Українська Русский English

ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2013; 59(5): 3-10


Using serial tachograms to measure the evoked impulse activity of isolated hippocampal neurons

Iavors'kyĭ VA, Luk"ianets' OO.

    O.O. Bogomoletz Institute of Physiology National Academyof Science of Ukraine, Kyiv, Ukraine
DOI: https://doi.org/10.15407/fz59.05.003

Abstract

In these studies, we investigated the phenomenon of change in impulse activity of isolated hippocampal neurons during longtime recording. We described the use of serial tachograms during registering the electrical activity of neurons, analysis of which can improve the reliability of data. An analysis of the data identified three phases of changes in impulse activity of isolated neurons in the experimental registrations: a phase of increased activity, phase of stable activity and the phase of declining activity. It is established that in conditions of the perforated patch-clamp the phase of stable activity started at 10-15 minutes after formation of the tight junction and had an average duration of 30 minutes. It is shown that the use of the serial tahograms and phases of activity improves the quality of assessment in the measurement of the electrical activity of neurons.

Keywords: hippocampal neurons, perforated patch-clamp, action potential, hippocampus, accommodation, inter-pulse interval

References

  1. Haag J., Borst A. Active membrane properties and signal encoding in graded potential neurons . J.Neurosci. 1998. 18. P. 7972-7986. CrossRef PubMed
  2.  
  3. Iavorskii V.A., Luk'ianets E.A. [Role of calcium ions in frequency accomodation of rat hippocampal neurons] . Ukr.Biokhim.Zh. 2001. 73. P. 123-127.
  4.  
  5. Izhikevich E.M. Simple model of spiking neurons . IEEE Trans.Neural Netw. 2003. 14. P. 1569-1572. CrossRef PubMed
  6.  
  7. Korn S.J., Marty A., Connor J.A.,Horn R. Perforated patch recording . Methods in Neuroscience 1991. 4. P. 364-373. CrossRef  
  8. Nirenberg S., Latham P.E. Decoding neuronal spike trains: how important are correlations? . Proc.Natl.Acad. Sci.U.S.A 2003. 100. P. 7348-7353. CrossRef PubMed PubMedCentral
  9.  
  10. Oram M.W., Wiener M.C., Lestienne R.,Richmond B.J. Stochastic nature of precisely timed spike patterns in visual system neuronal responses . J.Neurophysiol. 1999. 81. P. 3021-3033. CrossRef PubMed
  11.  
  12. Shinomoto S., Shima K.,Tanji J. Differences in spiking patterns among cortical neurons . Neural Comput. 2003. 15. P. 2823-2842. CrossRef PubMed
  13.  
  14. Yavorskii V., Kostyuk P.,Lukyanetz E. Accommodation properties of isolated hippocampal neurons under conditions of an experimental model of epilepsy . Neurophysiology- 2006. 38. P. 175-181. CrossRef  
  15. Yavorskii V.A., Lukyanetz E.A. Evoked impulse activity of isolated hippocampal neurons in the perforated patch-clamp configuration . Neurophysiology 2012. 43. P. 417-424. CrossRef  
  16. Yavorsky V.A., Lukyanetz E.A. An effect of mitochondrial ionophore CCCP on impulse activity of isolated CA1 hippocampal neurons . Fiziol.Zh. 2008. 54. P. 124. 

© National Academy of Sciences of Ukraine, Bogomoletz Institute of Physiology, 2014-2019.