulin resistance. Cell Metab. 2012; 15(5):574-584. CrossRef PubMed PubMedCentral
  6.  
  7. Erion DM, Shulman GI. Diacylglycerol-mediated insulin resistance. Nat Med. 2010; 16(4):400-402. CrossRef PubMed PubMedCentral
  8.  
  9. Kavok NS, Krasilnikova OA, Babenko NA. Thyroxine signal transduction in liver cells involves phospholipase C and phospholipase D activation. Genomic independent action of thyroid hormone. BMC Cell Biol [Internet]. 2001 Apr. 2(5). Available from: http:www.biomedcentral. com/1471-2121/2/5.
  10.  
  11. Krasilnikova OA, Kavok NS, Babenko NA. Drug-induced and postnatal hypothyroidism impairs the accumulation of diacylglycerol inliver and liver cell plasma membranes. BMC Physiol [Internet]. 2002 Aug. 2(12). Available from: http:www.biomedcentral.com/1472-6793/2/12.
  12.  
  13. Petrenko AIu, Sukach AN, Rosliakov AD. Isolation of rat hepatocytes by a nonenzymatic method: detoxifying and respiratory activity. Biokhimiia. 1991; 56(9):1647- 51 (Russian).
  14.  
  15. Bligh EG, Dyer WJ. A rapid method of total lipid extraction and purification. Can J Biochem Physiol. 1959; 37(8):911-917. CrossRef PubMed
  16.  
  17. Carrasco S, Merida I. Diacylglycerol, when simplicity becomes complex. Trends Biochem Sci. 2007; 32(1):27-36. CrossRef PubMed
  18.  
  19. Yang C, Kazanietz MG. Divergence and complexities in DAG signaling: looking beyond PKC. Trends Pharmacol. Sci. 2003; 24(11):602-608.
  20.  
  21. Brose N, Betz A, Wegmeyer H. Divergent and convergent signaling by the diacylglycerol second messenger pathway in mammals. Curr Opin Neurobiol. 2004; 14(3):328-340. CrossRef PubMed
  22.  
  23. Kavok NS, Krasilnikova OA, Babenko NA. Increase in diacylglycerol production by liver and liver cell nuclei at old age. Exp Gerontol. 2003; 38:441-447. CrossRef  
  24. Babenko NA. Effects of short- and long-term saturated fat-enriched diet on the ceramide and neutral lipids accumulation in the insulin responsive tissues of rats. In: Langella JP, editor. Saturated fats: Metabolism, Disease Risks and Public Awareness. New York: Nova Science Publishers; 2012. 71-97.
  25.  
  26. Girard J, Perdereau D, Foufelle F, Prip-Buus C, Ferre P. Regulation of lipogenic enzyme gene expression by nutrients and hormones. FASEB J. 1994; 8(1):36-42.
  27.  
  28. Davis PJ, Leonard JL, Davis FB. Mechanisms of nongenomic actions of thyroid hormone. Front Neuroendocrinol. 2008; 29:211-218. CrossRef PubMed
  29.  
  30. Cordeiro A, Souza LL, Einicker-Lamas M, Pazos-Moura CC. Non-classic thyroid hormone signalling involved in hepatic lipid metabolism. J Endocrinol. 2013 Feb 25; 216(3):R47 - R57.
  31.  
  32. Krasilnikova OA, Kavok NS, Babenko NA. Role of calcium ions in rapid effects of L-thyroxine on phosphoinositide metabolism in rat liver cells. Biochemistry (Moscow). 2003; 68(7):776-782. CrossRef

© National Academy of Sciences of Ukraine, Bogomoletz Institute of Physiology, 2014-2019.