Dynamics of structural change of ovarian tissue under ischemia
Bozhkova IuO, Kiroshka VV, Havas AA, Bondarenko VA
Institute for Problem of Cryobiology and Criomedicine ofNAS of Ukraine, Kharkiv, Ukraine
DOI: https://doi.org/10.15407/fz60.05.089
Abstract
In this work it was investigated the dependence of the correlation
of folliculogenesis and endocrine function of ovarian
tissue on the degree of structural damage and oocyte volume
changes during ischemia. It was shown that after 2 hours of
ischemia at 37 °C the morphological transformation of the
structural components of the ovarian tissue were reversible.
In case of restoration of blood flow conditions by heterotopic
transplantation estradiol level of animals was 25,9 ± 5,18 pg/
ml, progesterone - 18,48 ± 3,69 ng/ml, significantly higher than
the castrated animals-recipients. Supplement of the incubation
medium by the fetal bovine serum lead to decreasing by 5-7
% in the volume of oocytes of growing follicles during in
ischemia and reduced steroidogenic function of ovarian tissue
after perfusion. Increased time of the ischemia up to 4 hours
was founded in irreversible morphological transformation,
reduce the volume of oocytes by 40 % and the formation of
sclerosed tissue after transplantation of the ischemic fragments
of ovarian tissue.
Keywords:
ovarian tissue, ischemic damage, endocrine function,the volume of oocyte.
References
- Grace PA. Ischemia-reperfusion injury. Brit J Surg. 1994;81:631-41.
CrossRef
- Dorweiler B, Pruefer D, Andrasi TB, Maksan SM, Schmiedt W, Neufang A, et al. Ischemia-Reperfusion Injury. Eur J Trauma Emerg Surg . 2007;33(6):600-12.
CrossRef
PubMed
- Kerrigan CL, Stotland MA. Ischemia reperfusion injury: a review. Microsurgery. 1993;14:165-75.
CrossRef
- Piper HM, Meuter K, Schafer C. Cellular mechanisms of ischemia-reperfusion injury. Ann Thorac Surg. 2003;75:644-8.
CrossRef
- Fu H, Xu H, Chen H, Li Y, Li W, Zhu Q, et al. Inhibition of Glycogen Synthase Kinase 3 Ameliorates Liver Ischemia/Reperfusion Injury via an Energy-dependent Mitochondrial Mechanism. J Hepatol. 2014 May;23:358- 4.
CrossRef
- Ozturk H, Ozturk H, Terzi EH, Ozgen U, Duran A, Uygun I. Protective effects of rosmarinic acid against renal ischaemia/reperfusion injury in rats. J Pak Med Assoc. 2014 Mar;64(3):260-5.
PubMed
- Alvarez P, Tapia L, Mardones LA, Pedemonte JC, Farias JG, Castillo RL. Cellular mechanisms against ischemia reperfusion injury induced by the use of anesthetic pharmacological agents. Chem Biol Interact. 2014 May 13;218:89-98.
CrossRef
PubMed
- Reilly PM, Schiller HJ, Bulkley GB. Pharmacologic approach to tissue injury mediated by free radicals and other reactive oxygen metabolites. Amer J Surg. 1991;161:488-504.
CrossRef
- Mallick IH, Yang W, Winslet MC, Seifalian AM. Ischemia– reperfusion injury of the intestine and protective strategies against injury. Digestive Diseases and Sciences. 2004;49(9):1359-77.
CrossRef
PubMed
- Watts JA, Maiorano PC. Trace amounts of albumin protect gainst ischemia and reperfusion injury in isolated rat hearts. J Mol Cell Cardiol. 1999; 31:1653-62.
CrossRef
PubMed
- Lee EJ, Lee S-A, Kim J. The effect of human serum albumin on the extended storage of human oral keratinocyte viability under mild hypothermia. Cryobiol. 2005; 50:103–11.
CrossRef
PubMed
- Puehler T, Gleich O, Schopka S, Rupprecht L, S. Hirt, Schmid C, et al. Impact of normothermic perfusion and protein supplementation on human endothelial cell function during organ preservation. Ann Thorac Surg. 2010;89:512-21.
CrossRef
PubMed
- Guiberta EE, Petrenkob AY, Balabana CL, Somovb AY, Rodrigueza JV, Fullerc BJ. Organ preservation: current concepts and new strategies for the next decade. Transfus Med Hemother. 2011;38:125-42.
CrossRef
PubMed PubMedCentral
- Bedaiwy MA, Shahin AY, Falcone T. Reproductive organ transplantation: advances and controversies. Fertil Steril. 2008;90(6):2031-55.
CrossRef
PubMed
- Blatt J. Pregnancy outcome in long-term survivors of childhood cancer. Med Pediatr Oncol. 1999;33(1):29-33.
