Українська Русский English

ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2016; 62(5): 3-11


Experimental therapy of graft-versus-host disease by mesenchymal stromal cells grown on oxide nanocoatings

A.N. Goltsev1, I.V. Rassokha1, T.G. Dubrava1, L.V. Ostankova1, M.V. Ostankov1, V.I. Safonov2, A.V. Zykova2

  1. Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences, Kharkiv
  2. National Science Center ‘Kharkov Institute of Physics and Technology’ of the National Academy of Sciences, Kharkiv
DOI: https://doi.org/10.15407/fz62.05.003

Abstract

Immune aggression to transplanted allogeneic bone marrow, i.e. the graft-versus-host disease (GVHD), could be decreased by the suppression of effector and/or activation of T- regulatory cells (Тreg). This task could be solved by co-transplantaiton of allogeneic bone marrow and mesenchymal stromal cells (MSCs). This study demonstrated the elevated immune modulating activity of MSCs by their culturing in vitro on Al2O3 oxide nanocoatings. Introduction of the cells to the animals with GVHD resulted in an increased content of Treg in the spleen of bone marrow recipients, reduced severity of the pathology, and higher survival of animals. The findings could be the basis for developing the new approaches to optimize the GVHD treatment methods involving the oxide nanocoating cultured MSCs

Keywords: nanocoatings; mesenchymal stromal cells; graft-versus-host disease

References

  1. Shchepkina E A, Kruglyakov P V, Solomin L N, Zaritskiy Yu A, Nazarov V A, Viyde, et.al. Clinical experience with Experimental therapy of graft-versus-host disease by mesenchymal stromal cells grown on oxide nanocoatings transplantation of autologous mesenchymal stem cells in the treatment of false joints of long bones. Trauma. 2007; 8 (3):22-30. [Russian].
  2.  
  3. Sullivan M P, McHale K L, Parvizi J, Mehta S. Nanotechnology: current concepts in orthopaedic surgery and future directions. Bone Joint J 2014; 96 (5): 569-73. CrossRef PubMed
  4.  
  5. Webster T J. Nanomedicine: what's in a definition? Nanomedicine. Int J Nanomedicine. 2006; 1(2):169-76. CrossRef  
  6. Le Blanc K, Samuelsson H, Gustafsson B, Remberger M, Sundberg B, Arvidson J. et.al. Transplantation of mesenchymal stem cells to enhance engraftment of hematopoietic stem cells. Leukemia. 2006; 21(8):1733-38. CrossRef PubMed
  7.  
  8. Sergeev N S, Sviridov I K, Kirsanov V A, Akhmedova S A, Marshutina N V, Sergeev. In vitro study of parameters of the growth and differentiation of mesenchymal stromal cells of rat bone marrow in experiments in vitro. Cell Techn Biol Med. 2006;2: 102-7. [Russian].
  9.  
  10. Lee RH, Hsu SC, Munoz J, Jung JS, Lee NR. A subset of human rapidly-self renewing marrow stromal cells (MSCs) preferentially engraft in mice. Blood. 2006;107(5):2153-61. CrossRef PubMed
  11.  
  12. Goltsev AN, Rassokha IV, Dubrava TG, Ostankova LV, Ostankov MV, Gordienko EA. et.al. Application of nanocomposite coatings for multipotent mesenchymal stromal cells cultivation technologies. Cell Transplantol Tis Engin. 2013;8(1):46-50. [Ukrainian].
  13.  
  14. Le Blanc K, Frassoni F, Ball L, Locatelli F, Roelofs H, Lewis I, et.al. Mesenchymal stem cells for treatment of steroid-resistant, severe, acute graft-versus-host disease: a phase II study. Lancet. 2008;371(9624):1579-86. PubMed PMID: 18468541.
  15.  
  16. Fallarino F, Grohmann U, You S, McGrath B.C, Cavener DR, Vacca C. et.al. The combined effects of tryptophan starvation and tryptophan catabolites down-regulate T cell receptor z-chain and induce a regulatory phenotype in naive T cells. J Immunol. 2006;176(11):6752–61. CrossRef PubMed
  17.  
  18. Miura Y, Thoburn CJ, Bright EC, Phelps ML, Shin T, Matsui EC. et.al. Association of Foxp3 regulatory gene expression with graft-versus-host disease. Blood. 2004;104(7):2187-93. CrossRef PubMed
  19.  
  20. Sándor S,Tamás K, Katalin M, TiborT.G, Zoltán S, John V. Inhibition of indoleamine 2,3-dioxygenase-mediated tryptophan catabolism accelerates collagen-induced arthritis in mice. Arthr Res Ther. 2007 9:R50.
