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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2014; 60(3): 38-45


Changes in gastric function in rats after intragastric introduction of corvitin at high doses

Vovkun TV, Ianchuk PI, Shtanova LIa, Vesel'skyĭ SP, Baranovs'kyĭ VA.

    Taras Shevchenko National University, Kyiv, Ukraine
DOI: https://doi.org/10.15407/fz60.03.038

Abstract

Intragastric administration of corvitin at doses of 10, 20 and 40 mg/kg dose-dependently increased the volume of gastric juice and the total production of hydrochloric acid (HA). Amplification of hexosamines and cysteine production was observed only when the study drug was administered at a dose of 10 mg/kg. When corvitin was used at 20 and 40 mg/kg, these parameters were at the level of control values. Protein production increased in response to 10 and 20 mg/kg corvitin, but fell below the control values after administration of 40 mg/ kg of the drug. The level of blood flow in the gastric mucosa increased following administration of 10 mg/kg corvitin, was not different from the baseline after 20 mg/kg of the drug and significantly decreased in response to 40 mg/kg of flavonoid. Our results indicate that a single intragastric application of corvitin at dose of 10 mg/kg activates gastric defense mechanisms. At 20 and 40 mg/kg, corvitin does not affect them but gradually reduces blood flow in gastric mucosa, causes a disturbance of protein synthesis and hypersecretion of HA into the cavity of the stomach, which can lead to disruption of the digestive process and the integrity of gastric mucosa.

Keywords: stomach, gastric mucosa, corvitin, blood flow,gastric secretion, hydrochloric acid, protein, hexosamines,cysteine

References

  1. Middleton E, Kandaswami C, Theoharides TC. The effects of plant flavonoids of mammalian cells: implications for inflammation, heart disease, and cancer. Pharmacol Rev 2000;52:673-751. PubMed
  2.  
  3. Gutzeit HO, Henker Y, Kind B, Franz A. Specific interactions of quercetin and other flavonoids with target proteins are revealed by elicited fluorescence. Biochem Biophys Res Commun 2004;318(2):490-495. CrossRef PubMed
  4.  
  5. Maroziene A, Nemeikaite-Ceniene A, Vidziunaite R, Cenas N. Correlation between mammalian cell cytotoxicity of flavonoids and the redox potential of phenoxyl radical/phenol couple. Acta Biochim Pol 2012;59(2):299-305. PubMed
  6.  
  7. Ranavat P, Pathak CM, Khanduja KL. A new perspective on the quercetin paradox in male reproductive dysfunction. Phytother Res 2013;27(6):802-10. CrossRef PubMed
  8.  
  9. Kesarwani K, Gupta R, Mukerjee A. Bioavailability enhancers of herbal origin: an overview. Asian Pac J Trop Biomed. 2013;4:253-266. CrossRef  
  10. Mokhort NA, Shalamay AS, Frantsuzova SB. Study of pharmacotherapeutic properties dosage forms of quercetin. In: Moibenko AA. Bioflavonoids as organoprotectors (quercetin,corvitin, quertin). Kiev: Naukova Dumka; 2012. p.74-117.
  11.  
  12. Vovkun TV, Yanchuk PI, Shtanova LY, Veselskiy SP, Baranowskiy VA. Effect of Corvitin on secretory processes and blood flow in the gastric mucosa of rats.Physiol Zh. 2013;59(1):40-47. CrossRef  
  13. Shay H, Komarov S, Fels SS, Meranze D, Gruenshtein M, Siplet H. A simple method for the uniform production of gastric ulceration in the rat. Gastroenterol. 1945;5:43-61.
  14.  
  15. Kochetkov GA. A practical guide to enzymology. Moscow: Higher School; 1980. 272 p.
  16.  
  17. Korobeinikova EM, Mescherynova TV. Determination of free amino acids in the serum and urine of healthy children. Lab Work 1981;4:221-224.
  18.  
  19. Yanchuk PI, Palatniy TP, Rusinchuk YI. Modified electrode for registration of local blood flow in the gastric mucosa by hydrogen clearance. Rus Physiol J.2005;91(9):1108-1110.
  20.  
  21. Cross C, Halliwell B, Allen A. Antioxidant protection: a function of tracheobronchial and gastrointestinal mucus. Lancet. 1984;1:1328 -1330. CrossRef  
  22. Seno K, Joh T, Yokoyama Y, Itoh M. Role of mucus in gastric mucosal injury induced by local ischemia/reperfusion. J Lab Clin Med 1995;126(3):287-293. PubMed
  23.  
  24. Sairam K, Rao ChV, Babu DM, Kumar V, Agrawal VK, Goel RK. Antiulcerogenic activity of methanolicextract of Emblica officinalis. J Ethnopharm 2002;82(1):1-9. CrossRef  
  25. Santhosh S, Anandan R, Sini TK, Mathew PT. Protective effect of glucosamine against ibuprofen-induced peptic ulcer in rats. J Gastroenter Hepatol. 2007;22(6):949-53. CrossRef PubMed
  26.  
  27. Komorowska M, Szafran H, Szafran Z, Popiela T. Free amino acids in basal and vagally stimulated gastric secretion. Acta Physiol Pol 1989;40(5):496-503. PubMed
  28.  
  29. Szabo S, Nagy L, Plebani M. Glutathione, protein sulfhydryls and cysteine proteases in gastric mucosal injury and protection. Clin Chim Acta 1992;206(1):95-105. CrossRef  
  30. Laine L, Takeuchi K, Tarnawsky A. Gastric mucosal defence and cytoprotection: bench to bedside. Gastroenterol 2008;135(1):41–60. CrossRef PubMed
  31.  
  32. Robaszkiewicz A, Balcerczvk A, Bartosz G. Antioxidative and prooxidative effects of quercetin on A549 cells. Cell Biol Int. 2007;31(10):1245-50. CrossRef PubMed
  33.  
  34. Dunnick JK, Hailey JR. Toxicity and carcinogenicity studies of quercetin, a natural component of foods. Fundam Appl Toxicol. 1992;19(3):423-31. CrossRef  
  35. Banerjee N, Van der Vliet A, Ziboh VA. Downregulation of COX-2 and iNOS by amentoflavone and quercetin in A549 human lung adenocarcinoma cell line. Prostagland Leukotr Essent Fatty Acids. 202;66 Suppl 5:485-92.
  36.  
  37. Araki H, Ukawa H, Sugawa Y, Yagi K, Suzuki K, Takeuchi K. The roles of prostaglandin E receptor subtypes in the cytoprotective action of prostaglandin E2 in rat stomach. Aliment Pharm and Ther. 2000;14 Suppl 1:116-124. CrossRef PubMed
  38.  
  39. Shiesi M, Shwaller R. Inhibition of constitutive endothelial NO-synthase activity by tannin and quercetin. Biochem Pharm. 1995;49(4):495-501. CrossRef  
  40. Whittle BJ. Neuronal and endothelium-derived mediators in the modulationof the gastric microcirculation^integrity in the balance. Br J Pharmacol. 1993;110:3-17. CrossRef PubMed PubMedCentral
  41.  
  42. Shtanova LY. Effects of exogenous and endogenous nitric oxide on gastric acid secretion in rats. Physics of the live. 2008;16(1):128-133.
  43.  
  44. Nishida K, Ohta Y, Ishiguro I. Relationship between constitutive nitric oxide synthase activity and mucus level in the gastric mucosa of rats with stress. Pharmacol Res.1998;38(5):393-400 CrossRef PubMed

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