Українська English

ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2014; 60(1): 49-55

Frequency of allele polymorphism of immune proteasome catalytic subunits in patients with ischemic stroke

Savchuk OI1, Mel'nyk VS1, Goncharov SV1, Shandiuk VIu1, Stroĭ DO1, Dosenko VIe1, Sokolova LI1, Skybo HH1,2

  1. O.O. Bogomoletz Institute of Physiology National Academy of Science of Ukraine, Kyiv, Ukraine
  2. State Key Laboratory of Molecular and Cellular Biology, Ukraine
DOI: https://doi.org/10.15407/fz60.01.049


Abstract

Frequency of allelic variants of proteasome subunits genes LMP2 (Arg60>His) and PSMA6 were determined in patients with ischemic stroke using real-time PCR. Allelic variants of PSMA6 were disposed in the next manner: C/С – 80,2%, С/G – 19,8%, G/G – were not (in control) and C/С – 75,5%, С/G – 21,4%, G/G – 3,1% (Р=0,22) in patients with IS. It was shown that distribution of LMP2 allelic variants was the following: Arg/Arg – 53,3%, Arg/His – 43,5%, His/His – 6,7% in control and Arg/Arg – 55,9%, Arg/His – 34,3%, His/His – 9,8% in IS group (P>0,05). The data show that LMP2 and PSMA6 gene polymorphism is not a risk factor of ischemic stroke in Ukrainian population.

Keywords: proteasome, single nucleotide polymorphism, ischemic stroke.

Full text: (PDF, in Ukrainian)

