Українська Русский English

ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2014; 60(2): 82-87


The impact of male phytoestrogenization on the somato-sexual development and fertility of the offsprings in rats

Seliukova NIu, Karpenko NO, Korenieva IeM, Somova OV, Smolienko NP, Pocherniaieva SS, Brechka NM

    SI « V Danilevsky Institute of Endocrine Pathology Problems of the NAMS of Ukraine», Kharkiv, Ukraine
DOI: https://doi.org/10.15407/fz60.02.082

Abstract

The effect of phytoestrogen-rich diet administered to male rats in a dose 20 mg / kg of body weight for 30 days with the mixture of phytoestrogens on reproductive function of male offspring and the effect of additional phytoestrogenization during milk feeding have been investigated. It was shown that male phytoestrogenization leads to feminization of the offsprings. Specifically, at birth, the ano-genital distance in males was less than that measured in control rats: (2,7 ± 0,1) vs (3,1 ± 0,0) mm (Р <0,05). This and additional phytoestrogenization of newborn rats during milk feeding inhibit their somatic and sexual development, weakening sexual activity in adults and reducing fertility at the expense of dectreased fertilizing capacity of sperm. A possible mechanism for the observed effects may be a reduction of relative androgenization due to increased of estradiol concentration by 3-4 times. Analysis of the data revealed a significant impact of phytoestrogens in father’s diet on reproductive function of their offsprings under conditions of additional phytoestrogenization after birth

Keywords: phytoestrogens, descedants, sexual behavior,spermatogenesis.

References

  1. Koreneva EM, Karpenko NA. Phytoestrogens. Influence on the reproductive system. Problems Endocrine Pathology 2007; 3:87-95.
  2.  
  3. Wigle D, Arbuckle T, Tuner M. Epidemiologic evidence of relationships between reproductive and child health outcomes and environmental chemical contaminants. J. Toxicol. Environ. Health. B. Crit. Rev 2008; 11(5-6):373-517. CrossRef PubMed
  4.  
  5. Shepelskaya NR, Prodanchuk MG. Soy phytoestrogens and their anti-androgenic action. Problems harch 2010; 3-4:26-31.
  6.  
  7. Wisniewski AB, Klein SL, Lakshmanan Y, Gearhart JP. Expisure to Genistein during Gestation and lactation demasculinized the reproductive system in rats. J Urol 2003; 169(4):1582-6. CrossRef PubMed
  8.  
  9. Gladkova A, ZolotukhinaV, Klimova A. Features consequences of hyperandrogenism of a parent for a offspring. Endocrinology 2001; 6:6.
  10.  
  11. Chistyakova EE. Features of reproductive disorders of male offspring received from male rats that have had stress. Thesis fundamental and Clinical endocrinology: problems, achievements and perspectives (7th Danilevsky reading), Kharkov, 21-22 February. p. 144-5
  12.  
  13. Karpenko NA, Chub N, Bryzgalova G. Effect of longterm low-dosage of irradiation of germ cells in male gametogenesis postmeyotychniy phase of spermatogenesis in their offspring. UJ of Radiology 2000; 8:73-76.
  14.  
  15. Hu WY, Wu SH, Wang LL. A toxicological and epidemiological study of reproductive functions of male workers exposed to lead. J. Hyg. Epidemiol. Microbiol.Immunol 1992; 36(1):25-30. PubMed
  16.  
  17. General ethical principles of animal experimentation. Endocrinology 2003; 8(1):142-5.
  18.  
  19. Gladkova AI Yaremenko FG, Nikishina LE, Kravchenko SV. Study of the composition of soy product Genistein Soy Complex using chromatographic methods. Thesis conference Kharkiv, 3-4 March. 2011 p. 29-30.
  20.  
  21. Byshovets TF. Experimental study embryotoxical action of medicines: methodological recommendations. Preclinical studies of medicines; 2001.
  22.  
  23. Vorobyova OA, Leontyeva OA, Korsak VS. Effect sperm morphology on of fertilization frequency and early embryonic development disorders. Cytology 1996;38(2):1248-54.
  24.  
  25. Rehfeldt C, Kalbe C, Nurnberg G, Mau M. Dosedependent effects of genistein and daidzein on protein metabolism in porcine myotube cultures. J Agric Food Chem 2009; 57(3):852-7. CrossRef PubMed
  26.  
  27. Simmen FA, Mercado CP, Zavacki AM, Huang SA, Greenway AD, Kang P, Bowman MT, Prior RL. Soy protein diet alters expression of hepatic genes regulating fatty acid and thyroid hormone metabolism in the male rat. J Nutr Biochem 2010; 21(11):1106-13. CrossRef PubMed
  28.  
  29. Smolyenko NP, Gladkova AI Karpenko N. Deleted consequences of phytoestrogens in different age periods for a sexual behavior of male rats. Thesis scientific and practical Conference «Achievements and Prospects of Experimental and Clinical Endocrinology» (9th Danilevsky reading), Kharkiv, 2-3 March. 2010 p.122-3.
  30.  
  31. McClein RM, Wolz E, Davidovich A. Reproductive safety studies with genistein in rats. Food. Chem. Toxicol 2007; 45(8):1319-32. CrossRef PubMed

© National Academy of Sciences of Ukraine, Bogomoletz Institute of Physiology, 2014-2019.