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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2014; 60(5): 73-81

The role of mitochondrial uniporter in calcium-homeostasis of the exorbital lacrimal gland secretory cells

Kotliarova AB1,2, Merlavs'kyĭ VM1, Dorosh OM1, Man'ko VV1

  1. Ivan Franko National University of L`viv, Ukraine
  2. Bogomoletz Institute of Physiology NAS of Ukraine, Kyiv, Ukraine


The role of mitochondrial calcium-uniporter in calciumhomeostasis maintenance and correlations of calciumuniporter with other calcium-transport systems of the rat exorbital lacrimal gland secretory cells were studied. The experiments were performed on intact and digitoninpermeabilized cells. The interdependence of calciumuniporter and other calcium-transporting systems functioning was estimated on the basis of additivity of their inhibitors/agonists effects, which was accompanied with a decrease in the Ca2+ content in the gland cells. It was found that in conditions of simultaneously inhibition of sarco endoplasmic reticulum „Ra2+-ATPase (SERCA) and mitochondrial calcium-uniporter Ca2+ passively released from different calcium stores, because the effects of these calcium-transport systems inhibitors (thapsigargin and ruthenium red, respectively) were additive. Similarly, the processes of inositol-1,4,5-trisphosphate receptors (IP3Rs) activation and Calcium-uniporter inhibition were additive. In contrast, the effects of ryanodine and ruthenium red on the Ca2+ content in cells were significantly non-additive. In addition, ryanodine at concentrations 1.3 ѓК„M reduced respiration rate of studied cells in dose-dependent manner, and this effect was persisted at cells preincubation with ruthenium red or tapsigargin. Thus, besides the activation of ryanodine receptors (RyRs) in endoplasmic reticulum, ryanodine inhibits Ca2+ influx to the mitochondrial matrix, that was insensitive to ruthenium red. Key words: Calcium-uniporter of mitochondria, ruthenium red, sarcoendoplasmic reticulum „Ra2+-ATPase (SERCA), inositol-1,4,5-trisphosphate receptors (IP3Rs), ryanodine receptors (RyRs), lacrimal glands, secretory cells.

Keywords: Calcium-uniporter of mitochondria, rutheniumred, sarcoendoplasmic reticulum Сa2+-ATPase (SERCA),inositol-1,4,5-trisphosphate receptors (IP3Rs), ryanodinereceptors (RyRs), lacrimal glands, secretory cells.


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