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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2015; 61(2): 48-59

Impact of C[sub]60[/sub] fullerene on the dynamics of force-speed changes in soleus muscle of rat at ischemia-reperfusion injury

D.M. Nozdrenko1, K.I. Bogutska1, Yu.I. Prylutskyy1, V.F. Korolovych1,2, M.P. Evstigneev3, U. Ritter4, P. Scharff4

  1. Taras Shevchenko National University of Kyiv, Kyiv, Ukraine
  2. Saratov State University, Saratov, Russia;
  3. Department of Biology and Chemistry, Belgorod State University, Belgorod, Russia;
  4. Technical University of Ilmenau, Institute of Chemistry and Biotechnology, Ilmenau, Germany


The effect of C60 fullerene nanoparticles (30-90 nm) on dynamics of force response development to stimulated soleus muscle of rat with ischemic pathology, existing in muscle during the first 5 hours and first 5 days after 2 hours of ischemia and further reperfusion, was investigated using the tensometric method. It was found that intravenous and intramuscular administration of С 60 fullerene with a single dose of 1 mg/kg exert different therapeutic effects dependent on the investigated macroparameters of muscle contraction. The intravenous drug administration was shown to be the most optimal for correction of the velocity macroparameters of contraction due to muscle tissue ischemic damage. In contrast, the intramuscular administration displays protective action with respect to motions associated with generation of maximal force response or continuous contractions elevating the level of muscle fatigue. Hence, С 60 fullerene, being a strong antioxidant, may be considered as a promising agent for effective therapy of pathological states of the muscle system caused by pathological action of free radical processes

Keywords: C[sub]60[/sub] fullerene, soleus muscle of rat, ischemia-reperfusion injury, dynamics of muscle contraction, dynamic light scattering.


