Acute L-glutamine deprivation affects the expression of TP53-related protein genes in U87 glioma cells.
Minchenko DO1,2, Danilovskyi SV1, Kryvdiuk IV1, Hlushchak NA1, Kovalevska OV1, Karbovskyi LL1, Minchenko OH1
- Department of Molecular Biology, Palladin Institute of Biochemistry NationalAcademy of Sciences of Ukraine, Kyiv, Ukraine;
- Departments of Pediatrics, Bogomolets National Medical University, 13Shevchenka Bvld., 01601, Kyiv, Ukraine
DOI: https://doi.org/10.15407/fz60.04.011
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Abstract
Ми дослідили ефект гострого дефіциту L-глутаміну на
експресію таких генів, залежних від протеїну пухлин р53
(TP53), як RYBP, TOPORS, TP53BP1, TP53TG1, SESN1,
NME6, та ZMAT3 у клітинах гліоми з виключеною актив-
ністю ERN1. Показано, що блокада функції гена ERN1
у клітинах гліоми лінії U87 посилює експресію генів
RYBP та SESN1, при цьому інтенсивність експресії генів
TP53BP1, TP53TG1, TOPORS, NME6 та ZMAT3 знижу-
ється. Більше того, рівень експресії генів RYBP, SESN1,
TP53BP1 та ZMAT3 збільшується у контрольних клітинах
гліоми за умов дефіциту L-глутаміну у середовищі, але
виключення функції ензиму ERN1 істотно посилює цей
ефект на експресію всіх цих генів. Водночас виключення
функції ензиму ERN1 знімає залежність експресії генів
TP53TG1 та TOPORS від дефіциту L-глутаміну. Результати
цієї роботи вказують на залежність експресії більшості
асоційованих з TP53 генів від умов гострого дефіциту глу-
таміну у середовищі, як і від ERN1, основної сигнальної
системи стресу ендоплазматичного ретикулума.
Keywords:
дефіцит глутаміну, стрес ендоплазматич-ного ретикулума, RYBP, TP53BP1, TP53TG1, TOPORS,SESN1, ZMAT3, NME6, експресія генів.
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