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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2014; 60(5): 3-13

Induction of nitrosative stress in mitochondria of rats hearts in experimental ischemia-reperfusion of the brain and its correction by ecdysterone

Sharipov RR, Kotsiuruba AV, Kop"iak BS, Sahach VF.

    O.O. Bogomoletz Institute of Physiology, National Academyof Sciences of Ukraine, Kyiv, Ukraine


On the model of focal ischemia–reperfusion of the brain investigated the induction of nitrosative stress in mitochondria of rats hearts and possible mechanisms of protective action of ecdysterone. It is shown that focal ischemia–reperfusion of the brain induced in myocardial mitochondria the activation of constitutive and inducible de novo synthesis of NO by oxidation of L-arginine and not oxidative synthesis of NO through the recovery of oxidized stable metabolites of NO. Strong evidence of induction of nitrosative stress in heart mitochondria by focal ischemia - reperfusion of the brain, was a significant increase in mitochondrial pool of nitrat- and nitrite- anions and pools of nitrosothiols, that is proof of the formation and decay of peroxynitrite - a key marker of nitrosative stress. Also was observed increase in heart mitochondria by focal ischemia–reperfusion of the brain, content key regulator of de novo synthesis of NO - hydrogen sulfide and activity of inducible arginase II and, as a result, the pool of carbamide, which is also a regulator of the synthesis of NO. Previous introduction for animals herbal extract Serratsula coronata, enriched ecdysterone, reduces induction nitrosative stress in mitochondria of rats hearts under conditions of focal ischemia–reperfusion of the brain.

Keywords: mitochondria, rats, heart, ischemia-reperfusion, brain, ecdysterone


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