  1. Murdock M, Murdoch MM. Compartment syndrome: a review of the literature. Clin Podiatr Med Surg. 2012; 29(2): 301-10. CrossRef PubMed
  3. Turóczi Z, Arányi P, Lukáts Á, Garbaisz D, Lotz G, Harsányi L, Szijártó A. Muscle fiber viability, a novel method for the fast detection of ischemic muscle injury in rats. PLoS One. 2014; 9(1): e84783.
  5. Rácz IB, Illyés G, Sarkadi L, Hamar J. The functional and morphological damage of ischemic reperfused skeletal muscle. Eur Surg. 1997; 29(4): 254-63. CrossRef  
  6. Loerakker S, Oomens CW, Manders E, Schakel T, Bader DL, Baaijens FP, Nicolay K, Strijkers GJ. Ischemiareperfusion injury in rat skeletal muscle assessed with T2-weighted and dynamic contrast-enhanced MRI. Magn Reson Med. 2011; 66(2): 528-37. CrossRef PubMed
  8. Cuzzocrea S, Riley DP, Caputi AP, Salvemini D. Antioxidant therapy: a new pharmacological approach in shock, inflammation, and ischemia/reperfusion injury. Pharmacol Rev. 2001; 53(1): 135-59.
  10. Prylutska S, Bilyy R, Overchuk M, Bychko A, Andreichenko K, Stoika R, Rybalchenko V, Prylutskyy Yu, Tsierkezos N, Ritter U. Water-soluble pristine fullerenes C60 increase the specific conductivity and capacity of lipid model membrane and form the channels in cellular plasma membrane. J Biomed Nanotechnol. 2012; 8: 522-7. CrossRef PubMed
  12. Gharbi N, Pressac M, Hadchouel M, Szwarc H, Wilson SR, Moussa F. C60 fullerene is a powerful antioxidant in vivo with no acute or subacute toxicity. Nano Lett. 2005; 5(12): 2578-85. CrossRef PubMed
  14. Prylutska SV, Grynyuk II, Matyshevska OP, Prylutskyy YuI, Ritter U, Scharff P. Anti-oxidant properties of C60 fullerenes in vitro. Fullerenes Nanotubes Carbon Nanostruct. 2008; 16: 698-705. CrossRef  
  15. Prylutska SV, Matyshevska OP, Golub Aa, Prylutskyy YuI, Potebnya GP, Ritter U, Scharff P. Study of S60 fullerenes and S60-containing composites cytotoxicity in vitro. Mater Sci Engineer. 2007; C 27: 1121-4.
  17. Prylutska SV, Burlaka AP, Prylutskyy YuI, Ritter U, Scharff P. Pristine C60 fullerenes inhibit the rate of tumor growth and metastasis. Exp Oncol. 2011; 33: 162-4.
  19. Prilutski Yu, Durov S, Bulavin L, Pogorelov V, Astashkin Yu, Yashchuk V, Ogul'chansky T, Buzaneva E, Andrievsky G. Study of structure of colloidal particles of fullerenes in water solution. Mol Cryst Liq Cryst. 1998; 324: 65-70. CrossRef  
  20. Mchedlov-Petrossyan NO. Fullerenes in liquid media: an unsettling intrusion into the solution chemistry. Chem Rev. 2013; 113(7): 5149-93. CrossRef PubMed
  22. Krustic PJ, Wasserman E, Keizer PN, Morton JR, Preston KF. Radical reactions of S60. Science. 1991; 254: 1183-5. CrossRef PubMed
  24. Prylutska SV, Burlaka AP, Matyshevska OP, Golub Aa, Potebnya GP, Prylutskyy YuI, Ritter U, Scharff P. Effect of the visible light irradiation of fullerene-containing composites on the ROS generation and the viability of tumor cells. Exp Oncol. 2006; 28(2): 160-2.
  26. Lai HS, Chen WJ, Chiang LY. Free radical scavenging activity of fullerenol on the ischemia-reperfusion intestine in dogs. World J Surg. 2000; 24(4): 450-4. CrossRef PubMed
  28. Huang SS, Tsai SK, Chih CL, Chiang LY, Hsieh HM, Teng CM, Tsai MC. Neuroprotective effect of hexasulfobutylated C60 on rats subjected to focal cerebral ischemia. Free Radic Biol Med. 2001; 30(6): 643-9. CrossRef  
  29. Scharff P, Carta-Abelmann L, Siegmund C, Matyshevska OP, Prylutska SV, Koval TV, Golub AA, Yashchuk VM, Kushnir KM, Prylutskyy YuI. Effect of X-ray and UV irradiation of the C60 fullerene aqueous solution on biological samples. Carbon. 2004; 42(5-6): 1199-201.
  31. Prylutska SV, Matyshevska OP, Grynyuk II, Prylutskyy YuI, Ritter U, Scharff P. Biological effects of C60 fullerenes in vitro and in a model system. Mol Cryst Liq Cryst. 2007; 468: 265-74. CrossRef  
  32. Nozdrenko DM, Bogutska KI, Prylutskyy YuI, Ritter U, Scharff P. C60 fullerene effect on the dynamics of fatigue processes in rat soleus muscle after ischemia-reperfusion. Biotechnol Acta. 2014; 7(3): 43-51. CrossRef  
  33. Ogloblya OV, Prylutskyy YuI, Strzhemechny YuM. Peculiarities of conductance of carbon nanotube-based quantum dots. Int J Quantum Chem. 2010; 110: 195-201. CrossRef  
  34. Rud Yu, Buchatskyy L, Prylutskyy Yu, Marchenko O, Senenko A, Schütze Ch, Ritter U. Using C60 fullerenes for photodynamic inactivation of mosquito iridescent viruses. J Enzyme Inhib Med Chem. 2012; 27(4): 614-7. CrossRef PubMed
  36. Wang WZ, Baynosa RC, Zamboni WA. Therapeutic interventions against reperfusion injury in skeletal muscle. J Surg Res. 2011; 171(1): 175-82. CrossRef PubMed
  38. Defraigne JO, Pincemail J. Local and systemic consequences of severe ischemia and reperfusion of the skeletal muscle. Physiopathol Rrevent Acta Chir Belg. 1998; 98(4): 176-86.
  40. Khoma OM, Zavodovskyi DA, Nozdrenko DM, Dolhopolov OV, Miroshnіchenko MS, Motuzjuk OP. Dynamics of ischemic skeletal soleus muscle contraction of the rat. Fiziol Zh. 2014; 60(1): 34-40 [Ukrainian].
  42. Carvalho AJ, McKee NH, Green HJ. Metabolic and contractile responses of fast and slow twitch rat skeletal muscles to ischemia and reperfusion. Plast Reconstr Surg. 1997; 99(1): 163-71. CrossRef PubMed
  44. Andreichenko KS, Prylutska SV, Medynska KO, Bogutska KI, Nurishchenko NE, Prylutskyy YuI, Ritter U, Scharff P. Effect of fullerene C60 on ATPase activity and superprecipitation of skeletal muscle actomyosin. Ukr Biochem J. 2013; 85(2): 20-6. CrossRef  
  45. Rácz IB, Sarkadi L, Hamar J. The functional damages of ischemic/reperfused skeletal muscle. Acta Physiol Hung. 1996; 84(3): 205-16.
  47. Korthals JK, Maki T, Gieron MA. Nerve and muscle vulnerability to ischemia. J Neurol Sci. 1985; 71(2-3): 283-90.

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