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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2014; 60(4): 70-79


Effects of decarboxylated L-arginine on morphofunctional characteristics of the erythron in experimental diabetes mellitus in rats

Ferents IV, Brodiak IV, Liuta MIa, Kulachkovs'kyĭ OR, Sybirna NO

    Ivan Franko National University of Lviv, Ukraine
DOI: https://doi.org/10.15407/fz60.04.070

Abstract

This paper reports the results of a study of the impact of the agmatine treatment on erythrocyte resistance to acid hemolytic, reticulocyte count and reticulocyte production index, erythrocyte surface architectonics in streptozotocininduced diabetic rats. Our results indicate that treatment of diabetic rats with agmatine causes a suppression of erythropoiesis and increases the resistance of erythrocytes against HCl-induced hemolysis. It was shown a 10% increase in the number of young red blood cells and 35% reduction in the number of structurally transformed erythrocytes that are capable of restoring normal biconcave disc shape under physiological condition. Our data demonstrate an improvement of morphofunctional state of red blood cells in diabetes and reflect the positive effect of agmatine treatment on erythrone due to the glucose-lowering action.

Keywords: red blood cells, agmatine, experimental diabetesmellitus.

References

  1. Vydyborets SV. Erythrocytic changes in diabetes mellitus (a review of the literature). Vrach Delo. 1990 Feb;(2):56- 61. (Russian). Tsirkin VI, Dvoryanskiy SA, Troshkina NA. Red blood cell: structure and function of its membrane. Vyatskiy med vestnik. 2007;(2):32-40. (Russian).
  2. Tsirkin VI, Dvoryanskiy SA, Troshkina NA. Red blood cell: structure and function of its membrane. Vyatskiy med vestnik. 2007;(2):32-40. [Russian].
  3. Katyuhin LN. Rheological properties of erythrocytes. Modern methods of investigation. Ros Fiziol Zh im. I.M. Sechenova. 1995; 81(6):122-9. (Russian).
  4.  
  5. Berezina TL, Zaets SB, Morgan C, Spillert CR, Kamiyama M, Spolarics Z, Deitch EA, Machiedo GW. Influence of storage on red blood cell rheological properties. J Surg Res. 2002 Jan;102(1):6-12. CrossRef PubMed
  6.  
  7. Kravets EB, Ryazantseva NV, Jakovleva NM. Features of microrheological properties of erythrocytes in vascular complications of type 1 diabetes mellitus. Sakharnyi diabet. 2005; (1):14-7. (Russian).
  8.  
  9. Sokolov EI, Zabotnov VI, Podachina SV, Baluda MV. Disturbances of rheological properties of the blood and disorders of lipid spectrum of membranes of redblood- cells in patients with diabetes. Kardiologia. 1996; 36(9):67-70. (Russian).
  10.  
  11. Lipunova EA, Tukin VN, Rezanova TA. Change of the hematology parameters of elderly people's with diabetes mellitus. Nauchnyie vedomosti BelGU. Ser. Estestvennyie nauki. 2007; 5(5):140-3. (Russian).
  12.  
  13. Novitskiy VV, Ryazantseva NV, Stepovaya EA, Fedorova TS, Kravets EB, Ivanov VV, Zhavoronok TV, Chasovskih NYu, Chudakova OM, Butusova VN, Yakovleva NM. Molecular abnormalities of erythrocyte membrane in the pathology of different genesis are typical reaction of the body: the contours of the problem. Byul. Sib. Med. 2006; (2):62-9. (Russian).
  14.  
  15. Rabini RA., Fumelli P, Staffolani R, Mazzanti L, Pugnaloni A, Biagini G, Faloia E, De Pirro R. Effects of diabetes mellitus on structural and functional properties of erythrocyte membranes. Membr Biochem. 1993 Apr- Jun;10(2):71-9. CrossRef PubMed
