Phase changes of energy metabolism during adaptation to immobilization stress
Portnichenko VI1,2, Nosar VI1, Honchar OO1, Opanasenko HV1, Hlazyrin ID1, Man'kovs'ka IM1
- Bogomoletz Institute of physiology NAS of Ukraine, Kyiv, Ukraine
- International center of the astronomer and the medical-ecologist. Researches of the National Academy of Sciences of Ukraine, Kiev, Ukraine
The properties of ДpH-induced Ca2+-transport from isolated
rat myometrium mitochondria was investigated. Ca2+-accumulation
was carried out in the presence of Mg-ATP2- and
succinate. Transport of Ca2+ recorded using Ca2+-sensitive
fluorescent probe Fluo-4 AM. It is shown that acidification of
extramitochondrial medium is accompanied by stimulation of
Ca2+ release from mitochondria. This process is insensitive
to the tetraphenylphosphonium which is relatively specific
Na+–Ca2+-exchanger inhibitor of mitochondrial inner membrane,
but inhibited in the presence of monoclonal antibodies
directed against the protein LETM1 (Anti-LETM1). LETM1
protein in some tissues is the molecular basis of the H+–Ca2+-
exchanger functioning on mitochondria. It was found that the
H+–Ca2+-exchanger is stimulated by 100 мM amiloride (diuretic)
and inhibited by Mg ions in milimolar concentrations.
The transport system was completely resistant to the action
of nitric oxide (sodium nitroprusside and sodium nitrite), but
was stimulated by macrocyclic compounds of Calixarenes
(C-97 and C-99) in submicromolar concentrations. Thus, the
mitochondria of rat myometrium probably not have a system
of Na+–Ca2+-exchanger, and provide the maintenance of the
matrix Ca2+-homeostasis with H+–Ca2+-exchanger. Since
the transport system high affinity activated by Calixarenes,
further investigation of the influence of these compounds on
the transport process makes promising.
Key words: H+–Ca2+-exchanger, LETM1, mitochondria,
H+–Ca2+-exchanger, LETM1, mitochondria,Calixarenes, myometrium.
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