Українська English

ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2014; 60(2): 63-69

The effect of probiotic therapy on development of experimental obesity in rats caused by monosodium glutamate

Savcheniuk OA1, Virchenko OV1, Falalieieva TM1, Beregova TV1, Babenko LP1, Lazarenko LM2, Spivak MIa2

  1. Taras Shevchenko National University, Kyiv, Ukraine
  2. Zabolotnyi Institute of microbiology and virusology NAS ofUkraine, Kyiv, Ukraine


The effect of a mixture of probiotic strains (2:1:1 Lactobacillus casei IMVB-7280, Bifidobacterium animalis VKL, Bifidobacterium animalis VKB) on the development of experimental obesity in rats induced by neonatal administration of monosodium glutamate has been studied. It was shown that in rats of 4 months age, the injection of monosodium glutamate (4 mg/g) at 2, 4, 6, 8, 10 days after birth elicited abdominal obesity and metabolic syndrome. An intermittent administration of a probiotic mixture to rats treated with monosodium prevented the development of obesity. In the group of rats treated with probiotics, anthropometric parameters (weight and body length, Lee index, body mass index) did not differ from the level of intact rats. Visceral fat mass was decreased by probiotics by 38,5 % (P<0,05) compared to rats treated with water. Probiotics improved lipid metabolism: reduced the level of VLDL by 32,2 % (P<0,05), the level of LDL by 30,6 % (P<0,05), increased HDL by 25,7 % (P<0,05) compared to obese control rats. Probiotic strains restored the secretion of adipocytes hormones (leptin and adiponectin) to the normal level of intact animals. The results show the effectiveness of probiotics for the prevention of obesity.