References

  1. Barbieri M, Marfella R, Rizzo MR, Boccardi V, Siniscalchi M, Schiattarella C, Siciliano S, Lemme P, Paolisso G. The 8UTR C/G polymorphism of PSMA6 gene is associated with susceptibility to myocardial infarction in type 2 diabetic patients. Atherosclerosis. 2008; 201: 117- 123. CrossRef PubMed
    2.  
  2. Bennett DA, Xu P, Clarke R, Zondervan K, Parish S, Palmer A, Cardon L, Peto R, Lathrop M, Collins R. The exon 1-8C/G SNP in the PSMA6 gene contributes only a small amount to the burden of myocardial infarction in 6946 cases and 2720 controls from a United Kingdom population. Eur J Hum Genet. 2008; 16: 480-486. CrossRef PubMed
    3.  
  3. Brass LM, Isaacsohn JL, Merikangas KR, Robinette CD. A study of twins and stroke. Stroke. 1992; 23(2): 221-223. CrossRef PubMed
    4.  
  4. Cheng YC, Anderson CD, Bione S, Keene K, Maguire JM, Nalls M, Rasheed A, Zeginigg M, Attia J, Baker R, Barlera S, BifR A, Bookman E, Brott TG, Brown RDJr, Chen F, Chen WM, Ciusani E, Cole JW, Cortellini L, Danesh J, Doheny K, Ferrucci L, Grazia Franzosi M, Frossard P, Furie KL, Golledge J, Hankey GJ, Hernandez D, Holliday EG, Hsu FC, Jannes J, Kamal A, Khan MS, Kittner SJ, Koblar SA, Lewis M, Lincz L, Lisa A, Matarin M, Moscato P, Mychaleckyj JC, Parati EA, Parolo S, Pugh E, Rost NS, Schallert M, Schmidt H, Scott RJ, Sturm JW, Yadav S, Zaidi M, Boncoraglio GB, Levi CR, Meschia JF, Rosand J, Sale M, Saleheen D, Schmidt R, Sharma P, Worrall B, Mitchell BD. Are myocar- dial infarction-associated single-nucleotide polymorphisms associated with ischemic stroke? Stroke. 2012; 43: 980-986. CrossRef PubMed PubMedCentral
    5.  
  5. Deng GY, Muir A, Maclaren NK, She JX. Association of LMP2 and LMP7 genes within the major histocompatibility complex with insulin-dependent diabetes mellitus: population and family studies. Amer. J. Hum. Genet. 1995; 56: 528-534. PubMed PubMedCentral
    6.  
  6. Dosenko VE, Mykhalchuk DV, Zagoryi VY, Khaytovych MV, Moibenko AA. Functional signiR cance of allelic polymorphisms of genes encoding the catalytic subunit imunoproteasome. Fiziol Zh. 2005; 51(6): 3-10. PubMed
    7.  
  7. Freilinger T, Bevan S, Ripke S, Gschwendtner A, Lichtner P, M'ller-Myhsok B, Wichmann HE, Markus HS, Meit-inger T, Dichgans M. Genetic variation in the lymphotoxin-alpha pathway and the risk of ischemic stroke in European populations. Stroke. 2009; 40: 970-972. CrossRef PubMed
    8.  
  8. Goldberg AL, Cascio P, Saric T, Rock KL. The importance of the proteasome and subsequent proteolytic steps in the generation of antigenic peptides. Mol. Immunol. 2002; 39(3): 147-164. CrossRef  
  9. Goldberg A, Eledzh C, Harper DzhV. Mechanisms of cell death. The world of science. 2001; 2: 32-37.
    10.  
  10. Mishchenko TS. Treatment of stroke: towards full use of the opportunities of the therapeutic window. Health Protection of Ukraine. 2009; 1: 12-15.
    11.  
  11. Morgan TM, Krumholz HM, Lifton RP, Spertus JA. Nonvalidation of reported genetic risk factors for acute coronary syndrome in a large-scale replication study. JAMA. 2007; 297: 1551-1561.
    12.  
  12. Ozaki K, Sato H, Iida A. A functional SNP in PSMA6 confers risk of myocardial infarction in the Japanese population. Nat. Genet. 2006; 38(8): 921-925. CrossRef PubMed
    13.  
  13. Roger VL, Go AS, Lloyd-Jones DM, Benjamin EJ, Berry JD, Borden WB, Bravata DM, Dai S, Ford ES, Fox CS, Fullerton HJ, Gillespie C, Hailpern SM, Heit JA, Howard VJ, Kissela BM, Kittner SJ, Lackland DT, Lichtman JH, Lisabeth LD, Makuc DM, Marcus GM, Marelli A, Matchar DB, Moy CS, Mozaffarian D, Mussolino ME, Nichol G, Paynter NP, Soliman EZ, Sorlie PD, Sotoodehnia N, Turan TN, Virani SS, Wong ND, Woo D, Turner MB. Executive summary: heart disease and Stroke statistics 2012 update: a report from the American Heart Association. Circulation. 2012; 125: 188-197. CrossRef PubMed
    14.  
  14. Skvortsova VI, Yarygin VN, Yarygin KN, Pirogov AY, Gubskiy LV, Uchevatkin AA, Glushkova TT. Experimental model of focal cerebral ischemia in rats based endovascular microsurgery. Zh. Neurology and Psychiatry by SS Korsakov. 2008; 109(2): 62-69.
    15.  
  15. Sjakste T, Poudziunas I, Ninio E, Perret C, Pirags V, Nicaud V, Lazdins M, Evanss A, Morrison C, Cambien F, Sjakste N. SNPs of PSMA6 gene investigation of possible as-sociation with myocardial infarction and type 2 diabetes mellitus. Genetik. 2007; 43: 553-559.
    16.  
  16. Willerson JT, Ridker PM. InIammation as a cardiovascular risk factor. Circulation. 2004; 109: 2-10. CrossRef PubMed
    17.  
  17. Zhang W, Meng H, Li ZH, Shu Z, Ma X, Zhang BX. Regulation of STIM1, store-operated Ca2+ inIux, and nitric oxide generation by retinoic acid in rat mesangial cells. Amer. J.  Physiol. Renal. Physiol. 2007; 292(3): 1054-1064. CrossRef PubMed
    18.  

© National Academy of Sciences of Ukraine, Bogomoletz Institute of Physiology, 2014-2025.