Українська Русский English

ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2014; 60(2): 45-50


Insulin determination in pancreas of guinea pigs with metabolic syndrome

V.V. Ruschak, M.O. Chashchyn

    Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, Kyiv, Ukraine
DOI: https://doi.org/10.15407/fz60.02.045

Abstract

The level of insulin production is one of the integral parameters during the modeling of metabolic syndrome in animals. Comparison of this parameter with blood glucose level can characterize the degree of insulin resistance of cells. In this work we determined the level of insulin in pancreatic cells of guinea pigs under normal conditions and during metabolic syndrome. The obtained results were compared with the blood glucose level of experimental animals. In this work we used the methods of histochemical and immunohistochemical analysis of insulin production. It was found that in guinea pigs metabolic syndrome modeling is accompanied by impaired insulin synthesis, degenerative changes in pancreatic tissue and increased blood glucose level. The data obtained suggest that the experimental animals have insulin resistance, which is the main pathogenetic mechanism in the development of metabolic syndrome.

Keywords: immunohistochemistry, pancreas, guinea pigs,insulin, metabolic syndrome.

References

  1. Kravets E. Samoilov Y. et al. Metabolic syndrome in a total medicine practice. Bulletin of Siberian medicine. 2008: N 1: 80-87
  2.  
  3. Ambrosova T.N., O.N. Kovalyova, T.V. Ascheulova. Role of carbohydrates metabolism disorders and proinflammatory cytokines activity in development of obesity-associated hypertens. Ukr. Cardiol Journ.: 5: 2009.
  4.  
  5. Danilova, L. Insulin resistance phenomenon in clinical practice. Lechebnoe delo. 2009: 2 (6): 29-40.
  6.  
  7. Bouzakri K., Zierath J. MAP4K4 gene silencing in human skeletal muscle prevents tumor necrosis factoralpha- induced insulin resistance. J Biol Chem. 2007:282(11):7783-9 CrossRef PubMed
  8.  
  9. Chazova I., Mychka V. Metabolic syndrome. Russian Medical Journal. 2004: 163.
  10.  
  11. Adiels M., Olofsson S.O., Taskinen M.R. et al. Overproduction of very low-density lipoproteins is the hallmark of the dyslipidemia in the metabolic syndrome. Arterioscler. Thromb. Vasc. Biol. 2008: 28 (7): 1225-1236. CrossRef PubMed
  12.  
  13. Gordon C. Weirand, Susan Bonner-Weir. Five Stages of Evolving Beta-Cell Dysfunction During Progression to Diabetes. Diabetes. 2004: 53 (3): 16-21
  14.  
  15. Haist R., Davidson J. A study of the levels of extractable insulin of guinea pig pancreas. Canadian Journal of Physiology and Pharmacology. 1964: 42(3): 315-317 CrossRef PubMed
  16.  
  17. Smith, Leslie F. Amino Acid Sequences of Insulins. Diabetes. 1972: 21 (2): 457-460 CrossRef PubMed
  18.  
  19. Neubauer, H., Schune, H. The Immunogenicity of Different Insulins in Several Animal Species. Diabetes. 1978: 27 (1): 8-15 CrossRef PubMed
  20.  
  21. Conlon J.M. Evolution of the insulin molecule: insights into structure-activity and phylogenetic relationships. Peptides. 2001: 22(7):1183-93. CrossRef  
  22. Rushchak V., Kovalenko V. et al. Optimization of animal model for investigation of pathogenesis of metabolic syndrome. Fiziologichnyj Zhurnal. 2012: 58 (6): 29-35 PubMed
  23.  
  24. Sarkisov D., Perov J., editors. Microscopically technique. Moscow: Medicine: 1996.
  25.    
  26. Kumar G., Rudbeck L., editors. Immunohistochemical staining methods. Fifth Edition. Carpinteria, California: Dako North America: 2009.
  27.  

© National Academy of Sciences of Ukraine, Bogomoletz Institute of Physiology, 2014-2019.