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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2015; 61(6): 3-10

HYDROGEN SULFIDE DONOR, NAHS, RECOVERS CONSTITUTIVE NO SYNTHESIS AND ENDOTHELIUM-DEPENDENT RELAXATION OF ISOLATED AORTA IN OLD RATS

K.O. Drachuk, A.V. Kotsjuruba, V.F. Sagach

    Bogomoletz Institute of Physiology, Kyiv, Ukraine
DOI: https://doi.org/10.15407/fz61.06.003


Abstract

The objective of this study was to show the effect of H2S donor, NaHS on the endothelium-dependent vasorelaxation, free radical state and cNOS uncoupling in old rats. In the aorta of old rats a combined oxidative and nitrosative stress develops that leads to сNOS uncoupling and decreased constitutive synthesis of the NO. That biochemical changes correlate with lowering of the endothelium-dependent relaxation of aortic smooth muscles (7,5 ± 1,4,%, compared with 64,9 ± 3,5,% in adults) . It was found that, due to the combined inhibition of oxidative and nitrosative stress, NaHS restores constitutive de novo synthesis of NO by restoring сNOS coupling. Additionally, NaHS improves endothelium-dependent vasorelaxation by increasing (by 6,5 times) Ach-induced relaxation of aortic smooth muscles

Keywords: aging; endothelial dysfunction; oxidative stress; nitrosative stress; cNOS uncoupling; hydrogen sulfide; nitric oxide

Full text: (PDF, in Ukrainian)

References

  1. Francis SH, Busch JL, Corbin JD, Sibley D. cGMPdependentprotein kinases and cGMP phosphodiesterasesin nitric oxide and cGMP action. Pharmacol Rev.2010;62(3):525-63. CrossRef PubMed PubMedCentral
    2.  
  2. Van Hove CE, Van der Donckt C, Herman AG, Bult H,Fransen P. Vasodilator efficacy of nitric oxide dependson mechanisms of intracellular calcium mobilization inmouse aortic smooth muscle cells. Br J Pharmacol. 2009;158(3):920-30. CrossRef PubMed PubMedCentral
    3.  
  3. Soloviev A, Lehen'kyi V, Zelensky S, Hellstrand P. Nitricoxide relaxes rat tail artery smooth muscle by cyclicGMP-independent decrease in calcium sensitivity ofmyofilaments. Cell Calcium. 2004;36(2):165-73. CrossRef PubMed
    4.  
  4. El Assar M, Angulo J, Vallejo S, Peiró C, Sánchez-FerrerCF, Rodríguez-Ma-as L. Mechanisms involved in theaging-induced vascular dysfunction. Front Physiol.2012;28;3:132.
    5.  
  5. Kondo K, Bhushan S, King AL, Prabhu SD, Hamid T,Koenig S, et al. H2S protects against pressure overloadinducedheart failure via upregulation of endothelialnitric oxide synthase. Circulation. 2013;127:1116–1127. CrossRef PubMed PubMedCentral
    6.  
  6. Streeter E, Ng HH, Hart JL. Hydrogen sulfide as a vasculoprotectivefactor. Med Gas Res. 2013;3(1):9. CrossRef PubMed PubMedCentral
    7.  
  7. Qiao WL, Yang WX, Liu L, Shi Y, Cui J, Liu H, et al.Exogenous hydrogen sulfide reduces vascular aging inD-galactose-induced subacute aging rats. Sheng Li XueBao. 2014;66(3):276-82.
    8.  
  8. King AL, Polhemus DJ, Bhushan S, Otsuka H, Kondo K,Nicholson CK, et al. Hydrogen sulfide cytoprotective signalingis endothelial nitric oxide synthase-nitric oxide dependent.Proc Natl Acad Sci. U S A. 2014;111(8):3182-7. CrossRef PubMed PubMedCentral
    9.  
  9. Bucci M, Papapetropoulos A, Vellecco V, Zhou Z, PyriochouA, Roussos C, et al. Hydrogen sulfide is an endogenousinhibitor of phosphodiesterase activity. ArteriosclerThromb Vasc Biol. 2010;30(10):1998-2004. CrossRef PubMed
    10.  
  10. Coletta C, Papapetropoulos A, Erdelyi K, Olah G, MódisK, Panopoulos P, et al. Hydrogen sulfide and nitric oxideare mutually dependent in the regulation of angiogenesisand endothelium-dependent vasorelaxation. Proc NatlAcad Sci U S A. 2012;109(23):9161-6. CrossRef PubMed PubMedCentral
    11.  
  11. Bełtowski J, Jamroz-Wiśniewska A. Hydrogen sulfide andendothelium-dependent vasorelaxation. Molecules. 2014;19(12):21183-99. CrossRef PubMed
    12.  
  12. Drachuk KO, Kotsjuruba AV, Bazilyuk OV, StepanenkoLG, Sagach VF. Propargylglycine restores endotheliumdependentrelaxation of aortic smooth muscle in old rats.Fiziol Zh. 2014; 60(4):3-10. [Ukrainian].
    13.  
  13. Sharipov RR, Kotsiuruba AV, Kop'iak BS, Sahach VF.Induction of oxidative stress in heart mitochondriain brain focal ischemia-reperfusion and protectiveeffect of ecdysterone. Fiziol Zh. 2014; 60(3):11-7.[Ukrainian].
    14.  
  14. Dorofeyeva NA, Kotsuruba AV, Kopjak BS, Sagach VF.Physical exercise training cancels sonstitutive nos uncouplingand induced violations of cardiac hemodynamicsin hypertension (part III). Fiziol Zh. 2015; 61(4):11-21[Ukrainian].
    15.  
  15. Sharipov RR, Kotsiuruba AV, Kop'iak BS, Sahach VF. Inductionof nitrosative stress in mitochondria of rats heartsin experimental ischemia-reperfusion of the brain and itscorrection by ecdysterone. Fiziol Zh. 2014; 60(5):3-13.[Ukrainian].
    16.  
  16. Kotsiuruba AV, Dorofeyeva NA, Sagach VF. NOSuncoupling is accompanied with induction of the oxidativestress and the cardiohemodynamics disturbances inhypertension. Fiziol Zh. 2015; 61(3):3-10. [Ukrainian].
    17.  
  17. Sergent O, Morel I, Cogrel P, Chevanne M, Pasdeloup N,Brissot P, et al. Simultaneous measurements of conjugateddienes and free malondialdehyde, used as a micromethodfor the evaluation of lipid peroxidation in rat hepatocytecultures. Chem Phys Lipids. 1993; 65(2):133-9. CrossRef  
  18. Beckman JS, Koppenol WH. Nitric oxide, superoxide, andperoxynitrite: the good, the bad, and ugly. Am J Physiol.1996; 271(5):1424-37.
    19.  
  19. Szabó C. The pathophysiological role of peroxynitrite inshock, inflammation, and ischemia-reperfusion injury.Shock. 1996; 6(2):79-88. CrossRef PubMed
    20.  
  20. Pryor WA, Squadrito GL. The chemistry of peroxynitrite: aproduct from the reaction of nitric oxide with superoxide.Am J Physiol. 1995;268(5):699-722.
    21.  

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