Українська English

ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2014; 60(3): 80-88

The activity of hyaluronate-binding proteins in the brain and pancreas during experimental chronic pancreatitis in rats

V.A. Makarchuk1, O.V. Zeleniuk2, H.O. Ushakova2

  1. SI “Institute of Gastroenterology of National Academy of Medical Sciences of Ukraine”, Dnipropetrovsk, Ukraine
  2. Oles Gonchar Dnipropetrovsk National University, Dnipropetrovsk, Ukraine
DOI: https://doi.org/10.15407/fz60.03.080


Abstract

Cell contact with hyaluronic acid as a component of the extracellular matrix plays an important role in the development of many physiological and pathological processes. In experiments on laboratory rats, the model of chronic pancreatitis was reproduced. Prolonged occlusion of the pancreatic duct caused the pancreatic acinar cells atrophy and pancreatic fibrosis. It was found that the development of chronic pancreatitis accompanied by an increase of concentrations of hyaluronic acid in plasma as a marker of fibrosis of the pancreas. The redistribution of hyaluronate-binding activity of the receptor in the pancreas and the brain: cerebellum, hippocampus and thalamus were observed. The most pronounced redistribution of degree of affinity to hyaluronate was observed in the mem- В.А. Макарчук, О.В. Зеленюк, Г.О. Ушакова 88 ISSN 0201-8489 Фізіол. журн., 2014, Т. 60, № 3 brane fraction of proteins: in the cerebellum and thalamus the sensitivity to this glycosaminoglycan was decreased, while in the hippocampus the sensitivity was increased, which may serve as a signal for some adaptive processes in these parts of the brain under the influence of factors that accompany the development of chronic pancreatitis.

Keywords: chronic pancreatitis, pancreas, cerebellum, hippocampus,thalamus, hyaluronic acid, hyaluronate-bindingactivity of the protein.

Full text: (PDF, in Ukrainian)

