THE IMPACT OF PHARMACOLOGICAL ACTIVATION OF ATP-SENSITIVE POTASSIUM CHANNELS ON THE PARAMETERS OF SPONTANEOUS ACTIVITY OF ISOLATED NEONATAL RAT CARDIOMYOCYTES
K.V. Tarasova1, T.S. Lagodych1, T.I. Drevytska2, S.B. Frantsuzova2, I.M. Karvatsky1
- Bogomoletz National University, Kyiv, Ukraine
- O.O.Bogomoletz Institute of Physiology of NAS Ukraine, Kyiv, Ukraine
We investigated spontaneous contractile activity of isolated
cultured neonatal cardiomyocytes. It was shown that contractile
activity of isolated cardiomyocytes depends on the duration
of culture. Culture from 48 to 72 or 96 hours the amplitude
of contraction grows (from 1,96±0,362 to 2,55±0,28 micrometers),
frequency (from 59,4±6,59 to 84±5,92 contractions by
minute) contraction speed (from10,39±1,92 to 16,36±2,17
micrometers per second) and relaxation (from 7,16±1,89 to
10,95±0,82 micrometers per second). Comparative study of
the effect of diazoxide and its fluorine analog has shown that
the activators of ATP-sensitive potassium channels influence
the contractile function of isolated cardiomyocytes in different
degrees: diazoxide causes its full termination, but its
fluorine analog evokes only minor changes: a peak-reaching
time decreases (from 0,16±0,02 to 0,15±0,15 seconds), contraction
amplitude decreases (from1,91±0,39 to 1,47±0,28
micrometers), relaxation speed decreases (from 9,53±1,62 to
7,82±1,1 micrometers per second) with constant frequency
and contraction speed. A minor inhibitory effect of fluorine
analog of diazoxide on the contractile activity of neonatal
cardiomyocytes is an advantage of this agent compared with
its prototype diazoxide.
Key words: isolated neonatal cardiomyocytes, the parameters
of the spontaneous contractile activity, activators of the КATP
isolated neonatal cardiomyocytes, the parametersof the spontaneous contractile activity, activators of the КATPchannels.
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