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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2014; 60(5): 3-13

Induction of nitrosative stress in mitochondria of rats hearts in experimental ischemia-reperfusion of the brain and its correction by ecdysterone

Sharipov RR, Kotsiuruba AV, Kop"iak BS, Sahach VF.

    O.O. Bogomoletz Institute of Physiology, National Academyof Sciences of Ukraine, Kyiv, Ukraine
DOI: https://doi.org/10.15407/fz60.05.003


Abstract

On the model of focal ischemia–reperfusion of the brain investigated the induction of nitrosative stress in mitochondria of rats hearts and possible mechanisms of protective action of ecdysterone. It is shown that focal ischemia–reperfusion of the brain induced in myocardial mitochondria the activation of constitutive and inducible de novo synthesis of NO by oxidation of L-arginine and not oxidative synthesis of NO through the recovery of oxidized stable metabolites of NO. Strong evidence of induction of nitrosative stress in heart mitochondria by focal ischemia - reperfusion of the brain, was a significant increase in mitochondrial pool of nitrat- and nitrite- anions and pools of nitrosothiols, that is proof of the formation and decay of peroxynitrite - a key marker of nitrosative stress. Also was observed increase in heart mitochondria by focal ischemia–reperfusion of the brain, content key regulator of de novo synthesis of NO - hydrogen sulfide and activity of inducible arginase II and, as a result, the pool of carbamide, which is also a regulator of the synthesis of NO. Previous introduction for animals herbal extract Serratsula coronata, enriched ecdysterone, reduces induction nitrosative stress in mitochondria of rats hearts under conditions of focal ischemia–reperfusion of the brain.

Keywords: mitochondria, rats, heart, ischemia-reperfusion, brain, ecdysterone

Full text: (PDF, in Ukrainian)

