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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2014; 60(6): 10-15


THE IMPACT OF PHARMACOLOGICAL ACTIVATION OF ATP-SENSITIVE POTASSIUM CHANNELS ON THE PARAMETERS OF SPONTANEOUS ACTIVITY OF ISOLATED NEONATAL RAT CARDIOMYOCYTES

K.V. Tarasova1, T.S. Lagodych1, T.I. Drevytska2, S.B. Frantsuzova2, I.M. Karvatsky1

  1. Bogomoletz National University, Kyiv, Ukraine
  2. O.O.Bogomoletz Institute of Physiology of NAS Ukraine, Kyiv, Ukraine
DOI: https://doi.org/10.15407/fz60.06.010

Abstract

We investigated spontaneous contractile activity of isolated cultured neonatal cardiomyocytes. It was shown that contractile activity of isolated cardiomyocytes depends on the duration of culture. Culture from 48 to 72 or 96 hours the amplitude of contraction grows (from 1,96±0,362 to 2,55±0,28 micrometers), frequency (from 59,4±6,59 to 84±5,92 contractions by minute) contraction speed (from10,39±1,92 to 16,36±2,17 micrometers per second) and relaxation (from 7,16±1,89 to 10,95±0,82 micrometers per second). Comparative study of the effect of diazoxide and its fluorine analog has shown that the activators of ATP-sensitive potassium channels influence the contractile function of isolated cardiomyocytes in different degrees: diazoxide causes its full termination, but its fluorine analog evokes only minor changes: a peak-reaching time decreases (from 0,16±0,02 to 0,15±0,15 seconds), contraction amplitude decreases (from1,91±0,39 to 1,47±0,28 micrometers), relaxation speed decreases (from 9,53±1,62 to 7,82±1,1 micrometers per second) with constant frequency and contraction speed. A minor inhibitory effect of fluorine analog of diazoxide on the contractile activity of neonatal cardiomyocytes is an advantage of this agent compared with its prototype diazoxide. Key words: isolated neonatal cardiomyocytes, the parameters of the spontaneous contractile activity, activators of the КATP channels.

Keywords: isolated neonatal cardiomyocytes, the parametersof the spontaneous contractile activity, activators of the КATPchannels.

References

  1. Belostotskaya GB, Darashina IV, Golovanova TA, Khrustaleva RS, Tsyrlin VA. Assessment of the functional state of freshly isolated and cultured cardiomyocytes of rats nder conditions of oxidative stress. Regional haemodyn. microcircul. 2008;7(2):85-92.
  2.  
  3. Xia Y, Rajapurohitam V, Cook MA, Karmazyn M. Inhibition of phenylephrine induced hypertrophy in rat neonatal cardiomyocytes by the mitochondrial KATP channel opener diazoxide. J Mol Cell Cardiol. 2004; 37(5):1063-67. CrossRef PubMed
  4.  
  5. Maffit SK, Sellitto AD, Al-Dadah AS, Schuessler RB, Damiano RJ, Lawton JS. Diazoxide maintains human myocyte volume homeostasis during stress. Jam Heart Assoc. 2012; 1(2):1-8. CrossRef  
  6. Al-Dadah AS, Voeller R K, Schuessler RB, Damiano RJ, Lawton JS. Maintenance of myocyte volume homeostasis during stress by diazoxide is cardioprotective. Ann Thorac Surg. 2007; 84(3):857-62. CrossRef PubMed
  7.  
  8. Tarasova KV, Shevchuk VG, Grygoruk OV, Frantsuzova SB, Lisova GO. Research of structural changes of a myocardium of rats under long influence of potassium channels activators (diazoxide and it fluorine containing analogues). JVinn Med Univers. 2007; 11(2/1):538-41.
  9.  
  10. KV Tarasova, LL Arshinnikova, LI Antonenko, SB Frantsuzova, VG Shevchuk. Effect of long administration of activators of KATP channels on the energy supplying of myocardium and the permeability of cell membranes. Pharmacology and Drug Toxicology. 2011;4:51-56.
  11.  
  12. Tarasova EV, Shevchuk VG Influence of KATR activators (diazoxid and its fluorinated analogues) on contractile activity of isolated papillary muscle of heart of old rats. Ukrainian Medical Almanac.2009; 12 (2):245-48.
  13.  
  14. Kicinska A, Szewczyk A. Protective effects of the potassium channel opener-diazoxide against injury in neonatal rat ventricular myocytes.Gen Physiol Biophys.2003; 22:383-395. PubMed
  15.  
  16. Reinecke H, Zhang M, Bartosek T, Murry CE. Survival, integration, and differentiation of cardiomyocyte graftsa study in normal and injured rat hearts.Circulation. 1999;100:193-202. CrossRef PubMed
  17.  
  18. Dosenko VE, Nagibin VS, Tumanovskaya LV, Moibenko AA, Vaage J. Postconditioning prevents apoptotic, necrotic and autophagic cardiomyocyte cell death in culture. Fiziol Zh.2005; 51:12-17. PubMed
  19.  
  20. Webster DR, Patrick DL. Beating rate of isolated neonatal cardiomyocytes is regulated by the stable microtubule subset.AJP Heart. 2000; 278 (5):H1653-H1661. CrossRef  
  21. Lalevee N, Rebsamen MC, Barre`re-Lemaire S, Perriera E, Nargeot J, Benitah J-P, Rossier MF. Aldosterone increases T-type calcium channel expression and in vitro beating frequency in neonatal rat cardiomyocytes. Cardiovasc. Res.2005; 67:216-224. CrossRef PubMed
  22.  
  23. Lancaster JJ, Johnson NM, Juneman E, Thai HM, Bahl J, Goldman S. Construction of a spontaneously contracting biologically active cardiomyocyte scaffold. J Cardiac failure. 2009; 15(6):4-5. CrossRef  
  24. Er F, Larbig R, Ludwig A, Biel M, Hofmann F, Beuckelmann DJ, Hoppe UC. Dominant-negative suppression of HCN channels markedly reduces the native pacemaker current If and undermines spontaneous beating of neonatal sardiomyocytes. Circulation. 2003; 107:485-89. CrossRef PubMed
  25.  
  26. Lorenzen-Schmidt I, Schmid-Schonbein GW, Giles WR, McCulloch AD, Chien S, Omens JH. Chronotropic response of cultured neonatal rat ventricular myocytes to short-term fluid shear.Cell. Biochem. Biophys. 2006; 46 (2):113-22. CrossRef  
  27. Jung AS, Kubo H, Wilson R, Houser SR, Margulies KB.Modulation of contractility by myocyte-derived arginase in normal and hypertrophied feline myocardium. Am J Physiol. 2006; 290 (5):H1756-H1762. CrossRef  
  28. Kocic I, Gruchala M, Petrusewicz J. Gender differences in effects of pinacidil but not diazoxide on heart automatism in the isolated guinea pig right atria. Pol J Pharmacol. 2003; 55(3):419-24. PubMed
  29.  
  30. De Giusti VC, Correa MV, Beltrano S, Yeves AM, Villa- Abrille MS, Chiappe de Cingolani GE, Cingolani NE, Aiello AE. The positive inotropic effect of endothelin-1 is mediated by mitochondrial reactive oxygen species.Life Sciences.2008; 83:264-71. CrossRef PubMed
  31.  
  32. Deja MA, Golba KS, Kolowca M, Widenka K, Biernat J, Wos S. Diazoxide provides protection to human myocardium in vitro that is concentration dependent. Ann Thorac Surg.2004; 77: 226-32. CrossRef  

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