Українська English

ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2014; 60(4): 97-104

The effect of altered oxygen partial pressure on the resisitance to hypoxia and expression of oxygen-sensitive genes in Drosophila melanogaster

Berezovs'kyĭ VIa, Chaka OH, Litovka IH, Levashov MI, Ianko RV

    O.O. Bogomoletz Institute of Physiology, National Academyof Sciences of Ukraine, Kyiv, Ukraine


As a result of resistance test to hypoxia of Drosophilas melanogaster of Оregon strain, we identified a high resistance (Group II) and low resistance (Group III) subpopulations of flies. Flies from groups II and III were incubated in a constant normobaric hypoxiа (Ро2=62-64 mm Hg) for 10 generations. A highly resistant group (Group IV) were exposed to a shortterm anoxia (Ро2=1,5 mm Hg, 5 min) every generation. Larvae from Groups II, III, and IV demonstrated significantly elevated levels of Sir and CG14740 expression. Larvae from Group II had a significantly higher expression of CG14740 compared to group III. The restitution time after exposure to anoxia was significantly reduced in Group II (on 31% of the control values) Our results suggest that long-term adaptation to low oxygen partial pressure of highly resistant Drosophila significantly reduces the time of restitution and increases the expression of Sir2 and CG14740 genes. Key words: hypoxia, drosophila, expression of genes Sir2, CG14740, PyK.

Keywords: hypoxia, drosophila, expression of genes Sir2,CG14740, PyK.


