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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2014; 60(6): 10-15

THE IMPACT OF PHARMACOLOGICAL ACTIVATION OF ATP-SENSITIVE POTASSIUM CHANNELS ON THE PARAMETERS OF SPONTANEOUS ACTIVITY OF ISOLATED NEONATAL RAT CARDIOMYOCYTES

K.V. Tarasova1, T.S. Lagodych1, T.I. Drevytska2, S.B. Frantsuzova2, I.M. Karvatsky1

  1. Bogomoletz National University, Kyiv, Ukraine
  2. O.O.Bogomoletz Institute of Physiology of NAS Ukraine, Kyiv, Ukraine
DOI: https://doi.org/10.15407/fz60.06.010


Abstract

We investigated spontaneous contractile activity of isolated cultured neonatal cardiomyocytes. It was shown that contractile activity of isolated cardiomyocytes depends on the duration of culture. Culture from 48 to 72 or 96 hours the amplitude of contraction grows (from 1,96±0,362 to 2,55±0,28 micrometers), frequency (from 59,4±6,59 to 84±5,92 contractions by minute) contraction speed (from10,39±1,92 to 16,36±2,17 micrometers per second) and relaxation (from 7,16±1,89 to 10,95±0,82 micrometers per second). Comparative study of the effect of diazoxide and its fluorine analog has shown that the activators of ATP-sensitive potassium channels influence the contractile function of isolated cardiomyocytes in different degrees: diazoxide causes its full termination, but its fluorine analog evokes only minor changes: a peak-reaching time decreases (from 0,16±0,02 to 0,15±0,15 seconds), contraction amplitude decreases (from1,91±0,39 to 1,47±0,28 micrometers), relaxation speed decreases (from 9,53±1,62 to 7,82±1,1 micrometers per second) with constant frequency and contraction speed. A minor inhibitory effect of fluorine analog of diazoxide on the contractile activity of neonatal cardiomyocytes is an advantage of this agent compared with its prototype diazoxide. Key words: isolated neonatal cardiomyocytes, the parameters of the spontaneous contractile activity, activators of the КATP channels.

Keywords: isolated neonatal cardiomyocytes, the parametersof the spontaneous contractile activity, activators of the КATPchannels.

Full text: (PDF, in Ukrainian)

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