Українська English

ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2014; 60(4): 87-96

Thyroxine caused modulation of dexamethasone effects on the skeletal muscle of white rats

Trush VV, Soboliev VI

    Donetsk National University, Ukraine


Experiments in situ on mature white female rats performed with the use of electrophysiological methods allowed to investigate the modulatory influence of thyroxin at the dose which does not cause the signs of hyperthyroidism (10 mkg/ kg), upon the manifestation of the dexamethasone effects on the functional state of the anterior tibial muscle. It has been established that the chronic isolated application of dexamethasone was accompanied by reduction of the amplitude of muscle contraction (by 29.7–59.3 per cent after 10–50 days of the drug injection) and the weight of anterior tibial muscle (by 22.4–12.7 per cent after 10–60 days of the drug injection). Combination of thyroxin with dexamethasone smoothed the negative effects of the synthetic glucocorticoid upon the muscle contraction amplitude and even caused its increase (by 41.2–62.1 per cent after 20–60 days of injection of the pair of preparations), as well as prevented the reduction of the muscle weight. The isolated application of dexamethasone after the first 20 days of injections caused the decrease of the muscle speed that was confirmed through a lengthened control of the muscle active state duration (by 20.5 per cent) and the reduction of its single contraction development speed (by 45.3 per cent), as well as the decrease of frequency of muscle tetanization (to 12-20 imp/s against 26-28 imp/s in control). The application of thyroxine with dexamethasone shortened the active state of the muscle (by 19.3 per cent) and increased the speed of single contraction development (by 72.4), which remained throughout whole further period the preparations were injected. These observations favor for improvement of high-speed characteristics of the muscle under the influence of thyroxine. At the same time, during chronic injection of dexamethasone either alone or in combination with thyroxin, an increased muscle fatigue during the first 10–20 days has been observed.

Keywords: dexamethasone, thyroxine, muscle contraction,muscle contraction amplitude, muscle contraction speed,maximum steady working capacity of a muscle.


  1. Borisova EO. Clinical pharmacology of the parenteral forms of glucocorticoids. Med Act. 2007; 3: 17-24.
  3. Bowes SB, Jackson NC, Papachristodoulou D. Effect of corticosterone on protein degradation in isolated rat soleus and extensor digitorum longus muscles. J. Endocrinol. 1996; 3: 501-507. CrossRef  
  4. Brodie C, Sampson SR. Characterization of thyroid hormone effects on membrane potential and Na-K pump in cultured rat skeletal myotubes. Neuromuscular Function. Amsterdam etc. 1989.
  6. Callozzo VJ, Baldwin KM. The influence of hyperthyroidism on maximal shortening velocity of slow and fast skeletal muscle. FASEB J. 1990; 1: 815.
  8. Caroccia L, Williams DA, Wrigth A. Effects of thyroid and parathyroid hormones on muscular activity. Proc. Austral. Physiol. and Pharmacol. Soc. 1988; 19-71.
  10. Connelly TJ, Hayek R, Sukhareva M. L-thyroxine activates the intracellular Ca2+ release channel of skeletal muscle sarcoplasmic reticulum. Biochem. Mol. Biol. Int. 1994; 32 (3): 441-448. PubMed
  12. Davis PJ, Leonard JL, Davis FB. Mechanisms of nongenomic actions of thyroid hormone. Front. Neuroendocrinol. 2008; 29: 211-218. CrossRef PubMed
  14. Gardahaut ME, Fontainne-Perus J, Rouaud T, Bandman E, Ferrand R. Developmental modulation of myosin expression by thyroid hormone in avian skeletal muscle. Development. 1992; 115 (1): 1121-1131. PubMed
  16. Gloss B, Villegas S, Villarreal FJ, Moriscot A, Dillmann WH. Thyroid hormone-induced stimulation of the sarcoplasmic reticulum Ca2+-ATPase gene is inhibited by LIF and IL-6. Am J Physiol. 2000; 278 (4): E738-E743. CrossRef  
  17. Guid book to experimental (preclinical) studying of new pharmacological substances (under the editorship of Fisenko V.P.). Moscow: Ministry of Health of the Russian Federation, RF ZAO «IIA «Remedium»»; 2000.
  19. Harrison AP, Clausen T. Thyroid hormone-induced upregulation of Na+ channels and Na+-K+ pumps: implications for contractility. Am J Physiol. 1998; 274 (5): R864-R867. CrossRef  
  20. Hulbert AJ. Thyroid hormones and their effects: a new perspective. Biol. Rev. 2000; 75: 519-631. CrossRef PubMed
  22. Kaasik P, Seene T, Umnova M. The mechanism of action of glucocorticoids in the rat skeletal muscle. Balt. J. Lab. Anim. Sci. 2000; 3-4: 185-193.
  24. Mac-Comas AJ. Skeletal muscles (structure and functions). Kiev: Olympic literature; 2001.
  26. Marchal S, Cassar-Malek I, Magaud JP, Rouault JP, Wrutniak C, Carello G. Stimulation of avian myoblast differentiation by triiodothyronine: possible involvement of the cAMP pathway. Exp Cell Res. 1995; 220 (1): 1-10. CrossRef PubMed
  28. Poletayev GI. Humoral regulation of a neuromuscular transmission and neurotrophic control of skeletal muscular fiber. Kazan Med J. 2001; 5: 321-325.
  30. Sampson SR, Bannett RR, Shainberg A. Effects of thyroxine on transmembrane resting potentials of skeletal muscle cells in culture. J. Neurosci. Res. 1982; 8 (4): 595-601. CrossRef PubMed
  32. Tata JR. Looking for the mechanism of action of thyroid hormone. Journal of Thyroid Research. 2011; 2011 [Electronic resource]. Access mode journals/jtr/2011/730630//
  34. Teppermen J, Teppermen H. Physiology of metabolism and endocrine system: the translate from English. Moscow: Peace; 1989.
  36. Usova NN. Iodinated thyroid hormones and its influence on nervous system. Med News. 2012; 4: 1-15.
  38. Yen PM. Physiological and Molecular Basis of Thyroid Hormone Action. Physiol Rev. 2001; 81 (3): 1097-1142. CrossRef PubMed

© National Academy of Sciences of Ukraine, Bogomoletz Institute of Physiology, 2014-2024.