References

  1. Zozulya IS, Moshenska OP. The acute period of ischemic stroke: a modern view on the problem Ukr Med J. 2009; 4: 67-73 [Ukrainian].
    2.  
  2. Hyvrinen M, Qiao Q, Tuomilehto J. et al. The difference between acute coronary heart disease and ischaemic stroke risk with regard to gender and age in Finnish and Swedish population Intern. J of Stroke. 2010; 5 : 152-56. CrossRef PubMed
    3.  
  3. Johnson RH, Lambie DG, Spalding JMK. Neurocardiology: The Interrelationships Between Dysfunction in the Nervous and Cardiovascular System. London, England: WB Saunders; 1984: 66-70.
    4.  
  4. Zhdanov GN, Gerasimov MM. Studying the content of proinflammatory and anti-inflammatory cytokines in the serum of patients with acute phase of ischemic stroke. Cytokines and Inflam. 2006;5(1):27-30 [Russian].
    5.  
  5. di Villa Bianca R, Sorrentino R, Imbimbo C. et al. Sphingosine 1-phosphate induces endothelial nitric -oxide synthase activation through phosphorylation in human corpus cavernosum J Pharmacol Exp. 2006; 316(2): 703-08.
    6.  
  6. Kotsiuruba AV, Tuhanova AV, Bukhanevich OM, Tarakanov SS. Mechanisms of the early effect of biologically active hydroxysterols: calcitriol and ecdysterone. Identification of sphingomyelin as the effector mechanism of the early effect Ukr Biokhim Zh. 1995;67(2):53-8 [Ukrainian]. PubMed
    7.  
  7. Shchokina E. G., Study of the cardioprotective features of the recombinant antagonist interleukin-1 receptors under conidtions of the adrenal myocarditis in rats. Ukr J of Biopharm. 2012; (5-6):47-51 [Ukrainian].
    8.  
  8. Sharinov RR, Kotsiuruba AV, Kopyak BS, Sagach VF. Induction of oxidative stress in heart mitochondria by focal ischemia – reperfusion brain and protective effect of ecdysterone. Fiziol.Zh. 2014;60(3):11-7 [Ukrainian]. CrossRef  
  9. Sojitra B, Bulani Y, Putcha UK, Kanwal A, Gupta P, et al. Nitric oxide synthase inhibition abrogates hydrogen sulfide-induced cardioprotection in mice Mol Cell Biochem. 2012;360: 61-69. CrossRef PubMed
    10.  
  10. Ali MY, Ping CY, Mok YY, Ling L, Whiteman M, Bhatia M, Moore PK. Regulation of vascular nitric oxide in vitro and in vivo; a new role for endogenous hydrogen sulphide? Brit J Pharmacol. 2006;149: 625-34. CrossRef PubMed PubMedCentral
    11.  
  11. King DL, Polhemus DJ, Bsushan SJ et al. Hydrogen sulfide cytopritective signaling is endothelial nitric oxide synthase-nitric oxide dependent PNAS. 2014; 111(8): 3182-87. CrossRef PubMed PubMedCentral
    12.  
  12. Sagach VF, Korkach YP, Kotsyuruba AV et al. Inhibition of opening mitochondrial pore by ecdysterone in the heart of old rats Fiziol. Zh. 2008;54(4):3-10 [Ukrainian].
    13.  
  13. Korkach JuP, Rudyk OV, Kotsuruba AV, Prysyazhna OD, Sagach VF. The nitric oxide and superoxide synthesis in protective action of ecdysteron in mitochondrias of rats with streptozotocin-induced diabetes Fiziol. Zh. 2007;53(5):1- 6 [Ukrainian].
    14.  
  14. Traystman RJ. Animal models of focal and global cerebral ischemia. ILARJ.2003;44(2):85-95. CrossRef  
  15. Salter M, Knowles RG, Moncada S. Widespread tissue distribution, species and changes in activity of Ca2+- dependent and Ca2+-independent nitric oxide syntases FEBS Lett. 1991;291(1):145-49. CrossRef  
  16. Chin SY, Pandey KN, Shi SJ. Increased activity and expression of Ca2+-dependent NOS in renal cortex of ANG Il-infused hypertensive rats Am J Physiol. 1999;277(5):797-84.
    17.  
  17. Boyde JR, Rahmotullah M. Optimization of conditions for the colorimetric determination of citrulline, using diacetil monoxime Anal. Biochem.1980; (107):424-31. CrossRef  
  18. Alikulov ZA, Lvov NP, Kretovich VL. Nitrate and nitrite reductase activity of milk Biochemistry. 1980;45(9):1714- 18 [Russian].
    19.  
  19. Shugalei VS, Kozina AS. Urea content and arginase activity in rat organs during acclimatization to cold Physiol. J. USSR. 1977; (8): 1199-02 [Ukrainian].
    20.  
  20. Green LC, Wagner DA, Glogowski J. et al. Analysis of nitrate, nitrite and [N] nitrate in biological fluids Anal Biochem. 1982;126 (1):131-38. CrossRef  
  21. Jsukahara H. Effect of NOS inhibitions on bone methabolizm in growing rats Am J. Physiol. 1996; (270)5: 840-45.
    22.  
  22. Gerdel D, Cederbaum AJ. Inhibition of the catalitic activity of alkoholdegydrogenase by NO is associated with S-nitrosylation and the release of zinc Biochemistry. 1996;35(50):16186-194. CrossRef PubMed
    23.  
  23. Hue Yan, Junbao Du, Chaoshu Tang. The possible role of hydrogen sulfide on the pathogenesis of spontaneous hypertension in rats. Biochem and Biophys Res Commun. 2004:313(1):22-27. CrossRef PubMed
    24.  
  24. Lowery OH, Rosebroughh NI, Farr AL, Randall RI. Protein measurement with the Folin phenol reagent JBiolChem. 1951; 193(1): 265-75.
    25.  
  25. Sagach VF, Korkach YuP, Kotsuruba AV, Prysyazhna OD. The inhibition of oxidative and nitrosative stresses by ecdysterone as the mechanisms of its cardio- and vasoprotective action at type Fiziol. Zh. 2008;54(5):1-9 [Ukrainian].
    26.  
  26. Rhee HJ, Kim EJ, Lee JK. Physiological polyamines:simple primordial stress molecules J Cell Mol Med. 2007;11(4):685-703. CrossRef PubMed PubMedCentral
    27.  
  27. Loke KE, Laycock SK. Mital S. et al. Nitric oxide modulates mitochondrial respitation in failing human heart Circulation. 1999;100 (12):1291-97. CrossRef PubMed
    28.  
  28. Reutov VP, Sorokina EG, Ohotyn VE, Kosytsyn NS. Cyclic conversion of nitric oxide in the body of mammals Science, M. 1997:158 [Russian].
    29.  
  29. Moibenko OO, Sagach VF, Tkachenko MM. et al. Fundamental mechanisms of action of nitric oxide on the cardiovascular system, as the basis of pathogenetic treatment of diseases Fiziol. Zh. 2004;50 (1): 11-30 [Ukrainian].
    30.  
  30. Lee C I, Lin X, Zweier J.L. Regulation of xanthine oxidase by nitric oxide and peroxynitrite// J Biol Chem. 2000;275(3): 9369–76. CrossRef PubMed
    31.  
  31. Kihara M, Schmelzer JD, Poduslo JF. et al. Aminoguanidine effects on nerve blood flow, vascular permeability, electrophysiology and oxygen free radicals Proc Natl Acad Sci USA. 1991;88(14):6107-11. CrossRef PubMed PubMedCentral
    32.  
  32. Strutynska NA, Semenykhina OM, Shorna SV, Vavilova HL, Sagach VF. Hydrogen sulfide inhibits Ca(2+)- induced mitochondrial permeability transition pore opening in adult and old rat heart Fiziol. Zh. 2011;57(6):3-14 [Ukrainian].
    33.  

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