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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2016; 62(1): 107-113


CUMULUS CELL GENES AS POTENTIAL BIOMARKERS OF OOCYTE AND EMBRYO DEVELOPMENTAL COMPETENCE

E.А. Shepel, Т.Yu. Voznesenskaya, T.V. Blashkiv, R.І. Yanchii

    Bogomoletz Institute of physiology NAS of Ukraine, Kyiv;
DOI: https://doi.org/10.15407/fz62.01.107

Abstract

The selection of embryos with high implantation potential is the most important task in assisted reproductive technologies. Today, this selection is based on subjective morphological criteria such as growth rate, early cleavage, the degree of fragmentation, blastocyst formation. However, the morphological assessment alone does not accurately predict oocyte/early stage embryo competence. Thus, the development of an objective, accurate, fast and affordable tests to determine oocyte quality and embryo viability could increase the chance of a successful pregnancy and reduce the number of embryos to transfer. The advent of new technologies, the so-called OMIKS, has allowed to identify novel biomarkers that can be used in cycle of in vitro fertilization (IVF) for oocyte and / or embryo selection. During folliculogenesis oocyte plays a dominant role in regulation of cumulus (CC) and granulosa cell (GC) functions, and it is consequently believed that functions of GC and CC indirectly reflect oocyte’s competence. Cell functions and active cell processes are regulated through gene expression therefore, gene expression analysis in GC and/or CC could provide a non-invasive method for identification of the most competent oocytes and embryos.  In cumulus cells, genes have been identified that characterize the oocyte ability to undergo meiotic maturation, successful fertilization and early embryonic development. Among them cyclooxygenase 2, gremlin 1 and hyaluronan synthase-2, which play an important roles during oocyte development, ovulation and fertilization. This article reviews the recent data regarding these genes as potential biomarkers for selection of oocytes and embryos in the IVF program.

Keywords: oocyte quality; cumulus cell gene expression; cyclooxygenase 2; gremlin 1; hyaluronic acid synthase 2.

