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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2014; 60(2): 12-17

Nitric oxide as a possible regulator of energy-dependent Ca2+ transport in mitochondria of uterine smooth muscle

Danylovych IuV, Kolomiiets' OV, Danylovych HV, Kosterin SO.

    O.V. Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kiev, Ukraine


The influence of the donor and the precursor of NO, namely 100 mM sodium nitroprusside and sodium nitrite on the energodependent Ca2+-transport in isolated mitochondria from rat myometrium was investigated. Changes in the mitochondrial matrix Ca2+-concentration was evaluated by spectrofluorimetry using Ca2+ sensitive probe Fluo-4 AM. Mg2+-ATP-dependent Ca2+-accumulation on mitochondria in the presence of succinate significantly stimulated by nitric oxide, in particular, 100 ?M sodium nitroprusside amplified the transport by 1.6 times relative to its control values. NO effect becomes significant only when the incubation of mitochondria with the compounds was performed. Ca2+-accumulation in the presence of sodium nitroprusside effectively suppressed by protonophore (CCCP) and ruthenium red (10 ?M). It was concluded that inner mitochondrial membrane Ca2+-uniporter stimulated by nitrogen oxide. Ca2+-accumulation in mitochondria in the presence of sodium nitroprusside was not sensitive to the action of a specific permeability transition pore inhibitor cyclosporine (5 ?M). This data indicates that the role of permeability transition pore is less significant than Ca2+-uniporter in the processes of Ca2+- transport in mitochondria under the nitric oxide action. Thus, nitric oxide stimulates the energo-dependent Ca2+-accumulation by myometrium mitochondria mediated their inner membrane Ca2+-uniporter functioning.

Keywords: mitochondria, nitric oxide, calcium, calciumuniporter, myometrium.


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