The effect of enzymatic treatment using proteases on properties of persistent sodium current in CA1 pyramidal neurons of rat hippocampus
Lun'ko OO, Isaiev DS, Maxymiuk OP, Kryshtal' OO, Isaieva OV
O.O. Bogomoletz Institute of Physiology, National Academy of Sciences of Ukraine, Kyiv, Ukraine
DOI: https://doi.org/10.15407/fz60.03.075
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Abstract
We investigated the effect of proteases, widely used for neuron
isolation in electrophysiological studies, on the amplitude
and kinetic characteristics of persistent sodium current (INaP)
in hippocampal CA1 pyramidal neurons. Properties of INaP
were studied on neurons isolated by mechanical treatment
(control group) and by mechanical and enzymatic treatment
using pronase E (from Streptomyces griseus) or protease type
XXIII (from Aspergillus oryzae). We show that in neurons
isolated with pronase E kinetic of activation and density of
INaP was unaltered. Enzymatic treatment with protease type
XXIII did not alter INaP activation but result in significant
decrease in INaP density. Our data indicates that enzymatic
treatment using pronase E for neuron isolation is preferable
for investigation of INaP.
Keywords:
persistent sodium current, enzymatic treatment,protease, hippocampus.
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