CrossRef
- Donnez J, Jadoul P, Langendonckt A, Eyck A, Dolmans M. Ovarian tissue cryopreservation and transplantationin cancer patients. Best Pract Res Clin Obstet Gynaecol. 2010;24:87-100.
CrossRef
PubMed
- Boiso I, Veiga A, Tresserra F, Grases P, Barri PN, Dexeus S. Ovarian tissue freezing, a promising method for fertility preservation. Ref Gynecol Obstet. 2001;8:448-52.
- Lilli R. Patogistologicheskaya tekhnika i prakticheskaya gistokhimiya. M.: Mir, 1969. 645 p
- Songsasen N, Fickes A, Pukazhenthi BS, Wildt DE. Follicular morphology, oocyte diameter and localisation of fibroblast growth factors in the domestic dog ovary. Reprod Domest Anim. 2009;44(2):65-70.
CrossRef
PubMed PubMedCentral
- Maxwell SJ, Lip GYH. Reperfusion injury: a review of the pathophysiology, clinical manifestations and therapeutic options. Int J Cardiol. 1997;58:95-117.
CrossRef
- Jennings RB, Schaper J, Hill ML, Steenbergen C, Reimer KA. Effect of reperfusion late in the phase of reversible ischemic injury.changes in cell volume, electrolytes, metabolites, and infrastructure. Circ Res. 1985;56:262-78.
CrossRef
PubMed
- McCarthy J.V., Cotter T.G. Cell shrinkage and apoptosis: a role for potassium and sodium ion effux. Cell Death and Different. 1997;4:756-70.
CrossRef
PubMed
- Redondo J, Pacheco ME, Manso AM, Salaices M, Mar?n J. Cell volume and ionic transport systems after cold preservation of coronary endothelial cells. Ann Thorac Surg. 2001;71:1290-5.
CrossRef
- Lang F, Ritter M, Gamper N, Huber S, Fillon S, Tanneur V, et al. Cell volume in the regulation of cell proliferation and apoptotic cell death. Cell Physiol Biochem. 2000;10: 417-28.
CrossRef
PubMed
- Maeno E, Ishizaki Y, Kanaseki T, Hazama A, Okada Y. Normotonic cell shrinkage because of disordered volume regulation is an early prerequisite to apoptosis. Proc Natl Acad Sci USA. 2000;97(17):9487-92.
CrossRef
PubMed PubMedCentral
- Okada Y, Maeno E, Shimizu T, Dezaki K, Wang J, Morishima S. Receptor-mediated control of regulatory volume decrease (RVD) and apoptotic volume decrease (AVD). J Physiol. 2001;532(1):3-16.
CrossRef
PubMed PubMedCentral
- Yu SP, Choi DW. Ions, cell volume, and apoptosis. Proc Natl Acad Sci USA. 2000;97(17):9360-2.
CrossRef
- Lang F. Mechanisms and significance of cell volume regulation. J Am Coll Nutr. 2007 Oct;26(5):613-623.
CrossRef
- Lang F, Uhlemann AC, Lepple-Wienhues A, Szabo I, Siemen D, Nilius B, et al. Cell volume regulatory mechanisms in apoptotic cell death. Herz. 1999;24(3):232-5.
CrossRef
PubMed
- Wyllie AH. Apoptosis: cell death in tissue. Reg J Path. 1987;153(3):313-6.
CrossRef
PubMed
- Bortner CD, Cidlowski JA. Caspase independent/dependent regulation of K+, cell shrinkage, and mitochondrial membrane potential during lymphocyte apoptosis. J Biol Chem. 1999;274:21953-62.
CrossRef
- Gomez-Angelats M, Bortner CD, Cidlowski JA. Cell volume regulation in immune cell apoptosis. Cell Tissue Res. 2000;301;33-42.
CrossRef
PubMed
- Xiao AY, Wei L, Xia S, Rothman S, Yu SP. Ionic mechanism of ouabain-induced concurrent apoptosis and necrosis in individual cultured cortical neurons. J Neurosci. 2002;22(4):1350-62.
CrossRef
PubMed
- Ekhterae D, Platoshyn O, Zhang S, Remillard CV, Yuan JX-J. Apoptosis repressor with caspase domain inhibits cardiomyocyte apoptosis by reducing K currents. Am J Physiol Cell Physiol. 2003;284:1405-10.
CrossRef
PubMed
- Lionetto MG, Giordano ME, Calisi A, Caricato R, Hoffmann E, Schettino T. Role of BK channels in the apoptotic volume decrease in native eel intestinal cells. Cell Physiol Biochem. 2010;25(6):733-44.
CrossRef
PubMed
- Perez GI, Maravei DV, Trbovich AM, Cidlowski JA, Tilly JL, Hughes FM. Identification of potassium-dependent and -independent components of the apoptotic machinery in mouse ovarian germ cells and granulosa cells. – Biol Reprod. 2000;63:1358-69.
CrossRef
PubMed
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