Functional morphology of nucleated erythrocytes during hypoxia (in vitro experiments)
Andrieieva OIu, Soldatov OO
A.O. Kovalevsky Institute of Biology of the Southern Seas,National Academy of Sciences of Ukraine, Sevastopol, Ukraine
DOI: https://doi.org/10.15407/fz59.04.056
Abstract
Changes in morphologic and functional properties of nucleic erythrocytes were observed during experimental hypoxia in vitro. It has been shown that erythrocytes volume and nuclei volume decrease in oxygen concentration of 1.76-4.03 mgO2 x l(-1) on 1.5-5.0% and 9-15% respectively. Both indexes increased significantly in case of deep hypoxia (0.57-1.76 mgO2 x l(-1)) when nucleo-cytoplasmic ratio doesn't change. By this time erythrocytes shape modifies into extended ellipse because of elongation of large axis of cell (C1) and its width (h). Along with increase in nuclei volume the enhancement of SYBR Green I fluorescence is observed; the intensity correlates with volume magnitude of this cell structure (R2 = 0.81).
Keywords:
hypoxia, in vitro experiments, nucleated erythrocytes, cell morphology, cytometry
References
- Novitskaya V.N., Soldatov A.A., Parfenova I.A. Funktsi onal'naya morfologiya, sopryazhenie membrannih i metabolicheskih funktsii u yadernih eritrotsitov Scorpaena porcus L. v usloviyah eksperimental'noi gipoksii . Dop. NAN Ukraini. 2011. N 10. P. 131-136.
- Parfenova I.A., Soldatov A.A. Funktsional'naya morfologiya tsirkuliruyushchih eritrotsitov bichka-kruglyaka v usloviyah eksperimental'noi gipoksii . Morskoi ekol. zhurn. 2011. 10, N 2. P. 59-67.
- Soldatov A.A., Rusinova O.S., Trusevich V.V., Zvezdina T. F. Vliyanie gipoksii na biohimicheskie pokazateli eritrotsitov skorpeni . Ukr. biohim. zhurn. 1994. 66, N 5. P. 115-118.
- Tashke K. Vvedenie v kolichestvennuyu tsito-gistologicheskuyu morfologiyu. Buharest: Izd-vo Akademii Respubliki Ruminii, 1980. 291 p. Chizhevskii A.L. Strukturnii analiz dvizhushcheisya krovi. M.: Izd-vo AN SSSR, 1959. 474 p.
- Adragna N.C., Di Fulvio M., Lauf P.K. Regulation of K-Cl cotransport: from function to genes . J. Membrane Biol. 2004. 201. P. 109-137.
CrossRef
PubMed
- Borgese F., Garcia-Romeu F. Motais R. Control of cell volume and ion transport by ?-adrenergic catecholamines in erythrocytes of rainbow trout, Salmo gairdneri . J. Physiol. 1987. 382. P. 123-144.
CrossRef
PubMed PubMedCentral
- Boutilier R.G., Ferguson R.A. Nucleated red cell function: metabolism and pH regulation . Can. J. Zool. 1989. 67, N 12. P. 2986-2993.
CrossRef
- Cerca F., Trigo G., Correia A., Cerca N., Azeredo J, Vilanova M. SYBR green as a fluorescent probe to evaluate the biofilm physiological state of Staphylococcus epidermidis, using flow cytometry . Can. J. Microbiol. 2011. 57, N 10. P. 850-856.
CrossRef
PubMed
- Cossins A. R., Gibson J. S. Volume-sensitive transport systems and volume homeostasis invertebrate red blood cells . J. Exp. Biol. 1997. 200. P. 343-352.
PubMed
- Gilles C., Motais R. Effect of catecholamines on deformability of red cells from trout: relative roles of cyclic AMP and cell volume . J. Physiol. 1989. 412. P. 321-332.
CrossRef
- Girish V., Vijayalakshmi A. Affordable image analysis using NIH Image/ImageJ . Indian J. Cancer. 2004. 41, N 1. P. 41-47.
- Hallman T. M., Rojas-Vargas A. C., Jones D. R., Richards J. G. Differential recovery from exercise and hypoxia exposure measured using 31Pand 1H-NMR in white muscle of the common carp Cyprinus carpio . J. Exp. Biol. 2008. 211. P. 3237-3248.
