|
|
|
|
DIACYLGLYCEROL ACCUMULATION IMPAIRS SHORT-TERM ACTIVATION OF PHOSPHOLIPASE D BY THYROXINE IN THE LIVER CELLS
Loay Kh.M. Hassouneh
- Faculty of Pharmacy, Al-Isra University, P.O. Box 22,
Amman, 11622, Jordan;
- Department of Physiology of Ontogenesis, Institute of
Biology, Kharkov Karazin National University, 4 Svobody
pl., 61022,Kharkov, Ukraine
DOI: https://doi.org/10.15407/fz61.02.073
![](/images/cite.png)
Abstract
Thyroid hormones (TG) are known modulators of signal
transduction. Phospholipase D (PLD) is one of the targets
of TG in the stimulated cells. Response of cells to the shortterm
TG action significantly reduces at old age. Taking into
account that diacylglycerol (DAG) accumulation induces the
resistance of cells to some of regulatory factors in the target
cells the aim of the present study was to determine if DAG
content increase in hepatocytes impairs the L-thyroxine (L-T 4)
short-term action. The experiments were performed in either
the [ 14С]palmitic acid- labeled hepatocytes or [ 14 С]oleic acidpre-labeled
liver cells of 3- and 24-month-old rats. To study
the short-term L-T 4 action on cells the PLD activation was
determined. The DAG production and content in hepatocytes
significantly increased at old age and in the young cells pretreated
with palmitic acid. The reduction of DAG level in cells
by means of DAG-kinase activator, alfa-tocoferol acetate, or
long-term L-T 4 treatment improved the short-term hormone
action. The above data have indicated that DAG play important
role in the L-T 4 PLD regulation. The cross-talk between
classic and non-genomic pathways of TG regulation of lipid
metabolism has been determined.
Keywords:
aging, hepatocytes, palmitic acid, diacylglycerol, thyroxine, phospholipase D.
References
- Gustavsson L, Moehern G, Torres-Marquez ME, Benistant C, Rubin R, Hock JB. The role of cytosolic Ca2+, protein kinase C and protein kinase A in hormonal stimulation of phospholipase D in rat hepatocytes. J Biol Chem. 1994; 269:849-859.
- Pettitt TR, Martin A, Horton T, Liossis C, Lord JM, Wakelam MJO. Diacylglycerol and phosphatidate generated by phospholipase C and D, respectively, have distinct fatty acid compositions and function. Phospholipase Dderived diacylglycerol does not activated protein kinase C in porcine aortic endothelial cells. J Biol Chem. 1997; 15(5):574-84.
- Jornayvaz FR, Shulman GI. Diacylglycerol activation of protein kinase Cε and hepatic insulin resistance. Cell Metab. 2012; 15(5):574-584.
CrossRef
PubMed PubMedCentral
- Erion DM, Shulman GI. Diacylglycerol-mediated insulin resistance. Nat Med. 2010; 16(4):400-402.
CrossRef
PubMed PubMedCentral
- Kavok NS, Krasilnikova OA, Babenko NA. Thyroxine signal transduction in liver cells involves phospholipase C and phospholipase D activation. Genomic independent action of thyroid hormone. BMC Cell Biol [Internet]. 2001 Apr. 2(5). Available from: http:www.biomedcentral. com/1471-2121/2/5.
- Krasilnikova OA, Kavok NS, Babenko NA. Drug-induced and postnatal hypothyroidism impairs the accumulation of diacylglycerol inliver and liver cell plasma membranes. BMC Physiol [Internet]. 2002 Aug. 2(12). Available from: http:www.biomedcentral.com/1472-6793/2/12.
- Petrenko AIu, Sukach AN, Rosliakov AD. Isolation of rat hepatocytes by a nonenzymatic method: detoxifying and respiratory activity. Biokhimiia. 1991; 56(9):1647- 51 (Russian).
- Bligh EG, Dyer WJ. A rapid method of total lipid extraction and purification. Can J Biochem Physiol. 1959; 37(8):911-917.
CrossRef
PubMed
- Carrasco S, Merida I. Diacylglycerol, when simplicity becomes complex. Trends Biochem Sci. 2007; 32(1):27-36.
CrossRef
PubMed
- Yang C, Kazanietz MG. Divergence and complexities in DAG signaling: looking beyond PKC. Trends Pharmacol. Sci. 2003; 24(11):602-608.
- Brose N, Betz A, Wegmeyer H. Divergent and convergent signaling by the diacylglycerol second messenger pathway in mammals. Curr Opin Neurobiol. 2004; 14(3):328-340.
CrossRef
PubMed
- Kavok NS, Krasilnikova OA, Babenko NA. Increase in diacylglycerol production by liver and liver cell nuclei at old age. Exp Gerontol. 2003; 38:441-447.
CrossRef
- Babenko NA. Effects of short- and long-term saturated fat-enriched diet on the ceramide and neutral lipids accumulation in the insulin responsive tissues of rats. In: Langella JP, editor. Saturated fats: Metabolism, Disease Risks and Public Awareness. New York: Nova Science Publishers; 2012. 71-97.
- Girard J, Perdereau D, Foufelle F, Prip-Buus C, Ferre P. Regulation of lipogenic enzyme gene expression by nutrients and hormones. FASEB J. 1994; 8(1):36-42.
- Davis PJ, Leonard JL, Davis FB. Mechanisms of nongenomic actions of thyroid hormone. Front Neuroendocrinol. 2008; 29:211-218.
CrossRef
PubMed
- Cordeiro A, Souza LL, Einicker-Lamas M, Pazos-Moura CC. Non-classic thyroid hormone signalling involved in hepatic lipid metabolism. J Endocrinol. 2013 Feb 25; 216(3):R47 - R57.
- Krasilnikova OA, Kavok NS, Babenko NA. Role of calcium ions in rapid effects of L-thyroxine on phosphoinositide metabolism in rat liver cells. Biochemistry (Moscow). 2003; 68(7):776-782.
CrossRef
|
|
|
|
|
|
|