Українська English

ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2014; 60(2): 70-74

Effects of natural antioxidants on the content of lipids, proteins and carbohydrates in rat's gastric mucosal cells under experimental ulceration

Koval'ova VA, Shevchenko AIe, Ostapchenko LI.

    Taras Shevchenko National University, ESC “Institute ofBiology”, Kyiv, Ukraine
DOI: https://doi.org/10.15407/fz60.02.070


Abstract

Effects of natural antioxidants on the contents of lipids, proteins and carbohydrates in rat’s gastric mucosal cells under experimental ethanolinduced ulceration have been studied. A significant decrease in the contents of neutral and phospholipids was found under experimental ulceration and their increase was detected under addition of squalene and ammivit. Under ulceration we found a significant decrease of protein fractions with molecular weight within the range of 11-138 kDa, galactose and fucose contents. Following addition of squalene and ammivit, a normalization of these parameters was observed.

Keywords: ethanol ulcer, gastric mucosa, ammivit, squalene,galactose, fucose.

Full text: (PDF, in Ukrainian)

References

  1. Vasylenko V.Kh., Hrebenev A.L., Sheptulyn A.A. [Ulcer desease]. M.: Medicine;1987. 285 p. Russian.
  2. Smyrnov Iu.V., Oslopov V.N., Bylych Y.L. [Epidemiological aspects of the combination of hypertension and ulcer desease]. Ter. arkh. 1990;2:48-50. Russian.
    3.  
  3. Saenko V.F., Homoliako Y.V., Buryi A.N. [Features of associated with Helicobacter pylori infection stomach and duodenum diseases’ diagnosis and treatment in the surgical clinic]. Klinichna khirurhiia. 2001;6:14-18. Ukrainian.
    4.   
  4. Shetty R., Kumar K.V., Naidu M.U.R., Ratnakar K.S. Effect of Gingko biloba extract on ethanol-induced gastric mucosal lesions in rats. Indian J. of Pharmacology. 2000;32 (6):313-317.
    5.  
  5. Marhuenda U.E., Martin M.J. and Alarcon-de-Lastra C. Anti-ulcerogenic activity of aescine in different experimental models. Phytother. 1993;22 (7) :13-16. CrossRef  
  6. Hemandez R., Munoz C., Montel Ruiz and Vazquez O. Martinez. Gastric mucosal cell proliferation in ethanolinduced chronic mucosal injury is related to oxidative stress and lipid peroxidation in rats. Lab. Invest. 2000;80 (1) :1161-1169.
    7.  
  7. Jainu M. and Devi C.S. Antiulcerogenic and ulcer healing effects of Solanum nigrum (L.) on experimental ulcer models. Possible mechanism for the inhibition of acid formation. J. Ethnopharmacol. 2006;104 (10) :156-163. CrossRef PubMed
    8.  
  8. Zapadniuk Y.P., Zapadniuk V.Y., Zakharyia E.A., Zapadniuk B.V. [Laboratory animals. Breeding, keeping, using in experiment]. K.: Vyshcha shkola;1983. 383 p. Russian.
    9.   
  9. Academician, prof. RAMN Yvashkyn V.T., prof. Rapoport S.Y., editors. [Gastroenterology directory of practical doctor]. M.: Sov. sport; 1999. 432 p. Russian
    10.  
  10. Levin, G.G., Bulygin Th.V., Vishnyakov G.N. Coherent Oscillations Of The Molecular State Of Protein In Live Cells. Tsitologiya. 2005;47: 348-356.
    11.  
  11. Laemmli U.K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970; 227: 680-685. CrossRef  
  12. Kates M. Techniques of lipidology, isolation, analysis and identification of lipids. Laboratory Techniques in Biochemistry and Molecular Biology. Amsterdam, NY: North-Holland Pub. Co.American Elsevier;1972.p. 269-610.
    13.  
  13. Dische Z., Shettles L.B. A specific color reaction of methylpentoses and a spectrophotometric micromethod for the determination. J. of Biol. Chemistry. 1948; 175 (2):595-603. PubMed
    14.  
  14. Uholev A. M., Yezuytova N. N. [Determination of invertase's and other disaccharidases' activity. Investigation of the digestive tract in humans]. L.: Nauka;1969. p.192-196.
    15.  
  15. Handel D.U., Kittlak W. Vergleichende Untersuchung zur Metodik der Bestimmung des eiwei gedundenen suckers. Z. Med. Labor. Techn. 1963; 4:163-169.
    16.  
  16. Lowry O.H., Rosebrough N.J., Farr A.L., Randal R.I. Protein measurement with Folin phenol reagent. J. Biol. Chem. 1951;193(1). : 265-275. PubMed
    17.  
  17. Wallace J., Grander D. FASEB J.1996; 10: 731-740. CrossRef PubMed
    18.  
  18. Elkins R. Miracle Sugars. Woodland Publishing; 2003.
    19.  
  19. Kolset S., Prydz K., Pejler G. Biochem. J. 2004; 379: 217-227. CrossRef PubMed PubMedCentral
    20.  
  20. Ogata M., Araki K., Ogata T. Histol. Histopathol. 1998; 13: 347-348. PubMed
    21.  

© National Academy of Sciences of Ukraine, Bogomoletz Institute of Physiology, 2014-2025.