  21.  
  22. IDO-dependent immune modulating activity of cryopreserved fetal liver mesenchymal stem cells of mice in GVHR model Goltsev A., Dubrva T., Dimitrov A. Abstracts of the 4th Congress of the Ukrainian Society of Cell Biology with international representation (17-20 September 2014, Uzhgorod), с. 83. [Ukrainian].
  23.  
  24. Safonov V, Zykova A, Smolik J, Rogovska R, Donkov N, Goltsev A et.al. Nanoscale biomaterial interface modifications for advanced tissue engineering applications. J Phys. 2012; Conf. Ser. 356:012046 doi: 10.1088/1742- 6596/356/1/012046.
  25.  
  26. Shevelev, A.S. (1976) Graft versus host disease and transplantation disease. Moscow: "Meditsina". [Russian].
  27.  
  28. Donkov N, Zykova A, Safonov V, Smolik J, Rogowska R, Luk'yanchenko V. Formation of modern bearing surfaces by ceramic coating deposition for micro-bearing and biomedical applications. Rom Rep Phys. 2014;66(4):1180-8.
  29.  
  30. Sakaguchi S. Naturally arising Foxp3-expressing CD25+CD4+ regulatory T cells in immunologica tolerance to self and non-self. Nat Immunol. 2005;(6): 345-52.
  31.  
  32. Yarilin AA. Natural regulatory T-cells and the factor FOXP3. Immunologiya. 2006;(3):176-88.
  33.  
  34. Hirohata S. Role of bone marrow in pathogenesis rheumatoid arthritis. Cur Rheum Rev. 2006;(l2):47-54.
  35.  
  36. Goltsev AN, Lutsenko ED, Ostankova LV, Dubrava TG, Opanasenko EV. Application of cryobiology in solving immune-conflict problems when grafting a histocompatible bone marrow. Probl Cryobiol. 1996; (2):3-10. [Ukrainian].
  37. Goltsev AN. Experimental research as substantiation of the possibility of stem cell therapy in clinical practice. Col. of papers "Survival strategy in the context of bioethics, philosophy and medicine". Moldova, Kishinev: "Meditsina". 2014; 20(4):224-7. [Ukrainian].
  38.  
  39. Ku CH, Browne M, Gregson PJ, Pioletti DP. Large-scale gene expression analysis of osteoblasts cultured on three different Ti-6AL-4V surface treatments. Biomaterials. 2002;23(21):4193-202. CrossRef  
  40. Diener A, Nebe B, Uthen F, Becker P, Beck U, Neumann G. et.al. Control of focal adhesion dynamics by material surface characteristics. Biomaterials. 2005;26(4):383-92. CrossRef PubMed
  41.  
  42. Deligaianni DD, Katsala ND, Koutsoukos PG, Missirlis YF. Effect of surface roughness of hydroxyapatite on human bone marrow cell adhesion, proliferation, differentiation, and detachment strength. Biomaterials. 2001; 22 (1):87-96. CrossRef  
  43. Zykova A, Safonov V, Virva O, Luk'yanchenko V, Walkowich J, Rogowska R. et.al. Surface parameters modification by multilayer coatings deposition for biomedical applications. J Phys Conf Ser. 2008; IOP Publishing. (113):15-9.
  44.  
  45. Kato S, Akagi T, Sugimura K, Kishida A, Akashi M. Evalution of biological responses to polymeric by RTPCR analysis IV: study of cmyc, c-fos and p53 mRNA expression. Biomaterials. 2000;(21):521 –7.
  46.  
  47. Goltsev AN, Rassokha IV, Dubrava TG, Ostankova LV, Ostankov MV, Gordienko. et.al. Application of nanocomposite coatings with different structural physical and chemical characteristics in tissue engineering. J Nan Electr Phys. 2013;5(1): 01008 (5pp).
  48.  
  49. Sugita S, Futagami Y, Hori S, Mochizuki M. Transforming growth factor producing FoxP3+CD4+CD25+ T cells by iris pigment epithelial cells display regulatory phenotype and acquire regulatory functions. Exp res. 2007;85(5):626-36.
  50.  
  51. Goltsev AN, Dubrava TG, Lutsenko ED, Ostankova LV, Matsevitaya IYu, Ostankov V. et.al. Manifestation of immune correcting effect of cryopreserved cells of fetal liver of different gestation terms under development conditions of experimental model of graft versus host reaction. Cel transplant tis engineer. 2010;5(3):82-6. [Ukrainian].
  52.  

© National Academy of Sciences of Ukraine, Bogomoletz Institute of Physiology, 2014-2019.