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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2015; 61(1): 19-27

Association of allelic polymorphisms of genes matrix Gla-protein system with ischemic atherothrombotic stroke

V.Yu. Garbuzova1, D.A. Stroy2, V.E. Dosenko2, Ye.I. Dubovyk1, A.O. Borodenko1, K.A. Shimko1, O.A. Obukhova1, O.V. Ataman1

  1. Sumy State University
  2. O.O.Bogomoletz Institute of Physiology of NAS Ukraine, Kyiv
DOI: https://doi.org/10.15407/fz61.01.019


Abstract

There are results of the determination of 10 polymorphisms of matrix Gla-protein system (gene MGP – T-138→С (rs1800802), G-7→A (rs1800801), Thr83→Ala (rs4236), gene VDR – FokI (rs2228570), BsmI (rs1544410), ApaI (rs7975232 ), TaqI (rs731236), gene GGCX – Arg325→Gln (rs699664), gene VKORS1 – T2255→C (rs2359612), gene BMP-2 – Ser37→Ala (rs2273073)) into 170 patients with ischemic atherothrombotic stroke (IATS) and 124 healthy individual is (control group). It is established that there is a connection between the IATS and polymorphic variants of genes MGP (G-7→A) and VKORC1 (T2255→C). The risk of IATS in carriers of minor allele A/A (G-7→A polymorphism) in 2.6 times higher than in carriers of the major allele (G/A + G/G), and C/C genotype (T2255→C polymorphism) in 2.2 times higher than the homozygotes of major allele. The coincidence of patients T/C and G/G, C/C and G/A genotypes, and A/A genotype (G-7→A polymorphism) with any genotype T2255→C polymorphism are increases the risk of IATS.

Keywords: matrix Gla-protein; gene polymorphism; ischemic atherothrombotic stroke

Full text: (PDF, in Ukrainian)

