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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2014; 60(4): 61-69


Tissue hypoxia and related metabolic changes of homeostasis during vibration load in athletes

Hunina LM, Vinnychuk IuD.

    National University of Physical Education and Sport of Ukraine, Kyiv, Ukraine
DOI: https://doi.org/10.15407/fz60.04.061

Abstract

We explored the efficacy and the mechanisms of influence on physical performance of vibratory loads in the “whole body vibration” (WBV) at a frequency of 50 Hz, amplitude of 30 mm and a running time of 30 minutes, which was performed after each standard training sessions in athletes for 3 weeks. WBV vibration causes an occurrence of hypoxia, as evidenced by the end of the study the serum growth athletes content HIF-1 ? with 0.89±0.03 to 1.31±0.01 ng?ml-1, which is substantially higher than in control (1.04±0.02 ng?ml-1). Simultaneously, it is activated such a HIF-1 ?-dependent process as erythropoiesis: the contents of red blood cells increases at the end of the study to 6.04±0.11?1012 l-1 (control only to 5.23±0.12?1012?1-1). Intensification of angiogenic stimuli under the influence of hypoxia is manifested by a significant two times increase in the serum levels of the main angiogenesis factor VEGF (to 62.3±6.8 pg?ml-1, from the control level of 32.7±4.7 pg?ml-1. Vibration loads are accompanied by a lack of oxidative stress, an important ergolytic factor. In contrast, they activate nonenzymatic antioxidant system unit, resulting in an increase in the content of reduced glutathione in the cell membranes. We detected a reduction in endogenous toxicity and improvement of humoral immunity and, as a result, an improvement of physical performance in athletes.

Keywords: physical performance, tissue hypoxia, angiogenesis,erythropoiesis, oxidative stress, cell membranes, humoralimmunity.

