Українська Русский English

ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2013; 59(3): 25-30


Changes in the kinetics of calcium signals in response to high frequency stimulation in the cultured hippocampal neurons

Moskaliuk AO, Voĭtenko SV, Fedulova SA, Veselovs'kyĭ MS

    O.O.Bogomoletz Institute of Physiology National Academyof Science of Ukraine, Kyiv, Ukraine
DOI: https://doi.org/10.15407/fz59.03.025

Abstract

Dynamic changes in the intracellular free Ca2+ concentration ([Ca2+]i) were studied in hippocampal cultured neurons using fluorescent Ca(2+)-indicator dye Indo-1 and somatic whole-cell recordings. During the tetanus stimulation Ca(2+)-transient increased their amplitude up to a steady-state level during repetitive stimulation. We identified two groups of neurons based on Ca-signal dynamics after the end of stimulation: the first group (n = 24) with the monoexponential decay of [Ca2+]i direct after the end of the tetanus; the second group (n = 32) with the monoexponential delayed [Ca2+]i decay after the end of the tetanus, the duration of delay varied from 1 to 27 s and depended on duration and frequency of stimulation. Peak amplitudes of Ca(2+)-transients were statistically different between the first (1820 +/- 195 nM, n = 24) and the second (2618 +/- 165 nM, n = 23) groups. A linear dependence between decay time constant and frequency of stimulation was found for the second group of neurons only. In all cases when the delayed decay was observed the decay time constant changed reliably after emergence of delayed decay; the average rise made up 41 +/- 8%. We suggest dynamic changes and essential rise in the intracellular free Ca2+ concentration arise from the presence of intracellular low-affinity buffer. This statement is to be further tested using pharmacological approach.

Keywords: calcium signals, Indo-1, hippocampal culturedneurons

References

  1. Aponte Y., Bischofberger J., Jonas P. Efficient Ca2+ buffering in fast-spiking basket cells of rat hippocampus . J. Physiol. 2008. 586. P. 2061-2075. CrossRef PubMed PubMedCentral
  2.  
  3. Bartos M., Vida I., Jonas P. Synaptic mechanisms of synchronized gamma oscillations in inhibitory interneuron networks . Nat. Rev. Neurosci. 2007. 8. P. 45-56. CrossRef PubMed
  4.  
  5. Connors B.W., Gutnick M.J. Intrinsic firing patterns of diverse neocortical neurons . Trends Neurosci. 1990. 13. P. 99-104. CrossRef  
  6. Csicsvari J., Jamieson B., Wise K.D., Buzsaki G. Mechanisms of gamma oscillations in the hippocampus of the behaving rat . Neuron. 2003. 37. P. 311-322. CrossRef  
  7. Fang X., McMullan S., Lawson S., Djouhri L. Electrophysiological differences between nociceptive and non-nociceptive dorsal root ganglion neurones in the rat in vivo . J. Physiol. 2005. 565. P. 927-943. CrossRef PubMed PubMedCentral
  8.  
  9. Fedulova S.A., Vasilyev D.V., Isaeva E.V., Romanyuk S.G., Veselovsky N.S. Possibility of multiquantal transmission at single inhibitory synapse in cultured rat hippocampal neurons . Neuroscience. 1999. 92. P. 1217-1230. CrossRef  
  10. Kawaguchi Y. Physiological subgroups of nonpyramidal cells with specific morphological characteristics in layer II/III of rat frontal cortex . J. Neurosci. 1995. 15. P. 2638-2655. CrossRef PubMed
  11.  
  12. Kawaguchi Y., Kubota Y. GABAergic cell subtypes and their synaptic connections in rat frontal cortex . Cereb. Cortex. 1997. 7. P. 476-486. CrossRef PubMed
  13.  
  14. Lee S.H., Rosenmund C., Schwaller B., Neher E. Differences in Ca2+ buffering properties between excitatory and inhibitory hippocampal neurons from the rat . J. Physiol. 2000. 525. P. 405-418. CrossRef PubMed PubMedCentral
  15.  
  16. Martina M., Schultz J.H., Ehmke H., Monyer H., Jonas P. Functional and molecular differences between voltagegated K+ channels of fast-spiking interneurons and pyramidal neurons of rat hippocampus . J. Neurosci. 1998. 18. P. 8111-8125. CrossRef PubMed
  17.  
  18. McBain C.J., Fisahn A. Interneurons unbound . Nat. Rev. Neurosci. 2001. 2. P. 11-23. CrossRef PubMed
  19.  
  20. Nohmi M., Hua S.Y., Kuba K. Intracellular calcium dynamics in response to action potentials in bullfrog sympathetic ganglion cells . J. Physiol. 1992. 458. P. 171-190. CrossRef PubMed PubMedCentral
  21.  
  22. Nowak L.G., Azouz R., Sanchez-Vives M.V., Gray C.M., McCormick D.A. Electrophysiological classes of cat primary visual cortical neurons in vivo as revealed by quantitative analyses . J. Neurophysiol. 2003. 89. P. 1541-1566. CrossRef PubMed
  23.  
  24. Rudy B., McBain C.J. Kv3 channels: voltage-gated K+ channels designed for high-frequency repetitive firing . Trends Neurosci. 2001. 24. P. 517-526. CrossRef  
  25. Schwaller B. Cytosolic Ca2+ buffers . Cold Spring Harb. Perspect. Biol. 2010. 2. a004051. CrossRef PubMed PubMedCentral
  26.  
  27. Sun D.A., Deshpande L.S., Sombati S., Baranova A., Wilson M.S., Hamm R.J., DeLorenzo R.J. Traumatic brain injury causes a long-lasting calcium (Ca2+)-plateau of elevated intracellular Ca levels and altered Ca2+ homeostatic mechanisms in hippocampal neurons surviving brain injury . Eur. J. Neurosci. 2008. 27. P. 1659-1672 CrossRef PubMed PubMedCentral
  28.    

© National Academy of Sciences of Ukraine, Bogomoletz Institute of Physiology, 2014-2019.