Functional state of the ileum, cationic peptids and zinc content in the Paneth cells of the rats following lincomycin, indomethacin and methotrexate andministration
Horokhovs'kyĭ IeIu, Ieshchenko IuV, Bovt VD, Ieshchenko VA, Iurchuk IIe
Zaporozhye National University, Ukraine
DOI: https://doi.org/10.15407/fz59.03.078
![](/images/cite.png)
Abstract
In the paper the relation between the changes of a functional condition of ileum, composition of its microflora and secretory activity of Paneth cells has been investigated. Administration of lincomycin led to a reduction of total of symbiotic bacteria and appearance of opportunistic microflora. Statistically insignificant changes of speed of transit of intestinal contents and damage of barrier function of an intestinal epithelium were noted. The intestinal epithelium was characterized by signs of inflammation. Administration of indomethacin and methotrexate led to significant changes of a functional state of a small intestine of rats. The speed of transit of intestinal contents following indomethacin administration increased by 30%, and, on the contrary, decreased by 90% following administration of methotrexate. The ability of epithelium of ileum to adsorb vital dye in both cases increased (by 32 and 51%, respectively) indicating the damage of barrier function. Thus there were significant changes in structure of microflora of ileum: reduction of quantity symbiotic and emergence of opportunistic microflora, a translocation of bacteria to a mucous wall. It was concluded that animal of all three experimental groups had a reduction of the contents of cationic proteins and zinc in the secretory granules of Paneth cells. These results can testify that balance changes between simbiotic and opportunistic microflora are a factor which has an impact on intestine damage and also causes activation of secretory function of Paneth cells.
Keywords:
dysbacteriosis, enteritis, cationic proteins, Panethcells, zinc.
References
- Denisov S.D., Morozkina T.S. Trebovaniya k nauchnomu eksperimentu s ispol'zovaniem zhivotnih . Zdravoohranenie. 2001. N 4. P. 40-42.
- Ieshchenko Yu.V. Stres i metabolizm metaliv. Zaporizhzhya: Vid-vo Zaporiz. nats. un-tu, 2010. 258 p.
PubMed
- Ieshchenko Yu.V., Ieshchenko V.A., Bovt V.D. Vmist tsinku, shcho utvoryuie helati, ta sekretornogo materialu v granulotsitah krovi ta bazal'nih viddiliv kishkovih kript pri stresi . Fiziol. zhurn. 2010. 56, N 5. P. 40-44.
- Eshchenko V.A. Gistohimicheskoe issledovanie tsinka . Tsitologiya. 1978. 20, N 8. P. 927-933.
- Znamenskii V.A., Degtyar N.V., Kuz'minskii S.N. Metodicheskie rekomendatsii. Mikrobiologicheskaya diagnostika disbakteriozov. K. : Izd-vo Kiev. gos. un-ta usovershenstvovaniya vrachei, 1986. 27 p.
- Pat. N 31012, UA. MPK (2006) A61R31/00. Sposib modelyuvannya disbiozu (disbakteriozu). Levits'kii A.P., Selivans'ka I.O., Tsisel's'kii Yu.V., Pochtar V.M., Rozsohanova L.M., Gulavs'kii V.T. ? 2008, Byul. N 6.
- Rebrova O.Yu. Opisanie protseduri i rezul'tatov statisticheskogo analiza meditsinskih dannih v nauchnih publikatsiyah. V kn.: Rekomendatsii po podgotovke nauchnih meditsinskih publikatsii. Pod red. S.E. Bashchinskogo, V.V. Vlasova. M.: MediaSfera, 2006. P. 94-105
- Ayabe, T., Satchell, D. P., Wilson, C. L., Parks, W. C., Selsted, M. E., Ouellette, A. J. Secretion of microbicidal ?-defensins by intestinal Paneth cells in response to bacteria . Nat. Immunol. 2000. 1, N 2. P. 113-118.
CrossRef
PubMed
- Bevins C. L. Paneth cell defensins: key effector molecules of innate immunity . Biochem. Soc. Trans. 2006. 34.P. 263-266.
CrossRef
PubMed
- Bidgood M.J., Jamal O.S., Cunningham A.M., BrooksP.M. Type IIA secretory phospholipase A2 up-regulatescyclooxygenase-2 and amplifies cytokine-mediated prostaglandinproduction in human rheumatoid synoviocytes. J. Immunol. 2000. 165, N 5. P. 2790-2797.
CrossRef
PubMed
- Cohen P.S., Laux D.C. Bacterial adhesion to and penetrationof intestinal mucus in vitro . Methods Enzymol.1995. N 253. P. 309-314.
CrossRef
- Ergun E., Ergun L., Asti R.N., Kurum A. Light andelectron microscopic morphology of Paneth cells in thesheep small intestine . Rev. Med. Vet. 2003. 154, N 5. P. 351-355.
- Ganz T. Defensins: antimicrobial peptides of innate immunity. Nat. Rev. Immunol. 2003. N 3. P. 710-720.
CrossRef
PubMed
- Goessling W., North T.E., Loewer S., Lord A.M., Lee S.Genetic interaction of PGE2 and Wnt signaling regulatesdevelopmental specification of stem cells and regeneration. Cell. 2009. N 136. P. 1136-1147.
CrossRef
PubMed PubMedCentral
- Greenlees K.J., Anadon A. Toxicological evaluation ofcertain veterinary drug residues in food. Lincomycin .Joint FAO/WHO Expert Committee on Food Additives:(15-20 February 2000, Geneva). Genewa: WHO,2000. P. 13-29.
