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Insulin determination in pancreas of guinea pigs with metabolic syndrome
V.V. Ruschak, M.O. Chashchyn
Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, Kyiv, Ukraine
DOI: https://doi.org/10.15407/fz60.02.045
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Abstract
The level of insulin production is one of the integral parameters
during the modeling of metabolic syndrome in animals.
Comparison of this parameter with blood glucose level can
characterize the degree of insulin resistance of cells. In this
work we determined the level of insulin in pancreatic cells of
guinea pigs under normal conditions and during metabolic
syndrome. The obtained results were compared with the blood
glucose level of experimental animals. In this work we used
the methods of histochemical and immunohistochemical
analysis of insulin production. It was found that in guinea pigs
metabolic syndrome modeling is accompanied by impaired
insulin synthesis, degenerative changes in pancreatic tissue
and increased blood glucose level. The data obtained suggest
that the experimental animals have insulin resistance, which
is the main pathogenetic mechanism in the development of
metabolic syndrome.
Keywords:
immunohistochemistry, pancreas, guinea pigs,insulin, metabolic syndrome.
References
- Kravets E. Samoilov Y. et al. Metabolic syndrome in a total medicine practice. Bulletin of Siberian medicine. 2008: N 1: 80-87
- Ambrosova T.N., O.N. Kovalyova, T.V. Ascheulova. Role of carbohydrates metabolism disorders and proinflammatory cytokines activity in development of obesity-associated hypertens. Ukr. Cardiol Journ.: 5: 2009.
- Danilova, L. Insulin resistance phenomenon in clinical practice. Lechebnoe delo. 2009: 2 (6): 29-40.
- Bouzakri K., Zierath J. MAP4K4 gene silencing in human skeletal muscle prevents tumor necrosis factoralpha- induced insulin resistance. J Biol Chem. 2007:282(11):7783-9
CrossRef
PubMed
- Chazova I., Mychka V. Metabolic syndrome. Russian Medical Journal. 2004: 163.
- Adiels M., Olofsson S.O., Taskinen M.R. et al. Overproduction of very low-density lipoproteins is the hallmark of the dyslipidemia in the metabolic syndrome. Arterioscler. Thromb. Vasc. Biol. 2008: 28 (7): 1225-1236.
CrossRef
PubMed
- Gordon C. Weirand, Susan Bonner-Weir. Five Stages of Evolving Beta-Cell Dysfunction During Progression to Diabetes. Diabetes. 2004: 53 (3): 16-21
- Haist R., Davidson J. A study of the levels of extractable insulin of guinea pig pancreas. Canadian Journal of Physiology and Pharmacology. 1964: 42(3): 315-317
CrossRef
PubMed
- Smith, Leslie F. Amino Acid Sequences of Insulins. Diabetes. 1972: 21 (2): 457-460
CrossRef
PubMed
- Neubauer, H., Schune, H. The Immunogenicity of Different Insulins in Several Animal Species. Diabetes. 1978: 27 (1): 8-15
CrossRef
PubMed
- Conlon J.M. Evolution of the insulin molecule: insights into structure-activity and phylogenetic relationships. Peptides. 2001: 22(7):1183-93.
CrossRef
- Rushchak V., Kovalenko V. et al. Optimization of animal model for investigation of pathogenesis of metabolic syndrome. Fiziologichnyj Zhurnal. 2012: 58 (6): 29-35
PubMed
- Sarkisov D., Perov J., editors. Microscopically technique. Moscow: Medicine: 1996.
- Kumar G., Rudbeck L., editors. Immunohistochemical staining methods. Fifth Edition. Carpinteria, California: Dako North America: 2009.
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© National Academy of Sciences of Ukraine, Bogomoletz Institute of Physiology, 2014-2024.
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