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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2014; 60(2): 3-11


Pretective action of peptide pro-gly-pro on electrophysiological characteristics of cultured hippocampal neurones during excitotoxic damage

V.Iu. Maslov1, N.S. Veselovs'kyĭ1, A.O. Moskaliuk1, M.F. M'iasoiedov2, S.I. Shram2, S.A. Fedulova1

  1. Bogomoletz Institute of Physiology, NAS of Ukraine, Kyiv, Ukraine
  2. Institute of Molecular genetics, RAS, Moscow, Russia
DOI: https://doi.org/10.15407/fz60.02.003

Abstract

Electrophysiological characteristics of hippocampal neu- rones cultured with Pro-Gly-Pro peptide were studied using glutamate excitotoxisity model (excitotoxic damage was induced by 100 mkM glutamate application during 5 min). It was found that negative changes in neurones cultured with 10 mcM Pro-Gly-Pro were less prominent if compared with control ones. Culturing with the peptide signi?cantly af- fected the following parameters: resting potential (-55±4 mV in control; -29±6 мV after glutamate application; -38±5 мV cultured after glutamate application), action potential am- plitude (91±4; 65±5; 84±5 mV), duration (4,3±0,4; 9,5±1,6; 5,2±0,7 ms), depolarization (56 [38, 84]; 27 [21, 35]; 46 [28, 62] мV/мs) and repolarization (-29 [-38, -27]; -20 [-21, -18]; -29 [-33, -22] мV/мs) rates. The data obtained suggest that PGP exhibit its neuroprotective properties on a level of basic electrophysiological characteristics, appropriate cellular mechanisms require further investigations.

Keywords: hippocampus, neurones culturing, excitotoxicity, neuroprotective action, Pro-Gly-Pro peptide.

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