- Gustafsson B.I., Delbro D.S. Effects of indomethacin onnon-adrenergic, non-cholinergic motility of stomachand small intestine . Eur. J. Pharmacol. 1988. 147,N 1. P. 67-72.
CrossRef
- Herias M.V., Midtvedt T., Hanson L.A., Wold A.E. Escherichiacoli K5 capsule expression enhances colonizationof the large intestine in the gnotobiotic rat . Infect. Immun.1997. 65, N 2. P. 531-536.
PubMed PubMedCentral
- Honda A., Sugimoto Y., Namba T., Watabe A., Irie A.Cloning and expression of a cDNA for mouse prostaglandinE receptor EP2 subtype . J. Biol. Chem. 1993. N 268. P. 7759-7762.
PubMed
- Beregova T.V., Eshchenko Y.V., Bovt V.D., EshchenkoV.A. Zinc and the Secretory Material Content in BloodGranulocytes and in the Basal Parts of Intestine Cryptsduring Stress . Int. J. Physiol. and Pathophysiol. 2011.N 3. P. 211-216.
- Hui-Bin Q., Jin-Yan L., Xin L. Effect of enterokineticprucalopride on intestinal motility in fast rats . World J.Gastroenterol. 2003. 9, N 9. P. 2065-2067.
CrossRef
PubMedCentral
- Jahovic N., Sener G., Cevik H., Ersoy Y., Arbak S., YegenB.C. Amelioration of methotrexate-induced enteritis bymelatonin in rats . Cell. Biochem. Funct. 2004. 22,N 3. P. 169-178.
CrossRef
PubMed
- Jingyun Y., Xinjun H., Xiangcai M., Yu-Ming K. Effectsof Portulaca polysaccharide on the dysbacteria in intestinaltract of mice . FASEB J. 2010. N 24 (Suppl.Abstr. Meeting).
- Johansson M.E., Phillipson M., Petersson J., Velcich A.,Holm L., Hansson G.C. The inner of the two Muc2mucin-dependent mucus layers in colon is devoid ofbacteria . PNAS. 2008. 105, N 39. P. 15064-15069.
CrossRef
PubMed PubMedCentral
- Kleiveland C.R., Kassem M., Lea T. Human mesenchymalstem cell proliferation is regulated by PGE2 through differentialactivation of cAMP-dependent protein kinaseisoforms . Exp Cell Res. 2008. N 314. P. 1831-1838.
CrossRef
PubMed
- Lange S., Delbro D.S., Jennische E. Evans blue permeationof intestinal mucosa in the rat . Scand. J. Gastroenterol.1994. 29, N 1. P. 38-46.
CrossRef
PubMed
- Lin P.W., Simon P.O., Gewirtz A.T., Neish A.S. Panethcell cryptdins act in vitro as apical paracrine regulatorsof the innate inflammatory response . J. Biol. Chem. 2004. 279, N 19.P. 19902-19907.
CrossRef
PubMed
- Liu A.Y., Destoumieux D., Wong A.V., Park C.H. Humanbeta-defensin-2 production in keratinocytes is regulatedby interleukin-1, bacteria, and the state of differentiation. J. Invest. Dermatol. 2002. 118, N 2. P. 275-281.
- McGuckin M.A., Lindyn S.K., Sutton P., Florin T.H.Mucin dynamics and enteric pathogens . Nat. Rev. Microbiology.2011. N 9. P. 265-278.
CrossRef
PubMed
- North T.E., Goessling W., Walkley C.R., Lengerke C.,Kopani K.R. Prostaglandin E2 regulates vertebrate haematopoieticstem cell homeostasis . Nature. 2007. N 447. P. 1007-1011.
CrossRef
PubMed PubMedCentral
- Oppenheim J., Biragyn A., Kwak L., Yang D. Roles ofantimicrobial peptides such as defensins in innate andadaptive immunity . Ann. Rheum. Dis. 2003. 62,N 2. P. 17-21.
- Pinto D., Gregorieff A., Begthel H., Clevers H. CanonicalWnt signals are essential for homeostasis of the intestinalepithelium . Genes & Development. 2003. 17, N 14.P.-1709-1713.
- Seong J.S., Seung W.A., Chang K.H., Byung I.R. Expressions?-defensins in human keratinocyte cell lines . J.Dermatol. Sci. 2001. 27, N 3. P. 183-191.
CrossRef
- Thambiayya K., Kaynar A M., Croix C., Pitt B.R. Functionalrole of intracellular labile zinc in pulmonaryendothelium. Pulm. Circ. 2012. 2,N 4. P. 442-451.
CrossRef
PubMed PubMedCentral
- Thor P., Konturek J.W., Konturek S.J., Anderson J.H. Roleof prostaglandins in control of intestinal motility . Amer.J. Physiol. 1985. 248, N 3 (Pt 1). P. G353-G359.
- van Deventer S. J. Review article: targeting TNF-? as akey cytokine in the inflammatory processes of Crohn'sthe mechanisms of action of infliximab . Aliment.Pharmacol. Ther. 1999. 13, N 4. P. 3-8.
CrossRef
PubMed
- Yamada T., Deitch E., Specian R.D., Perry M.A. Mechanismsof acute and chronic intestinal inflammationinduced by indomethacin . Inflammation. 1993. 17,N 6. P. 641-662.
CrossRef
PubMed
- Yamasaki S., Sakata-Sogawa K., Hasegawa A. Zinc is a novel intracellular second messenger . J. Cell Biol. 2007. 177, N 4. P. 637-645.
CrossRef
PubMed PubMedCentral
|