References

  1. Mikheev AA. Theory and methods of vibration training in sport (biological and pedagogical rationale dosed of vibrotrening). Monograph. M.: Sov. sport; 2011. [Russian].
  2.  
  3. Issurin VB. Vibrations and their applications in sport: a review. J Sports Med Phys Fitness 2005; 45:324-36. PubMed
  4.  
  5. Vorobieva VV. Vibration model for hypoxic type of cell metabolism evaluated on rabbit cardiomyocytes. Bull Exp Biol Med. 2009; 147(6):768-71. CrossRef PubMed
  6.  
  7. Baykovsky YV. Determining factors of the athlete training at altitude and midlands. M.: TVT Division; 2010. [Russian].
  8.  
  9. Foldes-Papp Z, Domej W, Demel U, Tilz GP. Oxidative stress caused by acute and chronic exposition to altitude. Wien Med Wochenschr 2005;155(7-8):136-42.  CrossRef PubMed
  10.  
  11. Olishevsky CV. Role of the immune system in maintaining health and adaptive capabilities of athletes. Sports Medicine. 2010; (1-2):47-56. [Russian].
  12.  
  13. Semko GA. Structural and functional changes in the external membranes and membranous layers of erythrocytes at hyperepidermopoiesis. Ukr Biochem J. 1998;70:113-8. [Russian].
  14.  
  15. Bankova VV. The method for evaluating the plasma membrane lesions in children with various diseases. Pathol Physiol and Exp Therapy. 1987; (3):78-81. [Russian].
  16. Shvets NI. Age features of changes in glutathione system of rat heart during immobilization stress. Ukr Biochem J. 2008; 80(6):74-8. [Russian].
  17. Kishkun AA Kudinova AC, Ofitova AD, Mishurin RB. Importance of middle molecules in assessing the level of endogenous intoxication. Mil Med J. 1990; (2):41- 44. [Russian].
  18. Lang T, Sesik M. How to describe the statistics in medicine: a guide for authors, editors and reviewers. M.: Practical Medicine; 2011. [Russian].
  19.  
  20. Watts T, Barigou M, Nash G. Comparative rheology of the adhesion of platelets and leukocytes from flowing blood: why are platelets so small? Am J Physiol Heart Circ Physiol. 2013 Jun 1; 304(11):H1483-94. CrossRef PubMed
  21.  
  22. Nikitin VP, Kozyrev SA. The selective effect of a protein kinase C inhibitor on synaptic plasticity in defensive behavior command neurons during development of sensitization in the snail. Neurosci Behav Physiol. 2004; 34(5):423-30. CrossRef  
  23. Panofen F, Piwowarski T, Jeserich G. Small conductance calcium-activated potassium channels of trout CNS: molecular structure, developmental expression, and partial biophysical characterization. Brain Res Mol Brain Res. 2002; 101(1-2):1-11. CrossRef  
  24. Sircar R, Greenswag A, Bilwes A, Gonzalez-Bonet G, Crane B. Structure and activity of the flagellar rotor protein FliY: a member of the CheC phosphatase family. J Biol Chem. 2013; 288(19):13493-502. CrossRef PubMed PubMedCentral
  25.  
  26. Chai Y, Kim D, Park J. The secreted oligomeric form of ?-synuclein affects multiple steps of membrane trafficking. FEBS Lett. 2013;587(5):452-9. CrossRef PubMed
  27.  
  28. Risso A, Turello M, Biffoni F, Antonutto G. Red blood cell senescence and neocytolysis in humans after high altitude acclimatization. Blood Cells Mol Dis. 2007;38(2):83-92. PubMed.
  29.  
  30. Mounier R, Pialoux V, Cayre A, Schmitt L, Richalet J, Robach P, et al. Leukocyte’s Hif-1 expression and traininginduced erythropoietic response in swimmers. Med Sci Sports Exerc. 2006; 38(8):1410-7. PubMed 
  31. Mounier R, Pialoux V, Schmitt L, Richalet JP, Robach P, Coudert J, et al. Effects of acute hypoxia tests on blood markers in high-level endurance athletes. Eur J Appl Physiol. 2009; 106(5):713-20. PubMed" id="ref_href_id" target="blank"">CrossRef
  32. Gunina LM, Lisnyak IA, Burmak VO. Physiological angiogenesis as a factor in adaptation to physical stress athletes with different mechanisms of energy. Bulletin of Sport Science (RF). 2013; (3):34-8. [Russian] 
  33.  
  34. Palazon A, Aragones J, Morales-Kastresana A, de Landazuri M, Melero I. Molecular pathways: hypoxia response in immune cells fighting or promoting cancer. Clin Cancer Res. 2012; 18(5):1207-13. CrossRef PubMed
  35.  
  36. Wagatsuma A. Effect of aging on expression of angiogenesis- related factors in mouse skeletal muscle. Exp Gerontol. 2006; 41(1):49-54. CrossRef PubMed
  37.  
  38. Bartsch P, Saltin B. General introduction to altitude adaptation and mountain sickness (a review) Scand J Med Sci Sports. 2008; Suppl 1:1-10. CrossRef PubMed
  39.  
  40. Goussetis E, Spiropoulos A, Tsironi M. Spartathlon, a 246 kilometer foot race: effects of acute inflammation induced by prolonged exercise on circulating progenitor reparative cells. Blood Cells Mol Dis. 2009; 4(2(3)):294-9. CrossRef PubMed
  41.  
  42. Ariza AC, Deen PM, Robben JH. The succinate receptor as a novel therapeutic target for oxidative and metabolic stress-related conditions. Front Endocrinol (Lausanne) 2012; 3:22-5. CrossRef  
  43. Serebrovskaya TV, Nikolsky IS, Nikolskaya VV, Mallet RT, Ishchuk VA. Intermittent hypoxia mobilizes hematopoietic progenitors and augments cellular and humoral elements of innate immunity in adult men. High Alt Med Biol. 2011 Fall; 12(3):243-52. CrossRef PubMed PubMedCentral
  44.  
  45. McGinnis G, Kliszczewiscz B, Barberio M, Ballmann C, Peters B, Slivka D, et al. Acute hypoxia and exercise-induced blood oxidative stress. Int J Sport Nutr Exerc Metab. 2014 Mar 25; [Epub ahead of print] PubMed.
  46.  
  47. Bell EL, Chandel NS. Mitochondrial oxygen sensing: regulation of hypoxia-inducible factor by mitochondrial generated reactive oxygen species. Essays Biochem. 2007; 43:17-27. CrossRef PubMed
  48.  
  49. Puzhevsky AC, Livshits RI. Kardiodepressivnye agents in the blood of burn patients and evaluation of the effectiveness of their elimination. Anesthesiology and Reanimath. 1990; (1):57-9. [Russian].
  50.  
  51. Anan I, Kiuru-Enari S, Obayashi K, Ranlov P, Ando Y. Investigation of AGE, their receptor and NF-kappaB activation and apoptosis in patients with ATTR and Gelsolin amyloidosis. Histol &Histopaphol. 2010; 25(6):691-9. PubMed
  52.  
  53. Abellan R, Ventura R, Remacha A, Rodriguez F, Pascual J, Segura J. Intermittent hypoxia exposure in a hypobaric chamber and erythropoietin abuse interpretation. J Sports Sci. 2007; 25:1241-50. CrossRef PubMed
  54.  
  55. Wang J-S, Huang Y-H. Effects of exercise intensity on lymphocyte apoptosis induced by oxidative stress in men. Eur J Appl Physiol. 2005; 95:290-1. CrossRef PubMed
  56.  
  57. Michailidis Y, Jamurtas A, Nikolaidis M, Fatouros I, Koutedakis Y, Papassotiriou I, Kouretas D. Sampling time is crucial for measurement of aerobic exerciseinduced oxidative stress. Med Sci Sports Exerc. 2007; 39(7):1107-13. CrossRef PubMed
  58.  
  59. Nikolaidis M, Jamurtas A, Paschalis V, Fatouros I, Koutedakis Y, Kouretas D. The effect of muscle-damaging exercise on blood and skeletal muscle oxidative stress: magnitude and time-course considerations. Sports Med. 2008; 38(7):579-606. CrossRef PubMed
  60.  

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