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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2013; 59(2): 52-58


Mitokorrektine stimulates angiogenesis in vitro

Harmanchuk LV1, Makarenko OM1, Khranovs'ka NM2, Nikolaienko TV2, Nikulina VV3, Nepyĭvoda KhD3, Ostapchenko LI1, Morozov SH4, Kositsyn MS4

  1. NSC «Institute of Biology,» Kyiv National Taras ShevchenkoUniversity, Kyiv, Ukraine
  2. National Cancer Institute, Kyiv, Ukraine
  3. The Institute of Higher Nervous Activity andNeurophysiology RAS, Moscow, Russia
  4. Institute of General Pathology and Pathophysiology RAS,Moscow, Russia
DOI: https://doi.org/10.15407/fz59.02.052

Abstract

The effect of mitokorrectine (complex native oligopeptides isolated from neonatal pig brain) on endothelial cells in culture was investigated. It was revealed that the drug concentration-dependently induces angiogenesis in vitro. Mitogen effect of Mitokorrectine was shown by MTT-test and routine cell count in concentration diapason (0.1-1 mg/ml) which means an increased number of cells by 25 +/- 5% and cell subpopulation of proliferative pool (G2/M+S) 1,8 times in concentration diapason mitokorrectine (0.01-0.05 mg/ml) in comparison with control. In 3-D culture and in stationary phase we detected induction of differentiation of endothelial cells, a decrease the level of NO production and enhancement of glucose metabolism and stimulation of formation of capillary-like tubes.

Keywords: mitokorrectin, angiogenesis, endothelial cells.

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