Propargylglycine restores endothelium-dependent relaxation of aortic smooth muscles in old rats
Drachuk KO, Kotsiuruba AV, Baziliuk OV, Stepanenko LH, Sahach VF.
O.O. Bogomoletz Institute of Physiology, National Academy of Sciences of Ukraine, Kyiv, Ukraine
DOI: https://doi.org/10.15407/fz60.04.003
Abstract
In the study we investigated the effect of blockade сystathionine-
? -lyase (CSE), an enzyme of hydrogen sulfide (H2S) (de
novo) synthesis on the endothelium-dependent relaxation of
aortic smooth muscle (SM) in old rats. It has been shown that
an inhibition of CSE by propargylglycine (PAG) results in
restoration of a decreased ACh-induced relaxation of aorta in
old rats. This effect of PAG was removed by blocking nitric
oxide (NO) synthesis in the endothelial cells. Age-related
changes in the levels of H2S, NO2
- and enzyme activity of
the constitutive synthesis of NO (cNOS) in the heart, were
determined. It has been shown that PAG introduction elevates
a decreased levels of H2S, NO2
-
and stimulates the suppressed
activity of cNOS in old rats. These results suggest that PAG
activates alternative ways of H2S synthesis and stimulates the
constitutive synthesis of NO. These actions of PAG restore
endothelial function in old rats.
Keywords:
propargylglycine, hydrogen sulfide, cystathionine-г-lyase, acetylcholine, nitrogen oxide, endothelium, smoothmuscle, aorta, aging, oxidative stress.
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