  1. Iyer N.V., Kotch L.E., Agani F., Leung S.W., Laughner E., Semenza G.L. Cellular and developmental control of O2 homeostasis by hypoxia-inducible factor 1?i. Genes Dev. 1998; 12 (2): 149 .162.
  3. Semenza G.L., Shimoda L.A., Prabhakar N. R. Regulation of gene expression by hypoxia-inducible factor 1. Novartis Found. Symp. 2006;272: 2 . 8.
  5. Shen C., Nettleton D., Jiang M., Kim S. K., Powell- Coffman J.A. Roles of the HIF-1 hypoxia-inducible factor during hypoxia response in Caenorhabditis elegans. J Biol chem. 2005; 280: 20580 .20588.
  7. Hubbi M.E., Hu H., Kshitiz, Gilkes D.M., Semenza G.L. Sirtuin-7 inhibits the activity of hypoxia-inducible factors. J Biol Chem. 2013; 288 (29):. 20768 . 20775.
  9. Laemmle A., Lechleiter A., Roh V., Schwarz C., Portman S., Furer C., Keogh A., Tschan M., Candinas D., Vorburger S.A., Stroka D. Inhibition of SIRT1 impairs the accumulation and transcriptional activity of HIF-1?i protein under hypoxic conditions. PLoS One. 2012; 7 (3): 33433 CrossRef PubMed PubMedCentral
  11. Feala J.D., Coquin L., Zhou D. Metabolism as means for hypoxia adaptation: metabolic profiling and flux balance analysis. BMC Systems biology. 2009; 3 (1): 91. CrossRef PubMed PubMedCentral
  13. Liu G., Roy J., Johnson E."@. Identification and function of hypoxia-response genes in Drosophila melanogaster. Physiol. Genomics. 2006; 25(1):134 –141. CrossRef PubMed
  15. Ma E., Xu T., Haddad G.G. Gene regulation by O2 deprivation: an anoxia-regulated novel gene in Drosophila melanogaster. Brain res. mol. brain res. 1999; 63: 217–224. CrossRef  
  16. Charette M., Darveau C., Perry S.F., Rundle H. Evolutionary consequences of altered atmospheric oxygen in Drosophila melanogaster. PLoS One. 2011;6(10): e26876 CrossRef PubMed PubMedCentral
  18. Zhao H.W., Haddad G.G., Jiang M., Kim S., Powell- Coffman A. Review: Hypoxic and oxidative stress resistance in Drosophila melanogaster. Placenta. 2011; 32 (2): 104 – 108. CrossRef PubMed PubMedCentral
  20. Zhou D., Xue J., Chen J., Morcillo P., Lambert J.D. Ehperimental selection for Drosophila survival in extremely low O2 environment . Plos One. 2007; 2 ( 5): e. 490.
  22. Zhou D., Xue J., Lai J.C. Schork N.J, White K.P., Haddad G.G. Mechanisms underlying hypoxia tolerance in Drosophila melanogaster: hairy as a metabolic switch PLoS Genet.2008; 4 (10): e1000221.
  24. Azad P., Haddad G.G. Survival in acute and severe low O2 environment: use of a genetic model system. Ann N Y acad sci. 2009; 1177: 39 – 47. CrossRef PubMed
  25. Berezovskyi V.A. Hypoxia: individual special features of reactivity. – Kyiv: Nauk. dumka, 1978. – 215 p. 
  26. Berezovskyi V.A. Patent for the useful model № 67049.Method of quantitative evaluation of intensivity of hypoxia influence. – 28.07.2011, Bul. № 2 
  28. Wheeler D., Barrett T., Yaschenko E. Database resources of the National Center for Biotechnology Information. Nucleic Acids Research. 2006; 34 (1): 173 – 180. CrossRef PubMed PubMedCentral
  29. Marygold S.J., Leyland P.C., Seal R.L., Goodman J.L., Thurmond J., Strelets V.B., Wilson R.J.; FlyBase consortium. FlyBase: improvements to the bibliography. Nucleic Acids Res. 2013;- Jan; 41 (Database issue) 
  31. Dioum E. M., Chen R., Alexander M. S., Zhang Q., Hogg R. T., Gerard R. D., Garcia J. A. Regulation of hypoxiainducible factor 2 alpha signaling by the stress-responsive deacetylase sirtuin 1.Science.2009; 324 (5932): 1289 – 1293. CrossRef PubMed
  33. Frankel S., Ziafazeli T., Rogina B. dSir2 and longevity in Drosophila. Exp gerontol. 2011; 46(5): 391–396. CrossRef PubMed PubMedCentral
  35. Ji-Hong L., Yoon-Mi L., Yang-Sook C. , Junjie C., Ja-Eun K., Jong-Wan P. Sirtuin 1 modulates cellular responses to hypoxia by deacetylating hypoxia-inducible factor 1?. Molecular cell. 2010; 38 (6): 864–878. CrossRef PubMed
  37. Rogina B., Helfand S.L. Sir2 mediates longevity in the fly through a pathway related to calorie restriction. Proc nat acad sci U S A. 2004; 101 (45):15998–16003. CrossRef PubMed PubMedCentral
  39. Hoffmann J., Romey R., Fink C., Yong L., Roeder T. Overexpression of Sir2 in the adult fat body is sufficient to extend lifespan of male and female Drosophila. Aging (Albany NY). 2013; 5 (4): 315 – 327. CrossRef PubMed PubMedCentral
  41. Chen N., Rinner O., Czernik D., Nytko K.J., Zheng D., Stiehl D.P., Zamboni N., Gstaiger M., Frei C. The oxygen sensor PHD3 limits glycolysis under hypoxia via direct binding to pyruvate kinase. Cell Res. 2011; 21(6): 983 – 986. CrossRef PubMed PubMedCentral
  42. Berezovskyi V.A. Natural and instrumental oroterapy. – Donetsk: Zaslavskyi, 2012 – 301 p.  
  44. Chaka E., Berezovskyi V.A., Levashov M.I., Litovka I.G., Janko R.V. Influence of normobaric hypoxia on the stability of Drosophila melanogaster to the stress // III International conference «Drosophila in experimental genetics and biology» Digest of scientific papers. – Kyiv. – 2012. – P. 44 – 46.

© National Academy of Sciences of Ukraine, Bogomoletz Institute of Physiology, 2014-2024.