References

  1. Assou S, Haouzi D, De Vos J, Hamamah S. Human cumulus cells as biomarkers for embryo and pregnancy outcomes. Mol Hum Reprod. 2010 Aug;16(8):531-8. CrossRef PubMed
  2.  
  3. Fauser BC, Diedrich K, Bouchard P, Domínguez F, Matzuk M, Franks S, Hamamah S, Simón C,Devroey P, Ezcurra D, Howles CM. Contemporary genetic technologies and female reproduction. Hum Reprod Update. 2011 NovDec;17(6):829-47.
  4.  
  5. McKenzie LJ, Pangas SA, Carson SA, Kovanci E, Cisneros P, Buster JE,Amato P, Matzuk MM. Human cumulus granulosa cell gene expression: a predictor of fertilization and embryo selection in women undergoing IVF. Hum Reprod. 2004 Dec;19(12):2869-74. CrossRef PubMed
  6.  
  7. Fragouli E, Lalioti MD, Wells D. The transcriptome of follicular cells: biological insights and clinical implications for the treatment of infertility. Hum Reprod Update. 2014 Jan-Feb;20(1):1-11. CrossRef PubMed PubMedCentral
  8.  
  9. Assidi M, Montag M, Sirard MA. Use of both cumulus cells' transcriptomic markers and zona pellucida birefringence to select developmentally competent oocytes in human assisted reproductive technologies. BMC Genomics. 2015;16 Suppl 1:S9. CrossRef PubMed PubMedCentral
  10.  
  11. Uyar A, Torrealday S, Seli E. Cumulus and granulosa cell markers of oocyte and embryo quality. Fertil Steril. 2013 Mar 15;99(4):979-97. CrossRef PubMed PubMedCentral
  12.  
  13. Al-Shukri M, Khan D, Al-Hadrami A, Al-Riyami N, Gowri V, Haddabi R, Abdellatif M, Al-Dughaishi T. Maternal and fetal outcomes of triplet gestation in a tertiary hospital in oman. Sultan Qaboos Univ Med J. 2014 May;14(2):e204-10. PubMed PubMedCentral
  14.  
  15. Burnik Papler T, Vrtacnik Bokal E, Lovrecic L, Kopitar AN, Maver A. No specific gene expression signature in human granulosa and cumulus cells for prediction of oocyte fertilisation and embryo implantation. PLoS One. 2015 Mar 13;10(3):e0115865. CrossRef PubMed PubMedCentral
  16.  
  17. Assou S, Al-edani T, Haouzi D, Philippe N, Lecellier CH, Piquemal D, Commes T, Aït-Ahmed O, Dechaud H, Hamamah S. MicroRNAs: new candidates for the regulation of the human cumulus-oocyte complex. Hum Reprod. 2013 Nov;28(11):3038-49. CrossRef PubMed
  18.  
  19. Huang X, Hao C, Shen X, Liu X, Shan Y, Zhang Y, Chen L. Differences in the transcriptional profiles of human cumulus cells isolated from MI and MII oocytes of patients with polycystic ovary syndrome. Reproduction. 2013 May 21;145(6):597-608. CrossRef PubMed
  20.  
  21. Moussaddykine S, Assou S, Dechaud H, Hamamah S. Other actors in the oocyte and follicular growth: the role of microRNAs in the cumulus-oocyte dialog. Gynecol Obstet Fertil. 2012 Mar;40(3):170-3. CrossRef PubMed
  22.  
  23. Haouzi D, Assou S, Monzo C, Vincens C, Dechaud H, Hamamah S. Altered gene expression profile in cumulus cells of mature MII oocytes from patients with polycystic ovary syndrome. Hum Reprod. 2012 Dec;27(12):3523-30. CrossRef PubMed
  24.  
  25. Iager AE, Kocabas AM, Otu HH, Ruppel P, Langerveld A, Schnarr P, Suarez M, Jarrett JC, Conaghan J, Rosa GJ, Fernández E, Rawlins RG, Cibelli JB, Crosby JA. Identification of a novel gene set in human cumulus cells predictive of an oocyte's pregnancy potential. Fertil Steril. 2013 Mar 1;99(3):745-752. CrossRef PubMed
  26.  
  27. Jindal S, Greenseid K, Berger D, Santoro N, Pal L. Impaired gremlin 1 (GREM1) expression in cumulus cells in young women with diminished ovarian reserve (DOR). J Assist Reprod Genet. 2012 Feb;29(2):159-62. CrossRef PubMed PubMedCentral
  28.  
  29. Cillo F, Brevini TA,Antonini S, Paffoni A, Ragni G, Gandolfi F. Association between human oocyte developmental competence and expression levels of some cumulus genes. Reproduction. 2007 Nov;134(5):645-50. CrossRef PubMed
  30.  
  31. Li Y, Li RQ, Ou SB, Zhang NF, Ren L, Wei LN, Zhang QX,Yang DZ. Increased GDF9 and BMP15 mRNA levels in cumulus granulosa cells correlate with oocyte maturation, fertilization, and embryo quality in humans. Reprod Biol Endocrinol. 2014 Aug 20;12:81. CrossRef PubMed PubMedCentral
  32.  
  33. Du M, Fu X, Zhou Y, Zhu S. Effects of trichostatin A on cumulus expansion during mouse oocyte maturation. Asian-Australas J Anim Sci. 2013 Nov;26(11):1545-52. CrossRef PubMed PubMedCentral
  34.  
