THE ENERGY FUNCTION OF RAT CARDIAC MITOCHONDRIA UNDER ARTIFICIAL HYPOBIOSIS
S.D. Melnytchuk1, S.V. Khyzhnyak1, V.S. Morozova1, L.I. Stepanova2, A.A. Umanskaya1, V.M. Voitsitsky1
- National University of Life and Environmental Sciences of
Ukraine
- Taras Shevchenko Kyiv National University
DOI: https://doi.org/10.15407/fz61.02.015
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Abstract
We investigated the energy activity of mitochondria from rat
cardiomyocytes under the artificial carbon dioxide hypobiosis,
which led to physiological changes in the organism (the
decrease of body temperature, the reduction of heart rate, etc.).
The respiratory and phosphorylation activities in mitochondria
of cardiomyocytes is reduced when using two oxidation
substrates (succinate and malate), which characterize the rate
of the oxygen consumption by the mitochondria. The partial
uncoupling of the oxidation and phosphorylation processes
when using the malate unlike succinate was established. The
activity of NADH-KoQ-oxidoreductase (complex I of the
respiratory chain) is inhibited, but the activities of succinate
dehydrogenase and cytochrome oxidase don’t change.
Probably, the priority of the succinate use under the artificial
hypobiosis provides the support of the mitochondria functional
activity on a sufficient energy level. It is evidenced by the
ATP-synthetase activity. The modifications of the structural
and functional state of the inner mitochondria membrane of
the cardiomyocytes are directed to the adaptation under the
artificial carbon dioxide hypobiosis
Keywords:
artificial hypobiosis, cardiomyocytes, mitochondria, respiratory chain, inner mitochondrial membrane, conformational modification.
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