The impact of components of conventional and genetically modified soybeans on parameters of the immune and reproductive systems in female rats
Dolaĭchuk OP, Fedoruk RS, Koval'chuk II
Institute of animal biology , Lviv, Ukraine
The article presents results of research of the content of glycoproteins and their specific carbohydrate components, total protein, hemoglobin, erythrocyte, leucocytes molecules of average mass and circulating immune complexes in the blood of female rats under conditions feeding of conventional and transgenic soybeans. Also the reproductive function and mass coefficient of fetus of the studied animals was analyzed. The studies were conducted in three groups of female rats aged 3 months. Animals of I group (control) were kept on a balanced diet during the study period. Animals of groups II and III (research) received a diet according to the scheme of the control group with the addition of 30% from the nutritional value diet native or transgenic soybean, respectively. We found that soy feeding had no significant effect on hematological data in the second and third experimental groups versus control. However, addition to the diet of soybeans, including genetically modified, has a significant impact on the content of glycoproteins and their specific carbohydrate components, female's fertility and less pronounced impact on the data of functional status of their immune system. A generalized analysis of the results of research leads to the conclusion that there is no definite negative or positive impact of GM soy components on their physiological state compared with animals fed native soybeans.
glycoproteins, circulating immune complexes,molecules of average weight, hematological data, rats, geneticallymodified soybeans, reproductive function.
- Vashchenko V.I., Vashchenko T.N. Tseruloplazmin ot metabolita do lekarstvennogo sredstva . Psihofarmakologiya, biologiya, narkologiya. 2006. 6, N 3. P. 1254-1269.
- Vlizlo V.V., Fedoruk R.S., Ratich I.B. Laboratorni meto-di doslidzhen' u biologii, tvarinnitstvi ta veterinarnii meditsini. L.: SPOLOM, 2012. p. 764.
- Grinevich Yu.A. Opredelenie imunnih komnleksov v krovi onkologicheskih bol'nih . Lab. delo. 1981. N 8. P. 493-496.
- Gromashevskaya L. L. Srednie molekuli kak odin iz pokazatelei metabolicheskoi intoksikatsii v organizme . Lab. diagnostika. 1997. N 1. P. 11-16.
- Zorin N.A., Zorina V.N., Zorina R.M. Rol' al'fa2-makroglobulina pri onkologicheskih zabolevaniyah . Vopr. onkologii. 2004. 50, N 5. P. 515-519.
- European convention for the protection of vertebrate animals used for experimental and other scientific purposes. Strasbourg, 1986. 53 p.
- Fournier T. Medjoubi N., Porquet D. Alpha-1-acid glycoprotein . Biochem. Biophys. Acta. 2000. 1482, N 1-2. P. 157-171.
- Huntoon K., Wang Y., Epplito Ch. The acute phase protein haptoglobin regulates host immunity . J. Leukocyte Biol. 2008. 84, N 1. P. 170-181.
- Kuster B,Hunter A.P., Wheeler S.F., Dwek R.A., Harvey D.J. Structural determination of N-linkcd carbohydrates by matrix-assisted laser desorption/ionization-mass spectrometry following enzymatic release within sodium dodecyl sulphate-polyacrylamide electrophoresis gels: application to species-specific glycosylation of alpha-acid glycoprotein . Electrophoresis. 1998. 19, N 11. p. 1950-1959.
- Lowe J.B. Glycosylation, immunity and autoimmunity . Cell. 2001. 104, N 6. P. 809-812.
- Naito Y. Activation-dependent change in sialic acid species in mouse V cells . Trends Glycosci. and Glyc. 2009. 21, N . 120. P. 237-246.
- Sato C. Chain length diversity of sialic acids and its biological significancetrends in glycoscience and glycotechnology . Ibid. 2004. 16, N 91. p. 331-344.
- Thompson G.M., Cantwell J., Cornell C., Turner G.A. Abnormally fucosylated haptoglobins a cancer marker from tumorburden but not gross liver metastasis . Brit. J. Cancer. 1991. 64, N 2. p. 386-390.
- Wei H., Bowen R., Cai Q., Barnes S., Wang Y. Antioxidant and antipromotional effects of soybean isoflavone genistein . Proc. Soc. Exp. Biol. Med. 1995. 208, N 1. P. 124-132.