Українська English

ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2014; 60(5): 89-98

Dynamics of structural change of ovarian tissue under ischemia

Bozhkova IuO, Kiroshka VV, Havas AA, Bondarenko VA

    Institute for Problem of Cryobiology and Criomedicine ofNAS of Ukraine, Kharkiv, Ukraine


In this work it was investigated the dependence of the correlation of folliculogenesis and endocrine function of ovarian tissue on the degree of structural damage and oocyte volume changes during ischemia. It was shown that after 2 hours of ischemia at 37 °C the morphological transformation of the structural components of the ovarian tissue were reversible. In case of restoration of blood flow conditions by heterotopic transplantation estradiol level of animals was 25,9 ± 5,18 pg/ ml, progesterone - 18,48 ± 3,69 ng/ml, significantly higher than the castrated animals-recipients. Supplement of the incubation medium by the fetal bovine serum lead to decreasing by 5-7 % in the volume of oocytes of growing follicles during in ischemia and reduced steroidogenic function of ovarian tissue after perfusion. Increased time of the ischemia up to 4 hours was founded in irreversible morphological transformation, reduce the volume of oocytes by 40 % and the formation of sclerosed tissue after transplantation of the ischemic fragments of ovarian tissue.

Keywords: ovarian tissue, ischemic damage, endocrine function,the volume of oocyte.