  35. Blaha M, Nemcova L, Kepkova KV, Vodicka P, Prochazka R. Gene expression analysis of pig cumulus-oocyte complexes stimulated in vitro with follicle stimulating hormone or epidermal growth factor-like peptides. Reprod Biol Endocrinol. 2015 Oct 6;13:113. CrossRef PubMed PubMedCentral
  36.  
  37. Wissing ML, Kristensen SG, Andersen CY, Mikkelsen AL, Host T, Borup R, Grondahl ML. Identification of new ovulation-related genes in humans by comparing the transcriptome of granulosa cells before and after ovulation triggering in the same controlled ovarian stimulation cycle. Hum Reprod. 2014 May;29(5):997-1010. CrossRef PubMed
  38.  
  39. Lin ZL, Li YH, Xu YN, Wang QL, Namgoong S, Cui XS, Kim NH. Effects of growth differentiation factor 9 Гени кумулюсних клітин як потенційні біомаркери для діагностики якості ооцитів та ембріонів ISSN 0201-8489 Фізіол. журн., 2016, Т. 62, № 1 113 and bone morphogenetic protein 15 on the in vitro maturation of porcineoocytes. Reprod Domest Anim. 2014 Apr;49(2):219-27.
  40.  
  41. Gui LM, Joyce IM. RNA interference evidence that growth differentiation factor-9 mediates oocyte regulation of cumulus expansion in mice. Biol Reprod. 2005 Jan;72(1):195-9. CrossRef PubMed
  42.  
  43. Vigone G, Merico V, Prigione A, Mulas F, Sacchi L, Gabetta M, Bellazzi R, Redi CA, Mazzini G, Adjaye J, Garagna S, Zuccotti M. Transcriptome based identification of mouse cumulus cell markers that predict the developmental competence of their enclosed antral oocytes. BMC Genomics. 2013 Jun 7;14:380. CrossRef PubMed PubMedCentral
  44.  
  45. Anderson RA, Sciorio R, Kinnell H, Bayne RA, Thong KJ, de Sousa PA, Pickering S. Cumulus gene expression as a predictor of human oocyte fertilisation, embryo development and competence to establish a pregnancy. Reproduction. 2009 Oct;138(4):629-37. CrossRef PubMed
  46.  
  47. Adriaenssens T, Wathlet S, Segers I, Verheyen G, De Vos A, Van der Elst J,Coucke W, Devroey P, Smitz J. Cumulus cell gene expression is associated with oocyte developmental quality and influenced by patient and treatment characteristics. Hum Reprod. 2010 May;25(5):1259-70. CrossRef PubMed
  48.  
  49. Assou S, Haouzi D, Dechaud H, Gala A, Ferrieres A, Hamamah S. Comparative gene expression profiling in human cumulus cells according to ovarian gonadotropin treatments. Biomed Res Int. 2013;2013:354582. CrossRef PubMed PubMedCentral
  50.  
  51. Ekart J, McNatty K, Hutton J, Pitman J. Ranking and selection of MII oocytes in human ICSI cycles using gene expression levels from associated cumulus cells. Hum Reprod. 2013 Nov;28(11):2930-42. CrossRef PubMed
  52.  
  53. Machado MF, Caixeta ES, Sudiman J, Gilchrist RB, Thompson JG, Lima PF, Price CA, Buratini J. Fibroblast growth factor 17 and bone morphogenetic protein 15 enhance cumulus expansion and improve quality of in vitro-produced embryos in cattle. Theriogenology. 2015 Aug;84(3):390-8. CrossRef PubMed
  54.  
  55. Gervasio CG, Bernuci MP, Silva-de-Sa MF, Rosa-ESilva AC. The role of androgen hormones in early follicular development. ISRN Obstet Gynecol. 2014 Apr 10;2014:818010.
  56.  
  57. Gershon E, Hourvitz A, Reikhav S, Maman E, Dekel N. Low expression of COX-2, reduced cumulus expansion, and impaired ovulation in SULT1E1-deficient mice. FASEB J. 2007 Jun;21(8):1893-901. CrossRef PubMed
  58.  
  59. Ochsner SA, Russell DL, Day AJ, Breyer RM, Richards JS. Decreased expression of tumor necrosis factoralpha-stimulated gene 6 in cumulus cells of the cyclooxygenase-2 and EP2 null mice. Endocrinology. 2003 Mar;144(3):1008-19. CrossRef PubMed
  60.  
  61. Richards JS. Ovulation: new factors that prepare the oocyte for fertilization. Mol Cell Endocrinol. 2005 Apr 29;234(1-2):75-9. CrossRef PubMed
  62.  
  63. Huang X, Hao C, Shen X, Zhang Y, Liu X. RUNX2, GPX3 and PTX3 gene expression profiling in cumulus cells are reflective oocyte/embryo competence and potentially reliable predictors of embryo developmental competence in PCOS patients. Reprod Biol Endocrinol. 2013 Nov 26;11:109. CrossRef PubMed PubMedCentral
  64.  
  65. Bunel A, Jorssen EP, Merckx E, Leroy JL, Bols PE, Sirard MA Individual bovine in vitro embryo production and cumulus cell transcriptomic analysis to distinguish cumulus-oocyte complexes with high or low developmental potential. Theriogenology. 2015 Jan 15;83(2):228-37. CrossRef PubMed

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