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Insulin determination in pancreas of guinea pigs with metabolic syndrome
V.V. Ruschak, M.O. Chashchyn
Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, Kyiv, Ukraine
DOI: https://doi.org/10.15407/fz60.02.045
Abstract
The level of insulin production is one of the integral parameters
during the modeling of metabolic syndrome in animals.
Comparison of this parameter with blood glucose level can
characterize the degree of insulin resistance of cells. In this
work we determined the level of insulin in pancreatic cells of
guinea pigs under normal conditions and during metabolic
syndrome. The obtained results were compared with the blood
glucose level of experimental animals. In this work we used
the methods of histochemical and immunohistochemical
analysis of insulin production. It was found that in guinea pigs
metabolic syndrome modeling is accompanied by impaired
insulin synthesis, degenerative changes in pancreatic tissue
and increased blood glucose level. The data obtained suggest
that the experimental animals have insulin resistance, which
is the main pathogenetic mechanism in the development of
metabolic syndrome.
Keywords:
immunohistochemistry, pancreas, guinea pigs,insulin, metabolic syndrome.
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© National Academy of Sciences of Ukraine, Bogomoletz Institute of Physiology, 2014-2024.
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