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Influence of l-arginine and blockator nitric oxudesynthase l-nna on antioxidant enzymes activity, lipidperoxidation in blood and tissues of rats withdifferent resistance to hypoxia under stressconditions
N. M. Kurhalyuk
Ivan Franko Lviv National UniversityMinistry of Education
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Abstract
The main components of antioxidant enzyme system (AOS) are superoxid dismutase
( SOD) and glutathione reductase (GR) catalyses the conversion of the superoxide
anion. The important role in AOS belongs to catalase and glutathione peroxidase
which perform H2O2 to nontoxic products. Simultaneous determination of AOS
activity and malonic dialdehide (MDA) concentration (the index of lipid peroxidation
in tissues and blood) characterize cells complex resistance to damage factor. The
Н. М. Кургалюк
58 ISSN 0201-8489 Фізіол. журн., 2001, Т. 47, № 2
effect of L-arginine, as a precusor of nitric oxide synthesis and blocator NO-synthase
(Nw-nitro-L-arginine) on AOS of rats with different resistance to hypoxia under
stress condition is unknown and were subject of our investigation. Experiments were
done on liver and blood tissues of white laboratory rats. The experimental animals
were divided on two groups depending on hypoxia factor: high resistance (HR) and
low resistance (LR). The type of resistance was determined by the time of ability to
respire in barocamera with oxygen deficient equal to 12.000 meters over sea level. The
animals adaptation to laboratory conditions continue during 14 days after in
barocamera presence. All animals were divided dependent on experiment conditions
on fourth groups.
The first group: intact (HR and LR) animals parentherally injected by 1 ml of 0.9 %
NaCl solution. The second group was subject of stress condition. The third group:
HR and LR animals injected parentherally by 1 ml L-arginine (Sigma, USA) dose
(600mg /kg body weight). The fourth one: rats injected by 1 ml Nw-nitro-L-arginine
(L-NNA, Sigma, USA)- the blocator of NO-synthase. The animals were decapitated
30 min after injection and stress condition under ethereal anesthesia. Activity of
antioxidant system enzymes superoxid dismutase (SOD), catalase (CAT); glutathione
reductase (GR), glutathione peroxidase (GP) were measured spectrophotometrically.
Also was investigated the concentration of serum antioxidant ceruloplasmin (CP).
Level of lipid peroxidation was estimate by examination of concentration of lipids of
hydroperoxides (LHP) and malonic dialdehyde (MDA). Our data confirm suggesting
that nitric oxide (NO) is a major regulator in the AOS enzymes activity and limit
damage influence of AOF. Action precursor NO L-arginine might be capable of
protective role in various disorders which are connected with hypoxia factor. Following
thing can be interred the investigation of influence of nitric oxide adaptive answears
in stress condition modelling of pathological processes in rats with different resistance
to hypoxia and reflect the biological qualities data stady on AOZ and LP.
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