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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2013; 59(4): 67-73


Stress-induced changes in the content of cytochrome P450 2E1 in the liver of mice with chronic psychoemotional overexertion

Maksymchuk OV, Chashchyn MO.

    Institute of Molecular Biology and Genetics of National Academy of Sciences of Ukraine, Kyiv, Ukraine
DOI: https://doi.org/10.15407/fz59.04.067

Abstract

IIn this work we investigated changes in the cytochrome P450 2E1 (CYP2E1) expression level in the liver of mice that have been exposed to psycho-emotional stress (PES). It was shown twofold relative to control reduction of the enzyme, which does not normalize after termination of the stressor. The changes in level of Cyp2e1 mRNA is not observed at any time point of the experiment. At the same time it was found a significant decrease in the expression level of hsp90--one of the factors determines of the level of CYP2E1 degradation in the cell. Also it was shown, the oxidative stress develops in the liver of experimental animals, and this is indicated by a 3-fold increase in the malondialdehyde level on the background of a 5-fold decrease in catalase activity. A decrease in the level of expression of cytochrome P450 2E1 in the liver of mice that have been exposed to psycho-emotional stress is due to the intensification of the peroxide process and the development of oxidative stress. Reduction of protein content of CYP2E1 is apparently not related to the hsp90-dependent processes of its degradation in the cell.

Keywords: catalase, cytochrome P450 2E1, CYP2E1, Cyp2e1 mRNA, hsp90, malondialdehyde, oxidative stress, psychoemotional stress

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