  16.  
  17. Singh M, Shin S. Changes in erythrocyte aggregation and deformability in diabetes mellitus: a brief review. Indian J Exp Biol. 2009 Jan;47(1):7-15. PubMed
  18.  
  19. Kuypers FA, Lewis RA., Hua M, Schott MA, Discher D, Ernst JD, Lubin BH. Detection of altered membrane phospholipid asymmetry in subpopulations of human red blood cells using fluorescently labeled annexin V. Blood. 1996 Feb 1; 87(3):1179-87. PubMed
  20.  
  21. Gitel'zon II, Terskov IA. The erythrogram as a method of clinical investigation of the blood. Krasnoyarsk: Scientific academy of USSR, Siberia department; 1959. 246 r. (Russian).
  22.  
  23. Sybirna NO, Burda VA, Chajka YaP. Metody doslidzhennya systemy krovi: Navchal'no-metodychnyj posibnyk Research Methods of Blood: Textbook. Vydavnychyj centr LNU imeni Ivana Franka - Publishing Center of Ivan Franko LNU, 2005. 100 r. (Ukrainian).
  24.  
  25. Ionov BV, Chernukh AM. Morphological differences between erythrocytes of arterial and venous rat blood revealed by scanning electron microscopy. Bul Exp Biol and Med. 1981 Dec; 92(12):749-51. (Russian). CrossRef  
  26. Novoderzhkina YK, Shishkanova ZG, Kozinets GI. Blood cell configuration and surface under normal and pathological conditions. Moscow: «Triada-farm»; 2004. 152 r. (Russian).
  27. Burda V, Byront N, Sybirnay N, Kleveta G. Investigation of erytrone functional state under the conditions of streptozotocin-induced diabetes. Vis L'viv Univ. Biology Series. 2002; 28:21-7. (Ukrainian).
  28. Bondar TP, Kozinets GI. Morphofunctional condition of the peripheral blood erythrocytes in late vascular complications of diabetes mellitus of type 2. Klin Lab Diagnost. 2002;(12):22-34. (Russian).
  29. Ferents IV, Brodyak IV, Lyuta MYa. Effect of agmatine on the blood parameters of rats in experimental diabetes mellitus. Studia Biologica. 2012; 6(3): 65-72. (Ukrainian).
  30.  
  31. Kurilovich SA, Kruchinina MV, Generalov VM, Bakirov TS, Rikhter VA, Semenov DV. Electrical parameters and structure of membranes of erythrocytes at diffuse liver diseases. Ros. Zhurn. Gastroenterol. Gepatol. Koloproktol. 2009; 19(2):30-6. (Russian).
  32.  
  33. Satriano J, Isome M, Casero RA Jr, Thomson SC, Blantz RC. Polyamine transport system mediates agmatine transport in mammalian cells. Am J Physiol Cell Physiol. 2001 Jul; 281(1):C329-34.
  34. Arndt MA, Battaglia V, Parisi E, Lortie MJ, Isome M, Baskerville C, Pizzo DP, Ientile R, Colombatto S, Toninello A, Satriano J. The arginine metabolite agmatine protects mitochondrial function and confers resistance to cellular apoptosis. Am J Physiol Cell Physiol. 2009 Jun;296(6):C1411-9 PubMed PubMedCentral
  35.  
  36. Satriano J, Matsufuji S, Murakami Y, Lortie MJ, Schwartz D, Kelly CJ, Hayashi S, Blantz RC. Agmatine suppresses proliferation by frameshift induction of antizyme and attenuation of cellular polyamine levels. J Biol Chem. 1998 Jun 19;273(25):15313-6. CrossRef PubMed
  37.  
  38. Mineev VN, Lalaeva TM. Surface architectonics and cytoskeleton of erythrocytes and their modulation by adrenergic agents in patients with bronchial asthma. Ter Arkh. 2004;76(3):12-7. (Russian).
  39.  Bondar TP, Shmarov DA, Kozinets GI Morphometric and biochemical analysis of erythrocytes in smoking patients with late vascular complications of diabetes mellitus. Klin Lab Diagnost., 2003 Aug; (8):37-40. [Russian].
  40.  

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