CrossRef
PubMed
- Houchin D.N., Munn J.I., Parnell B.L. A method for the measurement of red cell dimensions and calculation of mean corpuscular volume and surface area . Blood. 1958. 13. P. 1185-1191.
PubMed
- Jahns R., Borgese F., Lindenthal S, Straub A., Motais R., Fievet B. Trout red blood cell arrestin (TRCarr), a novel member of the arrestin family: cloning, immunoprecipitation and expression of recombinant TRCarr . Biochem. J. 1996. 316, N 2. P. 497-506.
CrossRef
PubMed PubMedCentral
- Jensen F. B. Regulatory volume decrease in carp red blood cells: mechanisms and oxygenation-dependency of volume-activated potassium and amino acid transport . J. Exp. Biol. 1995. 198. P. 155-165.
PubMed
- Jensen F.B. Red blood cell pH, the Bohr effect, and other oxygenation-linked phenomena in blood O2 and CO2 transport (review) . Acta Physiol. Scand. 2004. 182, N 3. P. 215-227.
CrossRef
PubMed
- Jensen F.B.; Weber R. E. Kinetics of the acclimational responses of tench to combined hypoxia and hypercapnia . J. Comp. Physiol., B. 1989. 156, N 2. P. 197-203.
CrossRef
- Motais R., Borgese F., Fievet B. Garcia-Romeu F. Regulation of Na+/H+ exchange and pH in erythrocytes of fish . Comp. Biochem. Physiol. 1992. 102a, N 4. P. 597-602.
CrossRef
- Perry S.F., Montpetit C.J., Julio A.E., Moore K. The influence of chronic anaemia on catecholamine secretion in the rainbow trout (Oncorhynchus mykiss) . J. Comp. Physiol. 1999. 169, N 4/5. P. 335-343.
CrossRef
- Phillips M.C.L., Moyes C.D., Tufts B.L. The effects of cell ageing on metabolism in rainbow trout (Oncorhynchus mykiss) red blood cells . J. Exp. Biol. 2000. 203, N 6. P. 1039-1045.
PubMed
- Reid S.D., Perry S.F. The effects of hypoxia, in vivo, on red blood cell b-adrenoreceptors in the rainbow trout, Oncorhynchus mykiss . Fish Physiol. Biochem. 1995. 14, N 6. P. 217-240.
CrossRef
PubMed
- Richards J. G., Wang Y. S., Brauner C. J., Gonzalez R. J., Patrick M. L., Schulte P. M., Choppari-Gomes A. R., Almeida-Val V. M., Val A. L. Metabolic and ionoregulatory responses of the Amazonian cichlid, Astronotus ocellatus, to severe hypoxia . J. Comp. Physiol B. 2007. 177. P. 361-374.
CrossRef
PubMed
- Soldatov A.A. The effect of hypoxia on red blood cells of flounder: a morphologic and autoradiographic study . J. Fish. Biol. 1996. 48, N 3. P. 321-328.
CrossRef
- Tetens V., Lykkeboe G., Christensen N.J. Potency of adrenaline and noradrenaline for b-adrenergic proton extrusion from red cells of rainbow trout, Salmo gairdneri . J. Exp. Biol. 1988. 134. P. 267-280.
PubMed
- Tiihonen K., Nikinmaa M. Short communication substrateutilization by carp (Cyprinus carpio) erythrocytes . J. Exp. Biol. 1991. 161. P. 509-514.
- Walsh P. J., Wood C. M., Thomas S., Perry S. F. Characterization of red blood cell metabolism in rainbow trout. Ibid. 1990. 154. P. 475-489.
- Wells R.M.G. Blood-gas transport and hemoglobin function: adaptations for functional and environmental hypoxia . Fish. Physiol. 2009. 27. P. 255-299.
CrossRef
- Wood C.M., Simmons H. The conversion of plasma HCO3to CO2 by rainbow trout red blood cells in vitro: adrenergic inhibition and the influence of oxygenation status . Fish. Physiol. and Biochem. 1994. 12, N 6. P. 445-454.
CrossRef
PubMed
- Zipper H., Brunner H., Bernhagen J., Vitzthum F. Investigations on DNA intercalation and surface binding by SYBR Green I, its structure determination and methodological implications . Nucleic Acids Res. 2004. 32, N 12. P. e103.
CrossRef
PubMed PubMedCentral
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