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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2013; 59(2): 59-64

Insulin receptor expression in subcellular fractions of muscular and adipose tissue as the factor of the tissue insulin resistance development in rats under conditions of the high-energy diet

Kondro MM1, Halenova TI1, Kuznietsova MIu1, Savchuk OM2

  1. Danylo Halytsky Lviv National Medical University Department of Human Physiology, Ukraine
  2. Teaching and Research Center “Institute of Biology” at Taras Shevchenko Kyiv National University, Ukraine


Nowadays the problem of insulin resistance, which has close cause-effect relations with obesity, diabetes mellitus type 2, metabolic syndrome, etc., is of urgent importance in medicine. We have revealed bidirectional changes of the IR content in crude membrane and cytosol of the adipose tissue cells in rats under conditions of the long-term high-energy diet. It is possible that reduction of the IR content in the adipose tissue cells has been predetermined by the disruption of lipid bilayer of adipocytes as a result of peroxidation processes activation. Increase in the IR content in the cytosol of cells of this tissue may indicate the activation of synthesis of this protein; however, it is possible that the IR translocation process disorder occurs due to the damage of plasma membrane, preventing the transfer of newly synthesized molecules of the receptor to the membrane and causing their accumulation in cytosol. The obtained results show that the tissues react to the long-term consumption of high-energy food in different ways. Thus, the content of insulin receptors in the plasma membrane of the muscle tissue cells increases, and, on the contrary, it decreases in adipose tissue cells. Such results may indicate that IR development at the late period of the experiment is likely the result of the adipose tissue cells disfunction. The obtained data may be of high significance in understanding the mechanism of the IR development under conditions of the long-term consumption of the high-energy food.

Keywords: High-Energy Diet, Insulin Resistance, Insulin Receptor


  1. Ketti D. Antitela . Metodi. Kniga 1. M.: Mir, 1991. P. 288.
  3. Kot L.I. Bogdanova O.V., Ostapchenko L.I. Suchasni uyavlennya pro biohimichni mehanizmi patogenezu insulinnezalezhnogo tsukrovogo diabetu . Visn. NAN Ukraini. 2008. N 9. P. 18-26.
  5. Skibchik V.A. IR: klinichne znachennya, metodi viznachennya, pidhodi do likuvannya . Ukr. med. chasopis. 2006. 6, N 56. P.61-67.
  7. Ribal'chenko V.K. Koganov M.M. Struktura i funktsii membran . Praktikum. K.: Vishcha shkola, 1988. P. 312. Osterman L.A. Hromatografiya belkov i nukleinovih kislot. M.: Nauka, 1985. P. 536.
  9. Antti Virkamaki, Kohjiro Ueki, S. Ronald Kahn. Proteinprotein interaction in insulin signaling and the molecular mechanisms of insulin resistance . J. Clin. Invest. 1999. 103(7). P. 931-943. CrossRef PubMed PubMedCentral
  11. Bruning J.C., Winnay J., Bonner-Weir S., Taylor S.I., Accili D., Kahn C.R. Development of a novel polygenic model of NIDDM in mice heterozygous for IR and IRS-1 null alleles . Cell. 1997. 88(4). P. 561-72. CrossRef  
  12. Chad R. Hancock, Dong-Ho Han, May Chen, Shin Terada, Toshihiro Yasuda, David C. Wright, and John O. Holloszy. High-fat diets cause insulin resistance despite an increase in muscle mitochondria . PNAS. 2008. 105, N 22. P. 7815-7820. CrossRef PubMed PubMedCentral
  14. Jellinger P.S. Metabolic consequences of hyperglycemia and insulin resistance . Clin. Cornerstone. 2007. N 8. P. S30-42.  CrossRef  
  15. Huse K., Bohme H.J., Scholz G.H. Purification of antibodies by affinity chromatography . J. Biochem. Biophys. Methods. 2002. 51, N 3. P. 217-31.  CrossRef  
  16. Pessin J.E., Saltiel A.R. Signaling pathways in insulinaction: molecular targets of insulin resistance . J. Clin. Invest. 2000. 106(2). P. 165-169. CrossRef PubMed PubMedCentral
  18. Vasudevan A.R., Garber A.J. Insulin resistance syndrome . Minerva Endocrinol. 2005. 30(3). P. 101-119. PubMed
  20. Pandona P, Aliada A., Chaudhuri A. Metabolic syndrome. A comprehensive perspective based on interactions between obesity, diabetes and inflammation . Circulation. 2005. 110, N 11. P. 1448-1454. CrossRef PubMed
  22. Yang H. Obesity accelerates thymic aging . Blood. 2009. 114(18). P. 100-110. CrossRef PubMed PubMedCentral

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