HYDROGEN SULFIDE DONOR, NAHS, RECOVERS CONSTITUTIVE NO SYNTHESIS AND ENDOTHELIUM-DEPENDENT RELAXATION OF ISOLATED AORTA IN OLD RATS
K.O. Drachuk, A.V. Kotsjuruba, V.F. Sagach
Bogomoletz Institute of Physiology, Kyiv, Ukraine
DOI: https://doi.org/10.15407/fz61.06.003
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Abstract
The objective of this study was to show the effect of H2S donor,
NaHS on the endothelium-dependent vasorelaxation, free radical state and cNOS uncoupling in old rats. In the aorta of
old rats a combined oxidative and nitrosative stress develops
that leads to сNOS uncoupling and decreased constitutive
synthesis of the NO. That biochemical changes correlate with
lowering of the endothelium-dependent relaxation of aortic
smooth muscles (7,5 ± 1,4,%, compared with 64,9 ± 3,5,% in
adults) . It was found that, due to the combined inhibition of
oxidative and nitrosative stress, NaHS restores constitutive de
novo synthesis of NO by restoring сNOS coupling. Additionally,
NaHS improves endothelium-dependent vasorelaxation
by increasing (by 6,5 times) Ach-induced relaxation of aortic
smooth muscles
Keywords:
aging; endothelial dysfunction; oxidative stress; nitrosative stress; cNOS uncoupling; hydrogen sulfide; nitric oxide
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