Українська Русский English

ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2014; 60(2): 38-44


Expression of XBP1 in lymphocytes of the small intestine in rats under chronic social stress and modulation of intestinal microflora composition

І.A. Topol, A.M. Kamyshny, A.V. Abramov, Yu.M. Kolesnik

    Zaporizhzhia State Medical University, Zaporizhzhia, Ukraine
DOI: https://doi.org/10.15407/fz60.02.038

Abstract

the present study was conducted to investigate of the influence of chronic social stress and modulation of the composition of intestinal microflora on the distribution of Хbp1+-lymphocytes in the gut-associated lymphoid tissue of ileum of the rats. Structure of population of Xbp1+-cells has been studied by the analysis of serial histological sections using the method of indirect immunofluorescenсe with monoclonal antibodies to Xbp1 of rat. Chronic social stress development is accompanied with the reduction of total number of Xbp1+-lymphocytes in lymphoid structures of ileum (31% -3 fold reduction, p<0,05), mostly expressed in lymphoid follicles, and changes the concentration of Xbp1 protein in immunopositive cells. Modulation of the composition of intestinal microflora by antibiotics and probiotics under chronic social stress results in the increase of total number of Xbp1+ lymphocytes in gut-associated lymphoid tissue, the degree of it depends on the kind of stress. The discovered alterations of Xbp1 expression under stress may be one of the triggers for development of autoimmune and inflammatory bowel diseases. Thus, increased understanding of the molecular actions and transcriptional networks regulated by XBP1 in immune cells may aid in the development of potential therapeutics targeting immune disorders.

Keywords: stress, gut-associated lymphoid tissue, transcription factor Xbp1, probiotics, antibiotics.

References

  1. Mays J, Bailey M, Hunzeker J. Influenza virus-specific immunological memory Is enhanced by repeated social defeat. J Immunol. 2010; 184: 2014-2025. CrossRef PubMed PubMedCentral
  2.  
  3. Bailey M, Kierstein S, Haczku A. Social stress enhances allergen-induced airway inflammation in mice and inhibits corticosteroid responsiveness of cytokine production. J.Immunol. 2009; 182: 7888-7896. CrossRef PubMed PubMedCentral
  4.  
  5. Powell N, Bailey M, Mays J. Repeated social defeat activates dendritic cells and enhances Toll-like receptor dependent cytokine secretion. Brain Behav. Immun. 2009; 23: 225-231. CrossRef PubMed PubMedCentral
  6.  
  7. Allen R, Lafuse W, Galley J. The intestinal microbiota are necessary for stressor-induced enhancement of splenic macrophage microbicidal activity. Brain Behav. Immun.2012; 26: 371-382. CrossRef PubMed PubMedCentral
  8.  
  9. Hetz C. The unfolded protein response: controlling cell fate decisions under ER stress and beyond. Nat. Rev. Mol. Cell Biol. 2012; 13: 89-102. CrossRef PubMed
  10.  
  11. Fritz T, Niederreiter L, Adolph T. Crohn's disease: NOD2, autophagy and ER stress converge. Gut. 2011; 60: 1580-1588. CrossRef PubMed PubMedCentral
  12.  
  13. Kaser A, Blumberg R. Endoplasmic reticulum stress and intestinalinflammation. Mucosal Immunology. 2010; 3: 11-16. CrossRef PubMed PubMedCentral
  14.  
  15. Kaser A, Blumberg R. Survive an innate immune response through XBP1. Cell Research.2010; 20: 506-507. CrossRef PubMed PubMedCentral
  16.  
  17. Kaser A, Flak M, Blumberg R. The unfolded protein response and its role in intestinal homeostasis and inflammation. Exp. Cell Res. 2011; 15: 2772-2779. CrossRef PubMed PubMedCentral
  18.  
  19. Brown E, Sadarangani M, Finlay B. The role of the immune system in governing host-microbe interactions in the intestine. Nature Immunology. 2013; 14: 660-667. CrossRef PubMed
  20.  
  21. Avgustinovich D, Kovalenko I. Gender-related characteristics of responding to prolonged psychoemotional stress in mice. Neurosc. Behav. Physiol. 2009; 40 (3): 858-867.
  22.  
  23. Costa C, Rosa S, Camargo M. The Unfolded Protein Response: How Protein Folding Became a Restrictive Aspect for Innate Immunity and B Lymphocytes. Scand. Journal of Immunology. 2011; 73: 436-448. CrossRef PubMed
  24.  
  25. Martinon F, Chen X, Glimcher L. Toll-like receptor activation of XBP1 regulates innate immune responses in macrophages. Nat.Immunol. 2010; 11: 411-418. CrossRef PubMed PubMedCentral
  26.  
  27. Richardson C, Kooistra T, Kim D. An essential role for XBP-1 in host protection against immune activation in C. elegans. Nature. 2010; 463: 1092-1095. CrossRef PubMed PubMedCentral
  28.  
  29. Martinon F, Glimcher L. Regulation of Innate Immunity by signaling pathways emerging from the endoplasmic reticulum. Curr.Opin.Immunol. 2011; 23: 35-40. CrossRef PubMed PubMedCentral
  30.  
  31. Iwakoshi N, Pypaert M, Glimcher L. The transcription factor XBP-1 is essential for the development and survival of dendritic cells. J. Exp. Med. 2007; 204: 2267-2275. CrossRef PubMed PubMedCentral
  32.  
  33. Todd D, Mc Heyzer-Williams L, Glimcher L. XBP1 governs late events in plasma cell differentiation and is not required for antigen-specific memory B cell development. J. Exp. Med. 2009; 206: 2151-2159. CrossRef PubMed PubMedCentral
  34.  
  35. Gass J, Jiang H, Wek R. The unfolded protein response of Blymphocytes: PERK-independent development of antibodysecreting cells. Mol. Immunol. 2008; 45: 1035-1043. CrossRef PubMed PubMedCentral
  36.  
  37. Glimcher L. XBP1: the last two decades. Ann. Rheum. Dis. 2010; 69: 67-71. CrossRef PubMed
  38.  
  39. Franco A, Almanza G, Burns J. Endoplasmic reticulum stress drives a regulatory phenotype in human T-cell clones. Cell Immunol. 2010; 266: 1-6. CrossRef PubMed
  40.  

© National Academy of Sciences of Ukraine, Bogomoletz Institute of Physiology, 2014-2019.