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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2014; 60(1): 25-33


Effects of Lys-form of plasminogen on platelet actin cytoskeleton

Tykhomyrov AO, Zhernosiekov DD, Roka-Moĭia IaM, Diordiieva SI, Hrynenko TV.

    Palladin Institute of Biochemistry of NAS of Ukraine, Kyiv, Ukraine
DOI: https://doi.org/10.15407/fz60.01.025

Abstract

The effects of several forms of plasminogen on the state of actin cytoskeleton of human platelets were studied. A ratio between various actin pools, which were detected by immu- noblotting, was taken as indicator of platelet cytoskeleton reor- ganization. It was revealed that intact platelets contain globular (G) actin and membrane cortex (MC) actin in amounts that are 56 and 40% of Ilamentous (F) actin level, respectively. In both thrombin- and collagen-activated platelets, actin is almost entirely presented in F-form. Incubation of resting platelets with Lys-plasminogen causes elevation of MC-actin level up to 79% in respect to F-form content. In addition, Lys-plasmin- ogen inhibits reorganization of actin cytoskeleton typical for activated platelets. In contrast to Lys-form, Glu-plasminogen affects neither platelet aggregation nor redistribution of actin pools. Thus, these data indicate that cytoskeletal structures of platelets are involved in realization of anti-aggregating effects of Lys-plasminogen.

Keywords: plasminogen, platelets, platelet aggregation, actin, cytoskeleton.

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