References

  1. Stepanov YuM, Zaichenko NG. Chronic pancreatitis:biliary mechanism, factors and course. Zaporozhye Med J. 2012; 70(1): 46-50.
    2.  
  2. Frokj?r JB, Olesen SS, Gram M, Yavarian Y, Bouwense SA, Wilder-Smith OH,Drewes AM. Altered brain microstructure assessed by diffusion tensor imaging in patients with chronic pancreatitis. Gut. 2011; 60(11): 1554-1562. CrossRef PubMed
    3.  
  3. Frokj?r JB, Bouwense SA, Olesen SS, Lundager FH, Eskildsen SF, van Goor H, Wilder-Smith OH, Drewes AM. Reduced Cortical Thickness of Brain Areas Involved in Pain Processing in Patients With Chronic Pancreatitis. Clin Gastroenterol and Hepatol. 2012; 10(4): 434-438. CrossRef PubMed
    4.  
  4. Cargill R, Kohama SG, Struve J, Su W, Banine F, Witkowski E, Back SA, Sherman LS. Astrocytes in aged nonhuman primate brain gray matter synthesize excess hyaluronan. Neurobiol Aging. 2012; 33(4): 830.e13–830.e24. CrossRef PubMed PubMedCentral
    5.  
  5. Choi K.Y., Saravanakumarc G., Parkc J.H., Parka K. Hyaluronic acid-based nanocarriers for intracellular targeting: Interfacial interactions with proteins in cancer. Colloids and Surfaces B: Biointerfaces. 2012; 99: 82-94. CrossRef PubMed PubMedCentral
    6.  
  6. Weinstein-Oppenheimer CR, Aceituno AR, Brown DI, Acevedo C, Ceriani R, Fuentes MA, Albornoz F, Henriquez-Roldan CF, Morales P, Maclean C, Tapia SM, Young ME.The effect of an autologous cellular gel-matrix integrated implant system on wound healing. J Translational Med. 2010; 8:59. CrossRef PubMed PubMedCentral
    7.  
  7. Frischknecht R, Gundelfinger ED. The Brain's Extracellular Matrix and Its Role in Synaptic Plasticity. Advances in Exp Med and Biol. 2012; 970: 153-171. CrossRef PubMed
    8.  
  8. Liang J, Jiang D, Jung Y, Xie T, Ingram J, Church T, Degan S, Leonard M, Kraft M, Noble PW. Role of hyaluronan and hyaluronan-binding proteins in human asthma. J. Allergy Clin Immunol. 2011; 128(2): 403-411. CrossRef PubMed PubMedCentral
    9.  
  9. Sirenko OYu. Pancreatic stellate cells as a morphological basis for the development of pancreatic fibrosis. Morphology. 2010; 4(1): 5-12.
    10.  
  10. Ding Z, Liu J, Lin R, Hou XH. Experimental pancreatitis results in increased blood-brain barrier permeability in rats: A potential role of MCP-1. J Digestive Diseases. 2012; 13(3): 179-185. CrossRef PubMed
    11.  
  11. Hornik A, Porcel F, Agha C, Flaster M, Vidal SM, Schneck MJ, Lee J, Biller J.Central and Extrapontine Myelinolysis Affecting the Brain and Spinal Cord. An Unusual Presentation of Pancreatic Encephalopathy. Frontiers in Neurology. 2012; 3: 135. CrossRef PubMed PubMedCentral
    12.  
  12. Page BJ, Toit DF, Muller C, Mattysen J, Lyners R. An Immunocytochemical Profile of the Endocrine Pancreas Using an Occlusive Duct Ligation Model. J Pancreas. 2000; 4(1): 191-203.
    13.  
  13. Sapoznikov A.G., Dorosevich A.E. Histological and microscopical technique: Handbook. – Smolensk: "SAU", 2000: 477 p.
    14.  
  14. Bradford M. Rapid and sensitive methods for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem.1985; 72(1-2): 248-254. CrossRef  
  15. Dolzhenko MI, Lepekhin EA, Berezin VA. A novel methods for evalution of carbohydrate-binding activity: enzyme-linked carbohydrate-binding assay (ELCBA). Biochem Mol Biol Intern. 1994; 34(2): 261-271. PubMed
    16.  
  16. Gold EW. The quantitative spectrophotometric estimation of total sulfated glycosaminoglican levels. Biochem. et Biophys Acta. 1981; 673(4): 408-415. CrossRef  
  17. The visible statistics in medicine. Edited by A. Petri, KM Syebin: Heotar-Med, 2003: 144 p.
    18.  
  18. Adrych K., Smoczynski M., Stojek M., Sledzinsk T., Korczynska J., Goyke E., Swierczynski J. Coordinated increase in serum platelet-derived growth factor-BB and transforming growth factor-v1 in patients with chronic pancreatitis. Pancreatology. 2011; 11(4): 434-440. CrossRef PubMed
    19.  
  19. Jackson DG. Immunological functions of hyaluronan and its receptors in the lymphatics. Immunol Rev. 2009; 230(1): 216-231. CrossRef PubMed
    20.  
  20. Jiang D, Liang J, Noble PW. Hyaluronan as an immune regulator in human diseases. Physiol Rev. 2011; 91(1): 221-264. CrossRef PubMed PubMedCentral
    21.  
  21. Johnsson C, Hallgren R, Tufveson G. Role of hyaluronan in acute pancreatitis. Surgery. 2000; 127(6): 650-658. CrossRef PubMed
    22.  
  22. Lu XL, Song YH, Fu YB, Si JM, Qian KD. Ascorbic acid alleviates pancreatic damage induced by dibutyltin dichloride in rats. Yonsei Med. J. 2007; 48 (6): 1028-1034. CrossRef PubMed PubMedCentral
    23.  
  23. Wang CH, Gao ZQ, Ye B, Cai JT, Xie CG, Qian KD, Du Q. Effect of emodin on pancreatic fibrosis in rats. World J Gastroenterol. 2007; 13(3): 378-382. CrossRef PubMed PubMedCentral
    24.  
  24. Sadowitz B, Seymour K, Gahtan V, Maier KG. The role of hyaluronic acid in atherosclerosis and intimal hyperplasia. J Surg Res. 2012; 173(2): 63-72. CrossRef PubMed
    25.  
  25. Sleeman J, Rudy W, Hofmann M, Moll J, Herrlich P, Ponta H. Regulated clustering of variant CD44 proteins increases their hyaluronate binding capacity. J Cell Biol.1996; 135(4): 1139-1150. CrossRef PubMed
    26.  

© National Academy of Sciences of Ukraine, Bogomoletz Institute of Physiology, 2014-2024.