The role of mitochondrial uniporter in calcium-homeostasis of the exorbital lacrimal gland secretory cells
Kotliarova AB1,2, Merlavs'kyĭ VM1, Dorosh OM1, Man'ko VV1
- Ivan Franko National University of L`viv, Ukraine
- Bogomoletz Institute of Physiology NAS of Ukraine, Kyiv, Ukraine
DOI: https://doi.org/10.15407/fz60.05.073
Abstract
The role of mitochondrial calcium-uniporter in calciumhomeostasis
maintenance and correlations of calciumuniporter
with other calcium-transport systems of the
rat exorbital lacrimal gland secretory cells were studied.
The experiments were performed on intact and digitoninpermeabilized
cells. The interdependence of calciumuniporter
and other calcium-transporting systems functioning
was estimated on the basis of additivity of their
inhibitors/agonists effects, which was accompanied with
a decrease in the Ca2+ content in the gland cells. It was
found that in conditions of simultaneously inhibition of
sarco endoplasmic reticulum „Ra2+-ATPase (SERCA) and
mitochondrial calcium-uniporter Ca2+ passively released
from different calcium stores, because the effects of these
calcium-transport systems inhibitors (thapsigargin and
ruthenium red, respectively) were additive. Similarly, the
processes of inositol-1,4,5-trisphosphate receptors (IP3Rs)
activation and Calcium-uniporter inhibition were additive.
In contrast, the effects of ryanodine and ruthenium red on
the Ca2+ content in cells were significantly non-additive.
In addition, ryanodine at concentrations 1.3 ѓК„M reduced
respiration rate of studied cells in dose-dependent manner,
and this effect was persisted at cells preincubation with
ruthenium red or tapsigargin. Thus, besides the activation
of ryanodine receptors (RyRs) in endoplasmic reticulum,
ryanodine inhibits Ca2+ influx to the mitochondrial matrix,
that was insensitive to ruthenium red.
Key words: Calcium-uniporter of mitochondria, ruthenium
red, sarcoendoplasmic reticulum „Ra2+-ATPase (SERCA),
inositol-1,4,5-trisphosphate receptors (IP3Rs), ryanodine
receptors (RyRs), lacrimal glands, secretory cells.
Keywords:
Calcium-uniporter of mitochondria, rutheniumred, sarcoendoplasmic reticulum Сa2+-ATPase (SERCA),inositol-1,4,5-trisphosphate receptors (IP3Rs), ryanodinereceptors (RyRs), lacrimal glands, secretory cells.
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