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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2014; 60(2): 3-11

Pretective action of peptide pro-gly-pro on electrophysiological characteristics of cultured hippocampal neurones during excitotoxic damage

V.Iu. Maslov1, N.S. Veselovs'kyĭ1, A.O. Moskaliuk1, M.F. M'iasoiedov2, S.I. Shram2, S.A. Fedulova1

  1. Bogomoletz Institute of Physiology, NAS of Ukraine, Kyiv, Ukraine
  2. Institute of Molecular genetics, RAS, Moscow, Russia
DOI: https://doi.org/10.15407/fz60.02.003


Abstract

Electrophysiological characteristics of hippocampal neu- rones cultured with Pro-Gly-Pro peptide were studied using glutamate excitotoxisity model (excitotoxic damage was induced by 100 mkM glutamate application during 5 min). It was found that negative changes in neurones cultured with 10 mcM Pro-Gly-Pro were less prominent if compared with control ones. Culturing with the peptide signi?cantly af- fected the following parameters: resting potential (-55±4 mV in control; -29±6 мV after glutamate application; -38±5 мV cultured after glutamate application), action potential am- plitude (91±4; 65±5; 84±5 mV), duration (4,3±0,4; 9,5±1,6; 5,2±0,7 ms), depolarization (56 [38, 84]; 27 [21, 35]; 46 [28, 62] мV/мs) and repolarization (-29 [-38, -27]; -20 [-21, -18]; -29 [-33, -22] мV/мs) rates. The data obtained suggest that PGP exhibit its neuroprotective properties on a level of basic electrophysiological characteristics, appropriate cellular mechanisms require further investigations.

Keywords: hippocampus, neurones culturing, excitotoxicity, neuroprotective action, Pro-Gly-Pro peptide.

Full text: (PDF, in Russian)

References

  1. Kopylova GN, Badmaeva SE, Levitskaya NG, Samonina GE, Umarova BA, Guseva AA. Effect of peptide Pro-Gly-Pro on stress-induced behavioral changes in rats. Bull Exp Biol Med. 2004; 138(1):5-7. CrossRef PubMed
    2.  
  2. Martynova KV, Andreeva LA, Klimova PA, Kirillova IuG, Shevchenko VP, Nagaev IIu, Shram IIu, Shvets VI, Miasoedov NF. Structural-functional study of glycine-and-proline-containing peptides (glyprolines) as potential neuroprotectors. bioorg. Khim. 2009; 35(2):165-171.
    3.  
  3. Stavchanskii VV, Tvorogova TV, Botsina AIu, Skvortsova VI, Limborskaia SA, Miasoedov NF, Dergunova LV. The effect of semax and its C-end peptide PGP on expression of the neurotrophins and their receptors in the rat brain during incomplete global ischemia. Mol. Biol (Mosk) 2011; 45(6):1026-1035.
    4.  
  4. Dmitrieva V.G., Povarova O.V., Skvortsova V.I., Limbo-rska S.A., Myasoedov N.F., Dergunova L.V. Semax and Pro-Gly-Pro activate the transcription of neurotrophins and their receptor genes after cerebral ischemia. Cell. Mol. Neurobiol. 2010; 30(1):71-79. CrossRef PubMed
    5.  
  5. Khodorov B.I. Glutamate-induced deregulation of calcium homeostasis and mitochondrial dysfunction in mammalian central neurons. Prog. Biophys. Mol. Biol. 2004; 86:279-351. CrossRef PubMed
    6.  
  6. Storozhevykh TP, Tukhbatova GR, Senilova YE, Pinelis VG, Andreeva LA, Myasoyedov NF. Effects of semax and its Pro-Gly-Pro fragment on calcium homeostasis of neurons and their survival under conditions of glutamate toxicity. Bull. Exp. Biol. Med. 2007; 143(5):601-604. CrossRef PubMed
    7.  
  7. Fedulova S.A., Vasilyev D.V., Isaeva E.V., Romanyuk S.G., Veselovsky N.S. Possibility of multiquantal transmission at single inhibitory synapse in cultured rat hippocampal neurons. Neurosci 1999; 92(4):1217-1230. CrossRef  
  8. Norris C.M., Blalock E.M., Thibault O., Brewer L.D., Clodfelter G.V., Porter N.M., Landfield P.W. Electrophysiological mechanisms of delayed excitoto-xicity: positive feedback loopbetween NMDA receptor current and depolarization-mediated glutamate release. J. Neurophysiol. 2006; 96(5):2488-2500. CrossRef PubMed PubMedCentral
    9.  
  9. Guo L., Yeh M.L., Cuzon Carlson V.C., Johnson-Venkatesh E.M., Yeh H.H. Nerve growth factor in the hippocamposeptal system: evidence for activity-dependent anterograde delivery and modulation of synaptic activity. J. Neurosci. 2012; 32(22):7701-7710. CrossRef PubMed PubMedCentral
    10.  
  10. Jia Z., Bei J., Rodat-Despoix L., Liu B., Jia Q., Delmas P., Zhang H. J. NGF inhibits M/KCNQ currents and selectively alters neuronal excitability in subsets of sympathetic neurons depending on their M/KCNQ current background. Gen. Physiol. 2008; 131(6):575-587. CrossRef PubMed PubMedCentral
    11.  
  11. V'unova TV, Andreeva LA, Shevchenko KV, Shevchenko VP, Bobrov MY, Bezuglov VV, Myasoedov NF Binding of tripeptide Pro-Gly-Pro labeled at the C-terminal proline residue to plasma membranes of the rat forebrain. Dokl Biol Sci 2008; 419:95-96. CrossRef PubMed
    12.  
  12. Kiedrowski L., Costa E. Glutamate-induced destabilization of intracellular calcium concentration homeostasis in cultured cerebellar granule cells: role of mitochondria in calcium buffering. Mol. Pharmacol. 1995; 47(1):140-147. PubMed
    13.  
  13. Dargent B, Couraud F. Down-regulation of voltage-dependent sodium channels initiated by sodium influx in developing neurons. Proc. Nat. Acad. Sci. USA. 1990;87(15):5907-5911. CrossRef  
  14. Marini A.M., Jiang X., Wu X., Pan H., Guo Z., Mattson M.P.,BlondeauN., Novelli A., Lipsky R.H. Preconditioningand neurotrophins: a model for brain adaptation toseizures, ischemia and other stressful stimuli. AminoAcids 2007; 32(3):299-304. CrossRef PubMed
    15.    

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