Українська English

ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2013; 59(4): 28-32

The effect of Ca2+ on the properties of the large conductance cation channels of the nuclear envelope of the cerebellar neurons

Lun'ko OV, Fedorenko OA, Marchenko SM

    O.O. Bogomoletz Institute of Physiology, National Academy of Science of Ukraine, Kyiv, Ukraine


Previously we have found the large conductance cation channels (LCCC) in the nuclear membranes, where inositol-1,4,5-triphosphate receptors (IP3Rs) were also observed. Probably IP3Rs and LCCC are functionally connected: LCCC may provide the counterflow of K+, which prevent the formation of the negative potential in the lumen of the nuclear envelope and in such way may prolong the Ca2+ releasing by IP3Rs. LCCC are poorly studied and their molecular nature is still unknown. We investigated the effect of Ca2+ on properties of these channels. Our results demonstrated the main biophysical properties of LCCC changed significantly neither in Ca(2+)-free solution, nor with high concentrations of Ca2+ in the nuclear lumen. So, the level of Ca2+ repletion of the store does not influence the activity of LCCC.

Keywords: nuclear envelope, ion channels, calcium store


  1. Fedorenko O.A., Duzhyy D.E., Marchenko S.M. Cationic large conductance channels of the nu clear envelope of Purkinje neurons of cerebellum . Neurophysiology. 2007. 39, N 2. P.13-18. CrossRef  
  2. Fedorenko O.A., Marchenko S.M. The meaning of cation channels in the function of NE as Sa2+ store . Neurophysiology. 2010. 42, N 4. P.281-286.
  4. Fedorenko O.A., Yarotskyy V, Duzhyy D.E., Marchenko S.M. The large conductance ion channels in the nuclear envelope of central neurons . Pflug. Arch. 2010. 460. P. 1045-1050. CrossRef PubMed
  6. Fedorenko O.A., Marchenko S.M. Spontaneously Active Ion Channels of the Nuclear Envelope Membrane . Int. J. of Physiol. & Pathophysiol. 2011. 2, N 2. P.183-195.
  8. Fedorenko O.A., Marchenko S.M. Properties of LargeConductance Cationic Channels in the Neuronal Nuclear Envelope . Neurophysiology. 2011. 43, N 3. P. 192-194. CrossRef  
  9. Franco-Obergon A., Wang H., Clapham D.E. Distinct ion channel classes are expressed on the outer nuclear envelope of Tand B-lymphocyte cell lines . Biophys. J. 2000. 79. P. 202-214. CrossRef  
  10. Humbert J.P., Matter N., Artault J.C., Koppler P., Malviay A.N. Inositol 1,4,5-trisphosphate receptor is located to the inner nuclear membrane vindicating regulation of nuclear calcium signaling by inositol 1,4,5-trisphosphate . J. Biol. Chem. 1996. 271, N . 1. P.478-485.
  12. Latorre R, Brauchi S. Large conductance Ca2+-activated K+ (BK) channel: activation by Ca2+ and voltage . Biol. Res. 2006. 39, N 3. P.385-401. CrossRef PubMed
  14. Marchenko S.M., Yarotskyy V.V., Kovalenko T.N., Kostyuk P.G., Thomas R.C. Spontaneously active and InsP3activated ion channels in cell nuclei from rat cerebellar Purkinje and granule neurons . J. Physiol. 2005. 565 N 15. P.897-910. CrossRef PubMed PubMedCentral
  16. Mazzanti M, DeFelice L.J., Cohn J., Malter H. Ion channels in the nuclear envelope . Nature. 1990. 22, N 343(6260). p. 764-767.
  18. Mazzanti M., Bustamante J.O., Oberleithner H. Electrical dimension of the nuclear envelope . Physiol. Rev. 2001. 81, N 1. P.1-19. CrossRef PubMed
  20. Picard L., Cote K., Teijeira J., Greentree D, Rousseau E. Sarcoplasmic reticulum K+ channels from human and sheep atrial cells display a specific electro-pharmacological profile . J. Mol. Cell Cardiol. 2002. 34. p. 1163-1172. CrossRef PubMed
  22. Yamashita M., Sugioka M., Ogawa Y. Voltageand Ca2+activated potassium channels in Ca2+ store control Ca2+ release . FEBS J. 2006. 273. p. 3585-3597. CrossRef PubMed

© National Academy of Sciences of Ukraine, Bogomoletz Institute of Physiology, 2014-2024.