PROPERTIES OF ERYTHROCYTES WITH LOW DEGREE OF INTEGRITY AFTER FREEZING WITH NON-PENETRATING AND PENETRATING CRYOPROTECTANTS
V.V. Ramazanov, E.L. Volovelskaya, V.A. Koptelov, V.A. Bondarenko
Institute for Problems of Cryobiology and CryomedicineNational Academy of Sciences of Ukraine, Kharkov, Ukraine
Osmotic, antioxidant and morphological characteristics of
erythrocytes frozen in liquid nitrogen (-196°C) in the medium
containing sucrose, dextran and DMSO were studied. During
freezing in the medium containing sucrose and sucrose and
dextran there is noted the disorder in osmotic and morphological
characteristics of the rest of cells after washing-out from
cryopreservative. We have not revealed a significant change
in concentration of malondialdehyde and the indices of activity
of glutathione-dependent enzymes (glutathione reductase
and glutathione peroxidase). Inclusion of DMSO into the
mentioned media results in the decreased degree of erythrocytes
damage during freezing and preservation of osmotic,
antioxidant and morphological indices of the rest of cells
independent of the degree of their integrity after washing-out
from cryopreservative. The obtained results indicate that in
order to preserve the properties of erythrocytes during freezing
the cryopreservative should include the combination of
non-penetrating and penetrating cryoprotectants.
erythrocytes, osmotic, antioxidant and morphologicalproperties, freezing, combined cryopreservative.
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