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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2013; 59(4): 56-62

Functional morphology of nucleated erythrocytes during hypoxia (in vitro experiments)

Andrieieva OIu, Soldatov OO

    A.O. Kovalevsky Institute of Biology of the Southern Seas,National Academy of Sciences of Ukraine, Sevastopol, Ukraine


Changes in morphologic and functional properties of nucleic erythrocytes were observed during experimental hypoxia in vitro. It has been shown that erythrocytes volume and nuclei volume decrease in oxygen concentration of 1.76-4.03 mgO2 x l(-1) on 1.5-5.0% and 9-15% respectively. Both indexes increased significantly in case of deep hypoxia (0.57-1.76 mgO2 x l(-1)) when nucleo-cytoplasmic ratio doesn't change. By this time erythrocytes shape modifies into extended ellipse because of elongation of large axis of cell (C1) and its width (h). Along with increase in nuclei volume the enhancement of SYBR Green I fluorescence is observed; the intensity correlates with volume magnitude of this cell structure (R2 = 0.81).

Keywords: hypoxia, in vitro experiments, nucleated erythrocytes, cell morphology, cytometry


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