References

  1. Abedin M, Tintut Y, Demer L. Vascular calcification.Mechanisms and clinical ramifications. ArteriosclerThromb Vasc Biol. 2004; 24:1161-70. CrossRef PubMed
    2.  
  2. Johnson RC, Leopold JA, Loscalzo J. Vascular calcification.Pathobiological mechanisms and clinical implications.Circ Res. 2006; 99:1044-59. CrossRef PubMed
    3.  
  3. Lanzer P. Mediakazinose Mönckeberg. Z Kardiol. 1998;87:586-93. CrossRef PubMed
    4.  
  4. Lehto S, Niskanen L, Suhonen M. Medial artery calcification.A neglected harbinger of cardiovascular complicationsin non-insulin-dependent diabetes mellitus.Arterioscler Thromb Vasc Biol. 1996; 16(8):978-83. CrossRef PubMed
    5.  
  5. Garbuzova VYu, Ataman AV. Matrix Gla-Protein and ItsRole in Vascular Calcification. Int J Physiol Pathophysiol.2012; 3(1):79-99. CrossRef  
  6. Proudfoot D, Shanahan CM. Molecular mechanismsmediating vascular calcification: role of matrix Gla protein.Nephrology (Carlton). 2006; 11:455-61. CrossRef PubMed
    7.  
  7. Ataman AV, Garbuzova VYu, Ataman YuA, Matlaj OI,Obukhova OA. Investigation of the MGP promoter andexon 4 polymorphisms in patients with ischemic stroke inthe Ukrainian population. Journal of Cell and MolecularBiology. 2012; 10(1):19-26.
    8.  
  8. Crosier MD, Booth SL, Peter I, Dawson-Hughes B, Price PA,O'Donnell CJ, et al. Matrix Gla protein polymorphismsare associated with coronary artery calcification. J NutrSci Vitaminol. 2009; 55:59-65. CrossRef  
  9. Garbuzova VYu, Gurianova VL, Stroy DA, Dosenko VE,Parkhomenko AN, Ataman AV. Association of matrix Glaprotein gene allelic polymorphisms (G7>A, T-138>C andThr83>Ala) with acute coronary syndrome in the Ukrainianpopulation. Exp Clin Cardiol. 2012; 17(1):30-33.
    10.  
  10. Herrmann SM, Whatling C, Brand E, Nikaud V, GariepyJ, Simon A, et al. Polymorphisms of the human matrixGla protein (MGP) gene, vascular calcification, andmyocardial infarction. Arterioscler Thromb Vasc Biol.2000; 20:2386-93. CrossRef PubMed
    11.  
  11. Kobayashi N, Kitazawa R, Maeda S, Schurgers LJ,Kitazawa S. T-138C polymorphism of matrix Gla proteinpromoter alters its expression but is not directly associatedwith atherosclerotic vascular calcification. Kobe J MedSci. 2004; 50:69-81.
    12.  
  12. Kim JG, Ku SY, Lee DO. Relationship of osteocalcinand matrix Gla protein gene polymorphisms to serumosteocalcin levels and bone mineral density inpostmenopausal Korean women. Menopause. 2006;13:467-73. CrossRef PubMed
    13.  
  13. Gao B, Yasui T, Itoh Y, Tozawa K, Hayashi Y, Kohri K.Apolymorphism of matrix Gla protein gene is associatedwith kidney stones. J Urol. 2007; 177:2361-65. CrossRef PubMed
    14.  
  14. Hirano H, Ezura Y, Ishiyama N, Yamaguchi M, Nasu I,Yoshida H, et al. Association of natural tooth loss withgenetic variation at the human matrix Gla protein locus inelderly women. J Hum Genet. 2003; 48:288-92.
    15.  
  15. Shaik AP, Kaiser J. Polymorphisms in MGP gene and theirassociation with lead toxicity. Toxicol Mech Methods.2009; 19:209-13. CrossRef PubMed PubMedCentral
    16.  
  16. Adams HP, Bendixen BH, Kappelle LJ, Biller J, LoveBB, Gordon DL, et al. Classification of subtype of acuteischemic stroke. Definitions for use in a multicenterclinical trial. TOAST. Trial of Org 10172 in Acute StrokeTreatment. Stroke. 1993; 24:35-41. CrossRef PubMed
    17.  
  17. Breiman L. Random Forests. Machine Learning. 2001;45:5-32. CrossRef  
  18. Breiman L, Friedman JH, Olshen RA. Classification andRegression Trees. 1st ed. Belmont: Wadsworth; 1984.
    19.  
  19. Bureau A, Dupuis J, Falls K, Lunetta KL, Hayward B,Keith TP, Eerdewegh PV. Identifying SNPs Predictive ofPhenotype Using Random Forests. Genetic Epidemiology.2005; 28:171-82. CrossRef PubMed
    20.  
  20. Strobl C, Voulesteix AL, Kneib T, Augustin T, Zeileis A.Conditional variable importance for random forests. BMCBioinformatics. 2008; 9:307. CrossRef  
  21. Alison AM, Marylyn D. Multifactor dimensionalityreduction: An analysis strategy for modelling anddetecting gene – gene interactions in human geneticsand pharmacogenomics studies. Numan genomics. 2006;2(5):318-28.
    22.  
  22. Shyu HY, Fong CS, Fu YP, Shieh JC, Yin JH, Chang CY,et al. Genotype polymorphisms of GGCX, NQO1, andVKORC1 genes associated with risk susceptibility in patientswith large-artery atherosclerotic stroke. Clin ChimActa. 2010; 411:840-5. CrossRef PubMed
    23.  
  23. Porojan M, Dumitraşcu DL. Genetic polymorphismof VKORC 1 and KLOTHO genes associated withatherosclerosis. Clujul Medical. 2012; 85(4):533-6.
    24.  
  24. Wang Y, Zhang W, Zhang Y, Yang Y, Sun L, Hu S, etal. VKORC1 Haplotypes Are Associated With ArterialVascular Diseases (Stroke, Coronary Heart Disease, andAortic Dissection. Circulation. 2006; 113:1615-21. CrossRef PubMed
    25.  
  25. Hindorff LA, Heckbert SR, Smith N, Marciante KD, PsatyBM. Common VKORC1 variants are not associated witharterial or venous thrombosis. Journal of Thrombosis andHaemostasis. 2007; 5:2025-27. CrossRef PubMed
    26.  

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