Українська Русский English

ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2016; 62(1): 16-24


EFFECT OF TRPA1 RECEPTOR ACTIVATION ON TRPV1 CHANNEL DESENSITIZATION IN RAT DORSAL GANGLION NEURONS

A.V. Dragan1, O.A. Petrushenko1, O.P. Burlak1,2, E.A. Lukyanetz1,2

  1. Bogomoletz Institute of physiology NAS of Ukraine, Kyiv;
  2. International Center for Molecular Physiology NASU, Kyiv, Ukraine
DOI: https://doi.org/10.15407/fz62.01.016

Abstract

The activity of TRPA1 and TRPV1 channels, their sensitivity to selective activators - allyl isothiocyanate (AITC) and capsaicin (Caps), especially their interaction were studied. The method of microfluorescent microscopy and Ca2+ sensitive dye fura- 2AM. Registration of changes in the concentration of intracellular Ca2+ was performed by using the ratio of fluorescence signals measured at two wavelengths (R = F1/ F2). Researches were conducted on cultured neurons of rat dorsal ganglia (DRG neurons). Application of AITC and Caps on soma of DRG neurons resulted in an increase in intracellular Ca2+. Consistent repeated Caps applications resulted in a significant reduction in the amplitude of Ca2+ transients ( desensitization of TRPV1 channels), which accounted 20,7% of initial value. Further application of selective TRPA1 channel agonist (AITC) resulted in restoration of sensitivity to capsaicin TRPV1 channels ( resensitization TRPV1 channels). Thus, we have established the presence of regulation of TRPV1 channel activity by TRPA1 channels

Keywords: TRPV1-channels; TRPA1-receptors; calcium; DRG-neurons; calcium signaling; capsaicin; allyl isothiocyanate.

