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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2014; 60(6): 46-53


PROPERTIES OF ERYTHROCYTES WITH LOW DEGREE OF INTEGRITY AFTER FREEZING WITH NON-PENETRATING AND PENETRATING CRYOPROTECTANTS

V.V. Ramazanov, E.L. Volovelskaya, V.A. Koptelov, V.A. Bondarenko

    Institute for Problems of Cryobiology and CryomedicineNational Academy of Sciences of Ukraine, Kharkov, Ukraine
DOI: https://doi.org/10.15407/fz60.06.046

Abstract

Osmotic, antioxidant and morphological characteristics of erythrocytes frozen in liquid nitrogen (-196°C) in the medium containing sucrose, dextran and DMSO were studied. During freezing in the medium containing sucrose and sucrose and dextran there is noted the disorder in osmotic and morphological characteristics of the rest of cells after washing-out from cryopreservative. We have not revealed a significant change in concentration of malondialdehyde and the indices of activity of glutathione-dependent enzymes (glutathione reductase and glutathione peroxidase). Inclusion of DMSO into the mentioned media results in the decreased degree of erythrocytes damage during freezing and preservation of osmotic, antioxidant and morphological indices of the rest of cells independent of the degree of their integrity after washing-out from cryopreservative. The obtained results indicate that in order to preserve the properties of erythrocytes during freezing the cryopreservative should include the combination of non-penetrating and penetrating cryoprotectants.

Keywords: erythrocytes, osmotic, antioxidant and morphologicalproperties, freezing, combined cryopreservative.

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