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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2014; 60(2): 38-44

Expression of XBP1 in lymphocytes of the small intestine in rats under chronic social stress and modulation of intestinal microflora composition

І.A. Topol, A.M. Kamyshny, A.V. Abramov, Yu.M. Kolesnik

    Zaporizhzhia State Medical University, Zaporizhzhia, Ukraine
DOI: https://doi.org/10.15407/fz60.02.038


Abstract

the present study was conducted to investigate of the influence of chronic social stress and modulation of the composition of intestinal microflora on the distribution of Хbp1+-lymphocytes in the gut-associated lymphoid tissue of ileum of the rats. Structure of population of Xbp1+-cells has been studied by the analysis of serial histological sections using the method of indirect immunofluorescenсe with monoclonal antibodies to Xbp1 of rat. Chronic social stress development is accompanied with the reduction of total number of Xbp1+-lymphocytes in lymphoid structures of ileum (31% -3 fold reduction, p<0,05), mostly expressed in lymphoid follicles, and changes the concentration of Xbp1 protein in immunopositive cells. Modulation of the composition of intestinal microflora by antibiotics and probiotics under chronic social stress results in the increase of total number of Xbp1+ lymphocytes in gut-associated lymphoid tissue, the degree of it depends on the kind of stress. The discovered alterations of Xbp1 expression under stress may be one of the triggers for development of autoimmune and inflammatory bowel diseases. Thus, increased understanding of the molecular actions and transcriptional networks regulated by XBP1 in immune cells may aid in the development of potential therapeutics targeting immune disorders.

Keywords: stress, gut-associated lymphoid tissue, transcription factor Xbp1, probiotics, antibiotics.

Full text: (PDF, in English)

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