References

  1. Everaerts W, Gees M, Alpizar YA, Farre R, Leten C, Apetrei A. et al. The capsaicin receptor TRPV1 is a crucial mediator of the noxious effects of mustard oil. Curr Biol. 2011 Feb 22;21(4):316-21. CrossRef PubMed
  2.  
  3. Bevan S, Andersson DA. TRP channel antagonists for pain--opportunities beyond TRPV1. Curr Opin Investig Drugs. 2009 Jul;10(7):655-63. PubMed
  4.  
  5. Fernandes ES, Russell FA, Spina D, McDougall JJ, Graepel R, Gentry C, et al. A distinct role for transient receptor potential ankyrin 1, in addition to transient receptor potential vanilloid 1, in tumor necrosis factor alpha-induced inflammatory hyperalgesia and Freund's complete adjuvant-induced monarthritis. Arthritis Rheum. 2011 Mar;63(3):819-29. CrossRef PubMed
  6.  
  7. Fernandes ES, Fernandes MA, Keeble JE. The functions of TRPA1 and TRPV1: moving away from sensory nerves. Br J Pharmacol. 2012 May;166(2):510-21. CrossRef PubMed PubMedCentral
  8.  
  9. Brederson JD, Kym PR, Szallasi A. Targeting TRP channels for pain relief. Eur J Pharmacol. 2013 Sep 15;716(1-3):61-76. CrossRef PubMed
  10.  
  11. Hinman A, Chuang HH, Bautista DM, Julius D. TRP channel activation by reversible covalent modification. Proc Natl Acad Sci U S A. 2006 Dec 19;103(51):19564-8. CrossRef PubMed PubMedCentral
  12.  
  13. Gijsen HJ, Berthelot D, De Cleyn MA, Geuens I, Brone B, Mercken M. Tricyclic 3,4-dihydropyrimidine-2-thione derivatives as potent TRPA1 antagonists. Bioorg Med Chem Lett. 2012 Jan 15;22(2):797-800. CrossRef PubMed
  14.  
  15. Koplas PA, Rosenberg RL, Oxford GS. The role of calcium in the desensitization of capsaicin responses in rat dorsal root ganglion neurons. J Neurosci. 1997 May 15;17(10):3525-37. PubMed
  16.  
  17. Akopian AN, Ruparel NB, Jeske NA, Hargreaves KM. Transient receptor potential TRPA1 channel desensitization in sensory neurons is agonist dependent and regulated by TRPV1-directed internalization. J Physiol. 2007 Aug 15;583(Pt 1):175-93. CrossRef PubMed PubMedCentral
  18.  
  19. Vyklicky L, Novakova-Tousova K, Benedikt J, Samad A, Touska F, Vlachova V. Calcium-dependent desensitization of vanilloid receptor TRPV1: a mechanism possibly involved in analgesia induced by topical application of capsaicin. Physiol Res. 2008;57 Suppl 3:S59-S68. PubMed
  20.  
  21. Dragan A, Rosada V, Lukyanetz EA. Investigation of calcium signaling of TRPA1 membrane channels in the sensory neurons by fluorescent method. Proceedings of the XIIIth International Young Scientist's conference on applied physics. 2013: 142-3.
  22.  
  23. Kostyk PG, Kostyuk E, Lukyanetz EA. Calcium ions in brain function - from physiology to pathology. Kyiv: Naukova Dumka; 2005.[Ukrainian]
  24.  
  25. Kostyk PG, Kostyuk EP, Lukyanetz EA. Intracellular calcium signalling: structure and function. Kyiv: Naukova Dumka; 2009.
  26.  
  27. Staruschenko A, Jeske NA, Akopian AN. Contribution of TRPV1-TRPA1 interaction to the single channel properties of the TRPA1 channel. J Biol Chem. 2010 May 14;285(20):15167-77. CrossRef PubMed PubMedCentral
  28.  
  29. Lukyanetz EA, Stanika RI, Koval LM, Kostyuk PG. Intracellular mechanisms of hypoxia-induced calcium increase in rat sensory neurons. Arch Biochem Biophys. 2003;410(2):212-21. CrossRef  
  30. Jankowski MP, Ross JL, Weber JD, Lee FB, Shank AT, Hudgins RC. Age-dependent sensitization of cutaneous nociceptors during developmental inflammation. Mol Pain. 2014;10:34. CrossRef PubMed PubMedCentral
  31.  
  32. Lukyanetz IA, Kostyk PG, Lukyanetz EA. Calcium Signaling in Carassius Cerebellar Neurons: Role of the Mitochondria. Neurophysiology. 2009 Dec;41(6):375-9. CrossRef  
  33. Lukyanetz IA, Kostyk PG, Lukyanetz EA. The involvement of calcium transport systems of the plasma membrane in calcium exchange in neurons of the Carassius gibelio cerebellum. Neurophysiology. 2009 Aug;41(4):231-7. CrossRef  
  34. Lukyanets IA, Lukyanetz EA. Modulation of calcium signalling by the endoplasmic reticulum in Carassius neurons. Biochem Biophys Res Commun. 2013 Apr 19;433(4):591-4. CrossRef PubMed
  35.  
  36. Eid SR, Crown ED, Moore EL, Liang HA, Choong KC, Dima S, et al. HC-030031, a TRPA1 selective antagonist, attenuates inflammatory- and neuropathy-induced mechanical hypersensitivity. Mol Pain. 2008;4:48. CrossRef PubMed PubMedCentral
  37.  
  38. McNamara CR, Mandel-Brehm J, Bautista DM, Siemens J, Deranian KL, Zhao M, et al. TRPA1 mediates formalininduced pain. Proc Natl Acad Sci U S A. 2007 Aug 14;104(33):13525-30. CrossRef PubMed PubMedCentral
  39.  
  40. Akopian AN, Ruparel NB, Patwardhan A, Hargreaves KM. Cannabinoids desensitize capsaicin and mustard oil responses in sensory neurons via TRPA1 activation. J Neurosci. 2008 Jan 30;28(5):1064-75. CrossRef PubMed
  41.  
  42. Matta JA, Cornett PM, Miyares RL, Abe K, Sahibzada N, Ahern GP. General anesthetics activate a nociceptive ion channel to enhance pain and inflammation. Proc Natl Acad Sci U S A. 2008 Jun 24;105(25):8784-9. CrossRef PubMed PubMedCentral
  43.  
  44. Salas MM, Hargreaves KM, Akopian AN. TRPA1- mediated responses in trigeminal sensory neurons: interaction between TRPA1 and TRPV1. Eur J Neurosci. 2009 Apr;29(8):1568-78. CrossRef PubMed PubMedCentral
  45.  
  46. Vellani V, Mapplebeck S, Moriondo A, Davis JB, McNaughton PA. Protein kinase C activation potentiates gating of the vanilloid receptor VR1 by capsaicin, protons, heat and anandamide. J Physiol. 2001 Aug 1;534(Pt 3):813-25. CrossRef PubMed PubMedCentral
  47.  
  48. Fan HC, Zhang X, McNaughton PA. Activation of the TRPV4 ion channel is enhanced by phosphorylation. J Biol Chem. 2009 Oct 9;284(41):27884-91. CrossRef PubMed PubMedCentral
  49.  
  50. Studer M, McNaughton PA. Modulation of single-channel properties of TRPV1 by phosphorylation. J Physiol. 2010 Oct 1;588(Pt 19):3743-56. CrossRef PubMed PubMedCentral
  51.  
  52. Kostyuk PG, Lukyanetz EA, Ter-Markosyan AS. Parathyroid hormone enhances calcium current in snail neurones - Simulation of the effect by phorbol esters. Pflueg Arch. 1992;420(2):146-52. CrossRef  
  53. Kostyuk PG, Lukyanetz EA, Doroshenko PA. Effects of serotonin and cAMP on calcium currents in different neurones of Helix pomatia. Pflueg Arch /Eur J Physiol. 1992;420(1):9-15. CrossRef  
  54. Lukyanetz EA, Kostyuk PG. Two distinct receptors operate the cAMP cascade to up-regulate L-type Ca channels. Pflug Arch. 1996;432(2):174-81. CrossRef  
  55. Lukyanetz EA, Sotkis AV. Serotonin-induced changes in the activity of single Ca2+ channels inHelix pomatia neurons. Neurophysiology. 1996;28(2-3):103-10. CrossRef  
  56. Kostyuk PG, Lukyanetz EA. Mechanisms of antagonistic action of internal Ca2+ on serotonin-induced potentiation of Ca2+ currents in Helix neurones. Pflug Arch. 1993 Jun;424(1):73-83. CrossRef PubMed
  57.  
  58. Lukyanetz EA. Evidence for colocalization of calcineurin and calcium channels in dorsal root ganglion neurons. Neuroscience. 1997;78(3):625-8. CrossRef PubMed
  59.  
  60. Woo DH, Jung SJ, Zhu MH, Park CK, Kim YH, Oh SB, et al. Direct activation of transient receptor potential vanilloid 1(TRPV1) by diacylglycerol (DAG). Mol Pain. 2008;4:42. CrossRef PubMed PubMedCentral

© National Academy of Sciences of Ukraine, Bogomoletz Institute of Physiology, 2014-2019.