  1. Grace PA. Ischemia-reperfusion injury. Brit J Surg. 1994;81:631-41. CrossRef  
  2. Dorweiler B, Pruefer D, Andrasi TB, Maksan SM, Schmiedt W, Neufang A, et al. Ischemia-Reperfusion Injury. Eur J Trauma Emerg Surg . 2007;33(6):600-12. CrossRef PubMed
  4. Kerrigan CL, Stotland MA. Ischemia reperfusion injury: a review. Microsurgery. 1993;14:165-75. CrossRef  
  5. Piper HM, Meuter K, Schafer C. Cellular mechanisms of ischemia-reperfusion injury. Ann Thorac Surg. 2003;75:644-8. CrossRef  
  6. Fu H, Xu H, Chen H, Li Y, Li W, Zhu Q, et al. Inhibition of Glycogen Synthase Kinase 3 Ameliorates Liver Ischemia/Reperfusion Injury via an Energy-dependent Mitochondrial Mechanism. J Hepatol. 2014 May;23:358- 4. CrossRef  
  7. Ozturk H, Ozturk H, Terzi EH, Ozgen U, Duran A, Uygun I. Protective effects of rosmarinic acid against renal ischaemia/reperfusion injury in rats. J Pak Med Assoc. 2014 Mar;64(3):260-5. PubMed
  9. Alvarez P, Tapia L, Mardones LA, Pedemonte JC, Farias JG, Castillo RL. Cellular mechanisms against ischemia reperfusion injury induced by the use of anesthetic pharmacological agents. Chem Biol Interact. 2014 May 13;218:89-98. CrossRef PubMed
  11. Reilly PM, Schiller HJ, Bulkley GB. Pharmacologic approach to tissue injury mediated by free radicals and other reactive oxygen metabolites. Amer J Surg. 1991;161:488-504. CrossRef  
  12. Mallick IH, Yang W, Winslet MC, Seifalian AM. Ischemia– reperfusion injury of the intestine and protective strategies against injury. Digestive Diseases and Sciences. 2004;49(9):1359-77. CrossRef PubMed
  14. Watts JA, Maiorano PC. Trace amounts of albumin protect gainst ischemia and reperfusion injury in isolated rat hearts. J Mol Cell Cardiol. 1999; 31:1653-62. CrossRef PubMed
  16. Lee EJ, Lee S-A, Kim J. The effect of human serum albumin on the extended storage of human oral keratinocyte viability under mild hypothermia. Cryobiol. 2005; 50:103–11. CrossRef PubMed
  18. Puehler T, Gleich O, Schopka S, Rupprecht L, S. Hirt, Schmid C, et al. Impact of normothermic perfusion and protein supplementation on human endothelial cell function during organ preservation. Ann Thorac Surg. 2010;89:512-21. CrossRef PubMed
  20. Guiberta EE, Petrenkob AY, Balabana CL, Somovb AY, Rodrigueza JV, Fullerc BJ. Organ preservation: current concepts and new strategies for the next decade. Transfus Med Hemother. 2011;38:125-42. CrossRef PubMed PubMedCentral
  22. Bedaiwy MA, Shahin AY, Falcone T. Reproductive organ transplantation: advances and controversies. Fertil Steril. 2008;90(6):2031-55. CrossRef PubMed
  24. Blatt J. Pregnancy outcome in long-term survivors of childhood cancer. Med Pediatr Oncol. 1999;33(1):29-33. CrossRef  
  25. Donnez J, Jadoul P, Langendonckt A, Eyck A, Dolmans M. Ovarian tissue cryopreservation and transplantationin cancer patients. Best Pract Res Clin Obstet Gynaecol. 2010;24:87-100. CrossRef PubMed
  27. Boiso I, Veiga A, Tresserra F, Grases P, Barri PN, Dexeus S. Ovarian tissue freezing, a promising method for fertility preservation. Ref Gynecol Obstet. 2001;8:448-52.
  29. Lilli R. Patogistologicheskaya tekhnika i prakticheskaya gistokhimiya. M.: Mir, 1969. 645 p
  31. Songsasen N, Fickes A, Pukazhenthi BS, Wildt DE. Follicular morphology, oocyte diameter and localisation of fibroblast growth factors in the domestic dog ovary. Reprod Domest Anim. 2009;44(2):65-70. CrossRef PubMed PubMedCentral
  33. Maxwell SJ, Lip GYH. Reperfusion injury: a review of the pathophysiology, clinical manifestations and therapeutic options. Int J Cardiol. 1997;58:95-117. CrossRef  
  34. Jennings RB, Schaper J, Hill ML, Steenbergen C, Reimer KA. Effect of reperfusion late in the phase of reversible ischemic injury.changes in cell volume, electrolytes, metabolites, and infrastructure. Circ Res. 1985;56:262-78. CrossRef PubMed
  36. McCarthy J.V., Cotter T.G. Cell shrinkage and apoptosis: a role for potassium and sodium ion effux. Cell Death and Different. 1997;4:756-70. CrossRef PubMed
  38. Redondo J, Pacheco ME, Manso AM, Salaices M, Mar?n J. Cell volume and ionic transport systems after cold preservation of coronary endothelial cells. Ann Thorac Surg. 2001;71:1290-5. CrossRef  
  39. Lang F, Ritter M, Gamper N, Huber S, Fillon S, Tanneur V, et al. Cell volume in the regulation of cell proliferation and apoptotic cell death. Cell Physiol Biochem. 2000;10: 417-28. CrossRef PubMed
  41. Maeno E, Ishizaki Y, Kanaseki T, Hazama A, Okada Y. Normotonic cell shrinkage because of disordered volume regulation is an early prerequisite to apoptosis. Proc Natl Acad Sci USA. 2000;97(17):9487-92. CrossRef PubMed PubMedCentral
  43. Okada Y, Maeno E, Shimizu T, Dezaki K, Wang J, Morishima S. Receptor-mediated control of regulatory volume decrease (RVD) and apoptotic volume decrease (AVD). J Physiol. 2001;532(1):3-16. CrossRef PubMed PubMedCentral
  45. Yu SP, Choi DW. Ions, cell volume, and apoptosis. Proc Natl Acad Sci USA. 2000;97(17):9360-2. CrossRef  
  46. Lang F. Mechanisms and significance of cell volume regulation. J Am Coll Nutr. 2007 Oct;26(5):613-623. CrossRef  
  47. Lang F, Uhlemann AC, Lepple-Wienhues A, Szabo I, Siemen D, Nilius B, et al. Cell volume regulatory mechanisms in apoptotic cell death. Herz. 1999;24(3):232-5. CrossRef PubMed
  49. Wyllie AH. Apoptosis: cell death in tissue. Reg J Path. 1987;153(3):313-6. CrossRef PubMed
  51. Bortner CD, Cidlowski JA. Caspase independent/dependent regulation of K+, cell shrinkage, and mitochondrial membrane potential during lymphocyte apoptosis. J Biol Chem. 1999;274:21953-62. CrossRef  
  52. Gomez-Angelats M, Bortner CD, Cidlowski JA. Cell volume regulation in immune cell apoptosis. Cell Tissue Res. 2000;301;33-42. CrossRef PubMed
  54. Xiao AY, Wei L, Xia S, Rothman S, Yu SP. Ionic mechanism of ouabain-induced concurrent apoptosis and necrosis in individual cultured cortical neurons. J Neurosci. 2002;22(4):1350-62. CrossRef PubMed
  56. Ekhterae D, Platoshyn O, Zhang S, Remillard CV, Yuan JX-J. Apoptosis repressor with caspase domain inhibits cardiomyocyte apoptosis by reducing K currents. Am J Physiol Cell Physiol. 2003;284:1405-10. CrossRef PubMed
  58. Lionetto MG, Giordano ME, Calisi A, Caricato R, Hoffmann E, Schettino T. Role of BK channels in the apoptotic volume decrease in native eel intestinal cells. Cell Physiol Biochem. 2010;25(6):733-44. CrossRef PubMed
  60. Perez GI, Maravei DV, Trbovich AM, Cidlowski JA, Tilly JL, Hughes FM. Identification of potassium-dependent and -independent components of the apoptotic machinery in mouse ovarian germ cells and granulosa cells. – Biol Reprod. 2000;63:1358-69. CrossRef PubMed

© National Academy of Sciences of Ukraine, Bogomoletz Institute of Physiology, 2014-2024.