Keywords: obesity, MSG, probiotics


  1. Ginsburg M. [Obesity and metabolic syndrome. Impact on health, prevention and treatment]. Samara: Parus; 2000.
  3. Yang W, Kelly T, He J. Genetic epidemiology of obesity. Epidemiol Rev. 2007;29:49-61. CrossRef PubMed
  5. Angelakis E, Bastelica D, Ben Amara A, El Filali A, Dutour A, Mege JL et al. An evaluation of the effects of Lactobacillus ingluviei on body weight, the intestinal microbiome and metabolism in mice. Microb Pathog. 2011Jan;52(1):61-8. CrossRef PubMed
  7. Kim SW, Park KY, Kim B, Kim E, Hyun CK. Lactobacillus rhamnosus GG improves insulin sensitivity and reduces adiposity in high-fat diet-fed mice through enhancement of adiponectin production. Biochem Biophys Res Commun. 2013 Feb 8;431(2):258-63. CrossRef PubMed
  9. Luoto R, Laitinen K, Nermes M, Isolauri E. Impact of maternal probiotic-supplemented dietary counseling during pregnancy on colostrum adiponectin concentration: a prospective, randomized, placebo-controlled study. EarlyHum Dev. Jun;88(6):339-44.
  11. Backhed F, Ding H, Wang T, Hooper LV, Koh GY, Nagy A, et al. The gut microbiota as an environmental factor that regulates fat storage. Proc Natl Acad Sci U S A. 2004Nov 2;101(44):15718-23. CrossRef PubMed PubMedCentral
  13. Hildebrandt MA, Hoffmann C, Sherrill-Mix SA, Keilbaugh SA, Hamady M, Chen YY, et al. High-fat diet determines the composition of the murine gut microbiomeindependently of obesity. Gastroenterology. 2009Nov;137(5):1716-24 e1-2.
  15. Turnbaugh PJ, Backhed F, Fulton L, Gordon JI. Dietinduced obesity is linked to marked but reversible alterations in the mouse distal gut microbiome. Cell Host Microbe. 2008 Apr 17;3(4):213-23. CrossRef PubMed PubMedCentral
  17. Cani PD, Neyrinck AM, Fava F, Knauf C, Burcelin RG, Tuohy KM, et al. Selective increases of bifidobacteria in gut microflora improve high-fat-diet-induced diabetes in mice through a mechanism associated with endotoxaemia.Diabetologia. 2007 Nov;50(11):2374-83. CrossRef PubMed
  19. Fak F, Backhed F. Lactobacillus reuteri prevents diet-induced obesity, but not atherosclerosis, in a strain dependent fashion in Apoe-/- mice. PLoS One. 2012;7(10):e46837. CrossRef PubMed PubMedCentral
  21. Kadooka Y, Sato M, Imaizumi K, Ogawa A, Ikuyama K, Akai Y, et al. Regulation of abdominal adiposity by probiotics (Lactobacillus gasseri SBT2055) in adults with obese tendencies in a randomized controlled trial. Eur J Clin Nutr. 2010 Jun;64(6):636-43. CrossRef PubMed
  23. Luoto R, Kalliomaki M, Laitinen K, Isolauri E. The impact of perinatal probiotic intervention on the development of overweight and obesity: follow-up study from birth to 10 years. Int J Obes (Lond). 2010 Oct;34(10):1531-7. CrossRef PubMed
  25. Leshchenko IV, Shevchuk VH, Falalieieva TM, Beregova TV. [The influence of long-term monosodium glutamate feeding on the structure of rats pancreas]. Fiziol Zh. 2012;58(2):59-65. PubMed
  27. Oida K, Nakai T, Hayashi T, Miyabo S, Takeda R. Plasma lipoproteins of monosodium glutamate-induced obese rats. Int J Obes. 1984;8(5):385-91. PubMed
  29. Hara K, Boutin P, Mori Y, Tobe K, Dina C, Yasuda K, et al. Genetic variation in the gene encoding adiponectin is associated with an increased risk of type 2 diabetes in the Japanese population. Diabetes. 2002 Feb;51(2):536-40. CrossRef PubMed
  31. Hotta K, Funahashi T, Bodkin NL, Ortmeyer HK, Arita Y, Hansen BC, et al. Circulating concentrations of the adipocyte protein adiponectin are decreased in parallel with reduced insulin sensitivity during the progression to type 2 diabetes in rhesus monkeys. Diabetes. 2001 May;50(5):1126-33. CrossRef PubMed
  33. Combs TP, Pajvani UB, Berg AH, Lin Y, Jelicks LA, Laplante M, et al. A transgenic mouse with a deletion in the collagenous domain of adiponectin displays elevated circulating adiponectin and improved insulin sensitivity. Endocrinology. 2004 Jan;145(1):367-83. CrossRef PubMed
  35. Berg AH, Combs TP, Scherer PE. ACRP30/adiponectin: an adipokine regulating glucose and lipid metabolism. Trends Endocrinol Metab. 2002 Mar;13(2):84-9. CrossRef  
  36. Maeda N, Takahashi M, Funahashi T, Kihara S, Nishizawa H, Kishida K, et al. PPARgamma ligands increase expression and plasma concentrations of adiponectin, an adipose-derived protein. Diabetes. 2001 Sep;50(9):2094-9. CrossRef PubMed
  38. Nawrocki AR, Rajala MW, Tomas E, Pajvani UB, Saha AK, Trumbauer ME, et al. Mice lacking adiponectin show decreased hepatic insulin sensitivity and reduced responsiveness to peroxisome proliferator-activated receptor gamma agonists. J Biol Chem. 2006 Feb 3;281(5):2654-60. CrossRef PubMed
  40. Dedov I, Balabolkin M, Mamaeva G, Klebanova E. [Insulin resistance and the role of hormones of adipose tissue in development of diabetes mellitus]. A guide for doctors. 2005.
  42. Nakagawa T, Ukai K, Ohyama T, Gomita Y, Okamura H. Effects of chronic administration of sibutramine on body weight, food intake and motor activity in neonatally monosodium glutamate-treated obese female rats: relationship of antiobesity effect with monoamines. Exp Anim. 2000 Oct;49(4):239-49. CrossRef PubMed
  44. Starovoitova SA, Babenko LP, Timoshok NA, Shynkarenko LN, Lazarenko LN, Spivak NY. Cholesterol-lowering activity of lactic acid bacteria probiotic strains in vivo. Mikrobiol Z. May-Jun;74(3):78-85.
  46. Ooi LG, Liong MT. Cholesterol-lowering effects of probiotics and prebiotics: a review of in vivo and in vitro findings. Int J Mol Sci.11(6):2499-522. CrossRef PubMed PubMedCentral

© National Academy of Sciences of Ukraine, Bogomoletz Institute of Physiology, 2014-2024.